Protein

Protein accession
A0A8S5NDY2 [UniProt]
Representative
13aRO
Source
UniProt (cluster: phalp2_1330)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGYSALTNAVIMSPNHSGSRYNSISKITIHHMAGNLSIETCGNVFLNPNRQASSNYGIDSDGRIACYVDEENHPWTSANWENDDRAITIEVANSETGGDWPISQEAYASLIRLCADICNRYGIYPYYDGTPSATLTEHCMFVATNCPGPTIHAMQVNHVIENDIRAAMAGGAISTPQSTQPVSGDVEDLARRAIAGEFGNGDARHAALGDMYSAVQARINEIYGGIQSTPSYSIDDIAQRVISGEFGNGVDRINALASAGYDNVEVQQRVNELLQGVPSSTQGDMSAIAEAVYRGDYGNGQDRINALRAAGYDPDEVQRAVDRIYYGL
Physico‐chemical
properties
protein length:328 AA
molecular weight:35389,5 Da
isoelectric point:4,59
hydropathy:-0,35
Representative Protein Details
Accession
13aRO
Protein name
13aRO
Sequence length
321 AA
Molecular weight
34767,23580 Da
Isoelectric point
8,66908
Sequence
VRKSPNHYNGRAYKITKITPHHMAGNLTVEQCGSIFANSARQASSNYGIGTDGRIGLYVDEANASWASSNWDNDNRAVTIEVANSSTGGNWPVSDAAWNSLIALCVDICRRNGIKGLSWTGGSGGSLTCHYMFAPTACPGPYLKGRMAELAKTVNEKLGNGWSPSAPTTKPAGKSLGKVNVSYALKTSKGWLATVTNFENSTSEGYAGIPYGKHYGFWAKTDKGSIRYRVHTVGGGWSGRKRDGQKIETGSPIDGIECYFETPSGYNYQQAWYRSQTTERSGWLDVCCDDGVDIPGYDGWAGMYNEPMDRFQLAIAGGNPF
Other Proteins in cluster: phalp2_1330
Total (incl. this protein): 64 Avg length: 288,3 Avg pI: 5,81
Protein ID Length (AA) pI
13aRO 321 8,66908
19fHm 315 8,64845
1gFq9 307 8,38858
1lPYK 287 7,14247
23I6Q 317 9,70831
24CZI 314 8,84959
2wxH 280 5,38495
3XhVq 287 4,57357
3uJ0T 280 5,29196
3vLEz 280 5,46930
4XSKe 244 5,54609
4YhKv 273 5,11872
4YhTm 278 5,09433
4gJhp 326 5,18777
5MmaX 280 5,73633
5NpZK 280 5,51431
5PPST 280 5,65255
5PVh1 280 5,88945
5PZvi 280 5,88934
5X3uL 280 5,70126
5Xgch 282 5,27684
5YcvG 266 5,27121
5ZNyx 280 5,89047
5ZzMQ 280 5,81977
60L69 280 5,70887
60WPE 280 5,59952
629GS 279 5,72127
62k2H 280 5,51431
63yQr 280 5,46737
65H2J 280 5,51431
65QQF 280 5,48067
65QYk 280 5,55512
68cZs 280 5,56922
6Tiki 283 4,78922
6aXaC 280 5,83739
6aalP 280 5,40660
6bzwy 280 5,46293
6d399 280 5,59758
6gTx8 280 5,81295
6hdQJ 281 5,42008
6jYkf 280 5,58195
6k1O9 282 5,35289
6kKts 280 5,52006
6kKtx 280 5,57297
6lEr9 291 5,90645
6mIk2 280 4,68947
6nTds 280 5,56763
6nuRW 280 5,81425
6okzr 282 5,49601
6qIuQ 280 6,05588
6rpxK 280 5,81704
6to9A 282 5,40518
6u9BF 280 5,54847
6vVAy 280 5,66158
6wz3Y 280 5,81295
7biif 332 4,78547
7dQi2 324 5,08677
7eYIb 322 5,06591
7hXkV 324 5,08677
7wdAD 278 8,61254
86b3s 333 6,59897
8on0d 280 5,46594
8tWtv 331 4,73545
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_5505
3kRYJ
 67 44,6% 327 3.465E-88
2 phalp2_28981
6d4eV
 350 34,9% 343 3.833E-86
3 phalp2_29674
8k36v
 96 53,1% 209 6.219E-76
4 phalp2_19574
3WBUx
 1 41,4% 316 1.949E-74
5 phalp2_12977
3h4lU
 700 48,0% 254 3.104E-67
6 phalp2_8364
oq39
 14 37,7% 342 4.997E-63
7 phalp2_20459
3AV6U
 1 38,0% 297 9.134E-55
8 phalp2_1650
8m87G
 265 41,8% 246 7.997E-49
9 phalp2_30111
3bZzl
 4 28,7% 226 9.795E-35
10 phalp2_36721
6gIHr
 182 24,7% 351 8.284E-32

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 13aRO (321 AA)
Member sequence: A0A8S5NDY2 (328 AA)
1 321 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctxzZ3
[NCBI] 		2826523 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015148 [NCBI]
CDS location
range 640 -> 1626
strand -
CDS
ATGGGATATTCAGCTTTAACCAATGCAGTAATCATGAGTCCTAATCATTCAGGCTCACGCTACAATTCAATCTCAAAGATTACCATCCATCATATGGCTGGCAACCTTTCAATCGAGACGTGTGGAAACGTCTTTTTAAATCCAAACAGACAAGCATCATCTAACTACGGAATTGATTCTGATGGACGTATTGCATGTTATGTCGATGAAGAGAATCATCCGTGGACATCCGCCAACTGGGAAAACGACGACAGAGCAATCACTATTGAAGTTGCGAATAGTGAGACTGGTGGCGATTGGCCAATCAGCCAAGAAGCATATGCATCGCTAATTCGTCTATGTGCAGATATCTGCAATCGCTACGGAATTTATCCATATTATGATGGAACTCCGTCTGCAACATTAACAGAGCATTGCATGTTTGTTGCAACAAATTGTCCTGGTCCTACAATCCATGCTATGCAGGTAAATCATGTTATCGAAAATGATATTCGCGCAGCTATGGCAGGTGGTGCAATTAGTACTCCGCAATCAACGCAACCGGTTAGTGGTGATGTTGAAGATTTAGCACGTAGAGCAATTGCGGGTGAGTTTGGAAATGGTGATGCAAGACATGCTGCATTAGGCGATATGTATAGTGCAGTCCAAGCACGCATTAATGAAATCTATGGTGGTATTCAATCTACGCCATCGTACTCTATTGATGATATTGCACAGAGAGTGATTTCCGGCGAGTTTGGTAACGGTGTAGACCGTATCAATGCATTAGCTTCAGCAGGTTATGACAATGTGGAAGTACAACAACGTGTAAACGAGCTTCTTCAAGGTGTGCCATCTTCAACACAGGGTGACATGAGCGCTATTGCTGAAGCGGTCTATCGCGGTGACTACGGCAATGGCCAAGATAGAATCAATGCTCTACGTGCAGCAGGATATGATCCGGATGAAGTACAGCGTGCAGTAGACCGAATTTACTACGGACTATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (13aRO) rather than this protein.
PDB ID
13aRO
Method AlphaFoldv2
Resolution 89.92
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50