Protein

Protein accession
A0A8S5NK08 [UniProt]
Representative
7vPuw
Source
UniProt (cluster: phalp2_9318)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MEIRRDILKSWHRCRPSGTYDKTSITIHSTGNPASSAQNERDWLDNPSNRREASWHYVVDEKGVIQAIPDEEEAWHCGNTIGNRFSIGLEICEGGDREKTLRNAVNFAVQKMKEYGFTLTDIVRHYDWTGKNCPRILIDKKYIKDGMDWEWFMKEIESRLRKEKEMEKVYNWTLEVPEWGRPTVQKLLDKGYLQGNEKGELELTYAMLKLLVINDRAGLYD
Physico‐chemical
properties
protein length:221 AA
molecular weight:25948,1 Da
isoelectric point:6,03
hydropathy:-0,81
Representative Protein Details
Accession
7vPuw
Protein name
7vPuw
Sequence length
257 AA
Molecular weight
27321,70350 Da
Isoelectric point
9,58788
Sequence
MRAVIDHIPKSTPYGRRSGQSMVATTITIHNTGNAKSTARNERAWLTNPSNVARASYHIVVDEREAIECIPVNERALHAGTAAGNNTSIGIEICESGNYAQTLTNAVKLVAAMLRERGWGVDRLRRHYDWSRKICPRLMYDGGSWAGWEAFKARVAAELKPAASPRHPTCRIVVNGKEVASGLLIDGRAYAPLRAVGEAAGHSVGWDNASKTASVGGKPVAGLLLDGVTYVALRAAGEAVGGVVAWDGPSKTASITV
Other Proteins in cluster: phalp2_9318
Total (incl. this protein): 15 Avg length: 225,9 Avg pI: 8,21
Protein ID Length (AA) pI
7vPuw 257 9,58788
3ZOUE 223 8,95822
4UA29 257 9,60445
4kozI 221 8,84753
64weS 217 7,16930
6t5tn 221 6,04360
7DEjJ 218 8,97717
7UiAL 214 9,75099
7dFPX 236 7,15611
7yQ0z 228 8,43345
7yQ3Q 219 6,71032
8cxGL 209 8,21754
8teTl 208 8,22650
aLuh 239 9,39171
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_22227
73VY4
 57 75,4% 163 1.316E-83
2 phalp2_39055
7ordh
 68 57,6% 189 4.632E-83
3 phalp2_23523
75EkF
 74 32,8% 250 2.048E-43
4 phalp2_29544
3KzTO
 225 35,5% 236 1.332E-42
5 phalp2_11198
68WMu
 78 33,5% 182 3.398E-42
6 phalp2_13879
7z7dc
 255 43,9% 164 1.617E-41
7 phalp2_33479
7swgL
 97 35,2% 170 4.420E-39
8 phalp2_18793
1bS71
 24 37,8% 161 3.455E-37
9 phalp2_16558
7rWDg
 20 38,1% 173 5.003E-35
10 phalp2_19131
1JgAd
 45 27,6% 275 8.187E-34

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 7vPuw (257 AA)
Member sequence: A0A8S5NK08 (221 AA)
1 257 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctVqj4
[NCBI] 		2826359 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015189 [NCBI]
CDS location
range 18111 -> 18776
strand +
CDS
GTGGAGATTAGGCGAGATATTTTAAAATCCTGGCATAGATGCAGACCGAGCGGGACATATGACAAGACCTCTATTACCATCCATTCCACCGGTAATCCCGCTAGCAGTGCCCAAAATGAACGGGATTGGCTGGATAATCCATCCAACAGAAGGGAAGCCAGTTGGCATTATGTGGTAGACGAAAAAGGGGTAATACAGGCAATTCCAGACGAGGAAGAGGCGTGGCACTGCGGTAACACCATCGGCAACCGGTTTTCTATTGGGCTGGAAATCTGCGAAGGCGGCGACAGGGAAAAAACGCTGAGAAATGCTGTCAATTTTGCTGTGCAAAAAATGAAGGAATACGGGTTTACTCTGACGGATATTGTACGACACTACGACTGGACGGGAAAAAATTGTCCCCGAATCTTAATTGACAAAAAGTATATTAAAGACGGCATGGACTGGGAATGGTTTATGAAAGAGATTGAAAGCCGTTTGAGGAAGGAGAAGGAAATGGAAAAGGTATATAACTGGACGTTGGAGGTCCCTGAATGGGGAAGGCCAACGGTGCAGAAGCTGCTGGACAAAGGATATTTACAAGGCAATGAAAAAGGCGAGTTAGAATTGACCTATGCTATGCTGAAACTGCTTGTCATTAACGATAGAGCAGGGCTATATGACTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7vPuw) rather than this protein.
PDB ID
7vPuw
Method AlphaFoldv2
Resolution 95.63
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50