Protein

Protein accession
A0A8S5NCX6 [UniProt]
Representative
3k9WX
Source
UniProt (cluster: phalp2_24292)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKIDRSYISDQNTYAENDPRCIVVHNTDNFRAGADAQTHAEAQHNGELSNMSAHYYVDDGDTAYQAAPHSRGCWHVGVNYGGNNLFGRYGNRNSIGVEMCVQKGYNYEKAFQNTVAVVKEIMCETDIPASRVYRHYDICSKHCPSQIIERGDWERFKSLISGTASAEQPASGKYEPGIYKVNTDLNIREKPNADSSIVGRITDQGSYTVTEIQNTSWGRLLSGAGWINCHTKYCTYGGPASQSDQKPTAKVIAVDGVWGPELTRRLQEIFGTGVDGKISNQPTSNKKYCAGIAAAEWSDKLSGGSDLIKAMQRWAGVTADGYLGPQTIRALQKKLGTPVDGVISYPSAMVRALQEWCNRQ
Physico‐chemical
properties
protein length:360 AA
molecular weight:39643,7 Da
isoelectric point:7,08
hydropathy:-0,59
Representative Protein Details
Accession
3k9WX
Protein name
3k9WX
Sequence length
166 AA
Molecular weight
18580,34640 Da
Isoelectric point
6,43181
Sequence
MKIDRSFISNQNTYEENDPRCIVVHNTDNFRAGADARTHAEAQHNGELSNMSAHYYVDDGETAYQAAPHSRGCWHVGVNYGGNNLFGRYGNRSSIGVEMCVQAGYDYEKAFQNTVALVKEIMRETGIPAARVYRHYDICSKHCPSQIIERGDWARFKSLINGAAAA
Other Proteins in cluster: phalp2_24292
Total (incl. this protein): 42 Avg length: 211,1 Avg pI: 7,82
Protein ID Length (AA) pI
3k9WX 166 6,43181
13sgw 205 8,88492
13us7 205 8,87177
13x5L 205 6,74954
16UeQ 244 9,14750
2ZO2b 243 8,37143
2bbil 211 8,73967
2c608 205 7,67062
2fXdG 217 8,46046
3KN7d 242 8,58811
3PBi1 214 5,78203
3nJEZ 176 9,47493
3nPHP 206 7,18851
3nSRp 218 8,32952
3o11O 215 7,03646
3o8C5 218 8,56290
3q9XX 248 8,73742
3uiPG 207 8,22405
3wVRN 155 9,34787
45e1X 207 8,25236
45zeT 212 7,83563
47OM9 214 5,88837
4A3xe 219 6,97951
4NR8B 203 8,47677
4UYrT 229 8,22515
4mtZn 230 9,47906
4mu3H 205 6,76858
4tIVh 238 9,46114
4tJ0U 198 9,60993
5MeiK 248 5,91617
5SXc3 184 6,22338
63H82 213 7,03470
6M1GA 205 8,73703
6UJiP 213 9,03494
6fKaf 235 5,98505
6jo15 131 5,79095
6m59D 120 5,80453
6td95 106 6,34490
79LpP 212 9,09844
7qvPn 247 7,65123
8rOu7 239 8,28646
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_38485
1mrCF
 6 63,6% 165 2.363E-62
2 phalp2_24299
3nIle
 36 34,3% 160 1.327E-46
3 phalp2_29544
3KzTO
 225 38,5% 153 5.827E-45
4 phalp2_259
6ql8e
 14 39,1% 161 1.236E-42
5 phalp2_7826
aMK
 29 38,6% 158 1.693E-42
6 phalp2_29527
23SbO
 38 35,6% 160 2.321E-42
7 phalp2_30401
4NIg7
 17 42,1% 121 4.914E-40
8 phalp2_33479
7swgL
 97 37,1% 148 6.733E-40
9 phalp2_29669
8sfID
 1005 30,0% 163 6.733E-40
10 phalp2_1605
81mMl
 270 28,3% 180 2.373E-39

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 3k9WX (166 AA)
Member sequence: A0A8S5NCX6 (360 AA)
1 166 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Myoviridae sp. ct5Tq8
[NCBI] 		2826612 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015138 [NCBI]
CDS location
range 200 -> 1282
strand -
CDS
ATGAAAATTGACAGATCCTATATCAGCGATCAGAACACCTACGCCGAAAACGATCCACGGTGTATCGTGGTACACAATACGGATAATTTCAGAGCAGGTGCAGATGCCCAAACACATGCAGAAGCGCAGCATAATGGTGAGCTGTCCAATATGTCTGCCCACTATTACGTTGATGACGGAGATACAGCATACCAGGCGGCGCCGCACAGCCGCGGATGCTGGCACGTGGGCGTAAACTATGGTGGAAATAATCTGTTTGGCCGATACGGCAACCGTAACAGCATCGGGGTAGAGATGTGCGTGCAGAAGGGGTATAATTATGAAAAAGCGTTTCAGAACACGGTGGCCGTGGTCAAGGAGATCATGTGTGAGACCGACATCCCGGCGAGCCGCGTATACCGTCATTATGATATCTGTAGCAAGCACTGCCCGAGCCAGATCATCGAGAGAGGGGATTGGGAGCGATTTAAGAGCCTGATCAGCGGCACGGCATCGGCCGAACAGCCAGCAAGCGGAAAATATGAGCCTGGTATCTACAAGGTTAATACCGACCTTAATATTAGAGAGAAGCCGAACGCAGACAGCAGTATCGTTGGCAGGATCACGGATCAGGGAAGCTATACGGTAACAGAAATCCAGAATACAAGCTGGGGACGGCTGCTCTCTGGTGCGGGCTGGATCAACTGCCACACCAAGTATTGTACTTACGGTGGCCCTGCATCTCAGTCCGATCAGAAGCCTACCGCAAAAGTGATTGCGGTTGATGGCGTGTGGGGTCCGGAACTGACCCGGCGTCTGCAGGAGATTTTCGGAACTGGCGTAGACGGGAAAATCAGTAATCAGCCCACGAGCAACAAAAAATACTGCGCTGGCATCGCGGCGGCCGAATGGTCTGATAAACTGTCCGGCGGCTCCGATCTGATCAAGGCCATGCAGAGATGGGCAGGAGTGACCGCAGACGGCTACCTCGGACCGCAGACCATCCGCGCGCTCCAGAAAAAACTCGGCACACCGGTAGACGGCGTGATCAGCTACCCGTCAGCGATGGTGCGCGCCCTGCAGGAGTGGTGCAATCGGCAGTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (3k9WX) rather than this protein.
PDB ID
3k9WX
Method AlphaFoldv2
Resolution 96.43
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50