Protein

Protein accession
A0A8S5NZF7 [UniProt]
Representative
4xoGF
Source
UniProt (cluster: phalp2_1955)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNADEKIWRYLKSAGLNDFGVAGLMGNLFAESGLNPKNLQNTYEKKLGMTDEEYTAAVDSGSYSNFVKDSAGYGLAQWTYWSRKDALLASCKAAGASVGDMDAQLNFLLKELSVGYSGLLSTLKSASSVREASNAVLLQFERPANQGQSVQEKRASYGQAYYDKFAGKIQINTPEQEGGCKLKIVDNLTTVNFRSGNMTPKYIVIHYFGALGTAKGVSEYFKTPGIQASAHYALDEGDTIYRCVRDKDIAWHCGANKYKHPECRNSNSIGIEARPSKINRKRVMASDTDWYFEPKVVDNLVWLTKKLMAQYNIPADHVIRHYDVTGKLCPRPWCCADMNVYYRTSGDAQWEEFKKRISDGKEEDEDMTLDTFKELMKEYRAELQDNDCGTWSKEAREWAISNGLINGTGTEVNGEPNYAWADQLTREQAAALFYRFAKLMGKA
Physico‐chemical
properties
protein length:443 AA
molecular weight:49722,3 Da
isoelectric point:6,37
hydropathy:-0,56
Representative Protein Details
Accession
4xoGF
Protein name
4xoGF
Sequence length
228 AA
Molecular weight
24893,74030 Da
Isoelectric point
8,26822
Sequence
MMNTEEKIWSYLKGQGLTDAGTAGLMGNLYAESGLRANNLQNSYEGKLGMADAEYTELVDKGSYTNFARDSAGYGLCQWTYWSRKEALLAYAKAAGKSIGDLEMQLDFLMKELSTGYMVVLTTLKTATSVRAASDAVMLQFERPADQSETAKARRASYGQKYFDKFAGKGSQTVNFVPRLTRPEAGNKYYITKAAGGYSDAVKGSPTDSACNVLANCVGYAYGRFNEI
Other Proteins in cluster: phalp2_1955
Total (incl. this protein): 24 Avg length: 243,9 Avg pI: 7,12
Protein ID Length (AA) pI
4xoGF 228 8,26822
1Iovj 201 4,81735
1jGOj 247 6,04519
2XK9Y 227 9,15240
2lDLd 236 6,63751
2ok8x 235 8,72601
38MMJ 173 9,49247
3Q0sY 236 5,36949
3TEUl 212 4,99668
3WKVM 215 5,02828
3dOQd 206 4,75028
3pIyw 233 8,69409
40iDS 249 7,70313
41q1w 210 4,80377
4MebO 247 8,42481
5UIOj 276 8,03574
6ci5D 224 8,78177
7DHwi 247 8,79131
8evRz 181 6,38026
8m0Lv 247 8,79196
c9zF 207 5,06279
omSx 231 9,36012
H7BUY1 443 6,36804
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_6190
5KbJP
 74 67,4% 172 5.036E-87
2 phalp2_9140
6h8Qx
 4 67,0% 170 3.404E-83
3 phalp2_18242
4UeaR
 8 61,3% 207 3.404E-83
4 phalp2_19569
3VG9W
 31 60,8% 166 1.377E-77
5 phalp2_31702
3nLVw
 4 56,8% 174 2.630E-74
6 phalp2_26342
1gD1I
 16 55,4% 155 3.401E-64
7 phalp2_39699
a2Er
 1 57,9% 157 5.207E-62
8 phalp2_9130
5YT38
 2 46,7% 186 1.216E-57
9 phalp2_16715
N0Yx
 1 58,7% 143 1.501E-56
10 phalp2_38855
1JedZ
 48 43,1% 183 8.029E-54

Domains

Domains [InterPro]
Phage_lys2
Unannotated
Representative sequence (used for alignment): 4xoGF (228 AA)
Member sequence: A0A8S5NZF7 (443 AA)
1 228 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF18013

Taxonomy

  Name Taxonomy ID Lineage
Phage Myoviridae sp. ct7Q419
[NCBI] 		2825038 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015294 [NCBI]
CDS location
range 2088 -> 3419
strand -
CDS
ATGAACGCCGACGAAAAAATCTGGCGCTATCTGAAATCTGCTGGTCTGAATGATTTCGGCGTCGCGGGTTTGATGGGAAATCTCTTTGCAGAAAGCGGGCTGAATCCCAAGAACCTCCAAAATACATACGAGAAGAAACTTGGCATGACTGATGAAGAATATACTGCCGCCGTCGATAGCGGCAGTTATTCCAACTTTGTGAAAGACAGTGCCGGTTACGGATTAGCTCAGTGGACGTACTGGTCACGCAAGGACGCTCTCCTTGCCTCCTGTAAAGCCGCAGGAGCGTCCGTAGGGGACATGGATGCCCAGCTCAACTTCCTGCTTAAAGAGCTGTCTGTGGGCTATTCTGGGCTGCTGAGCACCCTCAAGAGCGCATCGTCTGTCCGTGAGGCATCCAATGCTGTTCTTCTCCAATTTGAACGTCCTGCCAATCAGGGACAGAGCGTCCAAGAAAAACGAGCCAGCTACGGACAAGCTTATTACGACAAGTTCGCTGGCAAAATCCAAATCAATACACCAGAACAGGAAGGAGGATGCAAATTGAAAATTGTAGACAACCTGACAACGGTTAACTTCCGTTCAGGCAACATGACCCCGAAGTACATCGTTATCCATTATTTCGGTGCACTCGGAACAGCGAAGGGTGTCTCTGAGTATTTCAAGACACCGGGCATTCAGGCATCTGCCCATTATGCGCTTGATGAAGGCGATACAATCTATCGCTGTGTCCGCGACAAGGACATCGCATGGCACTGTGGTGCAAACAAGTATAAGCATCCAGAATGCCGTAACTCTAACTCCATCGGAATTGAAGCACGCCCTTCCAAAATCAATCGCAAGAGGGTTATGGCTTCTGATACCGATTGGTATTTTGAACCAAAAGTTGTGGACAACCTCGTATGGTTGACAAAGAAGTTGATGGCTCAGTACAACATTCCTGCAGACCACGTTATCCGTCATTATGATGTAACCGGAAAACTCTGTCCGAGACCGTGGTGTTGCGCCGACATGAATGTCTATTACAGGACGAGCGGTGATGCGCAGTGGGAAGAGTTCAAAAAGAGAATCAGCGACGGTAAAGAGGAGGATGAAGATATGACTTTGGACACATTCAAGGAACTGATGAAGGAGTACCGTGCGGAGCTGCAGGACAATGACTGCGGCACTTGGAGCAAAGAAGCTCGTGAGTGGGCTATCTCCAACGGTCTCATCAATGGCACTGGCACTGAGGTGAATGGTGAACCCAACTATGCTTGGGCTGACCAGCTTACCCGTGAACAGGCTGCTGCTTTGTTCTATCGTTTTGCAAAACTGATGGGTAAAGCGTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (4xoGF) rather than this protein.
PDB ID
4xoGF
Method AlphaFoldv2
Resolution 93.97
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50