Protein

Protein accession
A0A8S5NKZ2 [UniProt]
Representative
6iJDX
Source
UniProt (cluster: phalp2_20824)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSDLGIKANADVAGCLSKPHPTRWVHIGRRLALVASTVAIALALAVPTTGYAYERITNYVSNGHGPLSPQYLVIHETANPGASAWNHVLLWSRDDTYAVHDVMELDGSKVYDTVPQNRLCWHVGNGNWCTIGIELAHATNAIDFAKQWTEAVKWAGDTLRAHGWDTSRLLSHYEAARIWGGSDHTDPIGYFRQYGKTWNDFKRDVAAYMGSGYIAPIAPTDGNGGTYQPSTSATRTSFPKSTGKSVNIHYALHNRYGAWNSAVTNFNDSNSEGFAGVPYGSHDMLIAWADSGTLRYRVHTKESGWLDWVQAANYNNSVNGMAGIWGQVIDCVQMYYITPNGDYKQVYYRSQDVAHAGYWDEVCDDGTTYGGDDYAGAYGYALDRLQCYVSDGTRR
Physico‐chemical
properties
protein length:395 AA
molecular weight:43657,7 Da
isoelectric point:6,03
hydropathy:-0,42
Representative Protein Details
Accession
6iJDX
Protein name
6iJDX
Sequence length
267 AA
Molecular weight
29556,70360 Da
Isoelectric point
7,17890
Sequence
MTETKAEAKRKLPMRSVFAIVLALVAALAAPCGAEAYQSVDKYVSGGHGYLNASYLVIHETANPGASAYNHTLLWSRDDTYAVHHVMELDGSTVYNTVAENRLCWHVGNGNGYTVGIELAHATNAADFSKQWGEAVKWAGDELRAHGWDTSRLLSHYEAAQRWGGSDHTDPNGYFAQYGKSWWEFKQAVASYLGSGYVAPIAPTNGNGGTYEGGRLQLTGGQLPEIDWRLRECPLCAAQPRRRLELRGDELRRLQRERLRGHALRLP
Other Proteins in cluster: phalp2_20824
Total (incl. this protein): 3 Avg length: 309,0 Avg pI: 7,19
Protein ID Length (AA) pI
6iJDX 267 7,17890
6qHMZ 265 8,35402
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_32212
71H4R
 9 49,3% 166 1.192E-46
2 phalp2_11786
8bZ18
 2 44,2% 165 1.286E-44
3 phalp2_7826
aMK
 29 24,8% 181 2.071E-04
4 phalp2_25092
1jplP
 8 23,6% 207 3.666E-04

Domains

Domains [InterPro]
Disordered region 1
Ami2
Disordered region 2
Representative sequence (used for alignment): 6iJDX (267 AA)
Member sequence: A0A8S5NKZ2 (395 AA)
1 267 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctFbs2
[NCBI] 		2826213 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015193 [NCBI]
CDS location
range 18673 -> 19860
strand -
CDS
GTGAGTGATTTGGGTATCAAGGCAAATGCCGATGTGGCTGGATGTCTATCTAAGCCTCACCCTACTCGGTGGGTACATATCGGTCGACGATTGGCACTCGTCGCTAGTACTGTAGCTATCGCACTGGCACTCGCAGTACCGACAACGGGTTATGCCTACGAGCGCATCACCAATTACGTTAGCAATGGGCACGGGCCGCTGTCACCGCAATACCTCGTGATCCACGAGACGGCTAACCCTGGCGCGAGCGCATGGAACCACGTGCTTTTGTGGTCGCGTGACGACACTTACGCAGTGCACGACGTTATGGAGCTCGATGGCTCCAAGGTTTACGACACGGTACCGCAAAACCGCTTGTGCTGGCACGTCGGCAATGGCAATTGGTGCACGATCGGCATCGAGCTCGCGCATGCCACGAATGCCATAGACTTCGCCAAGCAATGGACTGAGGCCGTGAAGTGGGCAGGCGATACACTCCGTGCGCACGGTTGGGATACCAGCCGCCTACTCAGCCATTACGAGGCCGCACGCATCTGGGGTGGGTCCGACCATACCGACCCGATCGGTTATTTCCGTCAGTACGGCAAGACTTGGAACGATTTCAAGCGCGACGTCGCCGCCTATATGGGTAGCGGCTATATCGCACCGATCGCACCGACTGACGGAAACGGCGGCACGTACCAGCCGTCGACTTCTGCCACGCGCACGAGTTTCCCGAAATCTACGGGCAAGTCTGTCAATATCCACTATGCCCTCCATAACCGTTATGGCGCATGGAATAGTGCTGTCACCAATTTCAACGACTCCAACAGTGAGGGTTTTGCCGGTGTGCCGTACGGCTCCCACGACATGCTCATTGCATGGGCAGACAGCGGTACCTTGCGCTATCGCGTCCACACCAAGGAAAGCGGCTGGCTTGACTGGGTGCAGGCCGCCAATTACAACAATTCTGTAAATGGCATGGCCGGCATCTGGGGCCAGGTGATTGACTGCGTCCAGATGTATTACATCACGCCAAATGGTGACTATAAACAGGTCTACTACCGTTCTCAGGACGTGGCGCACGCTGGCTACTGGGATGAGGTATGCGACGACGGTACGACTTACGGCGGCGATGATTATGCCGGTGCGTACGGTTATGCACTCGACCGTCTCCAGTGTTATGTGTCCGACGGCACCCGCCGTTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0016020 membrane cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6iJDX) rather than this protein.
PDB ID
6iJDX
Method AlphaFoldv2
Resolution 74.37
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50