Protein

Protein accession
A0A8S5N0C9 [UniProt]
Representative
DDEb
Source
UniProt (cluster: phalp2_35500)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTEIYSNYFENGVYFTSPKNNILGVVIHNDAGSLSARQYDGFLVGRVNNGTLANGFAAYYVDRNDVYVFQPTNRQEWHTANAYGNANFIGFEVCQSMSASDSEFIANEDATLLLAGQVLQSYGLPINEDTVKLHHEFSSTSCPHRSMELHGNGGAYNGAGTEACRQYFINRIKQLLAGDVSEPTVVEKSILDEDVELVKRDEPYYEATVSIDYILESQPTEDSEDKEFVPAGTRVRVYEKKDGWSRVNYKDSDQWIEDKYLTEVTVF
Physico‐chemical
properties
protein length:267 AA
molecular weight:30092,7 Da
isoelectric point:4,57
hydropathy:-0,49
Representative Protein Details
Accession
DDEb
Protein name
DDEb
Sequence length
271 AA
Molecular weight
30678,32730 Da
Isoelectric point
4,58898
Sequence
MADIYSSYFKQGIYFAPPKNSIRGIVLHNDAGGNSARQYEGFLRDRVNNGTLANGFAAYYVDRNDTFVFQPVNYQEWHTATYEGNANYIGIEACQSMSASDEDFIANEDAALMIAGELLESYGLPVNEDTVRLHHEFSPTACPHRSMELHANGGAYYGAGTQNCKAYFIDRIKKLRSGEIEIGDTTDVAEVVEKSILDEDVELPKREQAYYEATVSIDYYLESQPSLDSEDKEFVAAGTRVRVYEKKDGWSRVNYKDSDQWIEDKYLTECE
Other Proteins in cluster: phalp2_35500
Total (incl. this protein): 68 Avg length: 267,3 Avg pI: 4,57
Protein ID Length (AA) pI
DDEb 271 4,58898
11x2X 271 4,56130
2bjjP 271 4,56851
3c73p 271 4,65053
3c8ie 264 4,65303
5KtXB 267 4,57727
5Nsf9 267 4,48036
5XEXt 267 4,50008
638RX 271 4,57119
63oS4 271 4,57732
65NzM 263 4,54475
6YsxR 271 4,66815
6ZU8s 271 4,65053
6ZjGW 271 4,60358
6ZpjL 270 4,49315
6amEv 267 4,47791
6g1Ba 271 4,52782
6id01 271 4,59455
6nDcD 267 4,41636
6nLqw 267 4,54208
6pkLC 270 4,60336
6qTL7 264 4,70356
6ud3g 267 4,54186
6ujQx 263 4,61285
70u63 271 4,57732
71btU 270 4,68572
7M5Ow 267 4,54208
7MMlS 267 4,48746
7MXpx 271 4,65053
7Ml9Z 271 4,54896
7MnE0 271 4,57596
7OQ2j 271 4,60358
7OQ7Z 271 4,55112
7OsIt 206 4,61023
7UOQb 268 4,55612
7XZgT 271 4,64161
8bqU6 268 4,48343
8hc8M 271 4,62228
8lqmM 271 4,56851
BVg0 271 4,59477
CEGC 267 4,49923
CUob 271 4,55112
DTHj 275 4,63502
F71t 267 4,46905
HExU 271 4,52469
HoSF 267 4,47791
Honr 267 4,53293
JYo3 271 4,58414
JZ6Q 271 4,53356
JZF5 271 4,52469
K5FZ 267 4,53293
K8EN 267 4,50912
K8ER 267 4,55118
NaCC 271 4,58414
Nrld 271 4,65053
O3XV 267 4,46012
OLhf 270 4,68572
Obcw 271 4,54896
oAxI 271 4,55112
oCky 267 4,49781
oH4I 264 4,70356
oQp5 267 4,54186
oVX8 267 4,55828
rlLy 262 4,65707
tpV4 268 4,49525
wJpD 271 4,65940
x1iP 221 4,47683
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_36896
7vCZc
 30 43,0% 181 2.760E-44
2 phalp2_36839
6Z1Os
 11 38,7% 173 1.270E-33
3 phalp2_15359
1El58
 3 34,8% 215 1.348E-29

Domains

Domains [InterPro]
Ami2
SH3_3
Representative sequence (used for alignment): DDEb (271 AA)
Member sequence: A0A8S5N0C9 (267 AA)
1 271 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08239

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ct8eQ1
[NCBI] 		2826171 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015025 [NCBI]
CDS location
range 35933 -> 36736
strand +
CDS
ATGACAGAAATTTATAGTAATTACTTTGAAAACGGAGTGTACTTCACATCCCCAAAAAACAACATTTTAGGAGTTGTAATTCACAATGACGCAGGGAGTTTGTCAGCTAGACAATATGACGGATTTTTAGTAGGACGAGTGAACAACGGAACATTAGCTAATGGTTTTGCTGCATACTATGTAGACCGTAACGACGTTTACGTTTTCCAACCAACTAATCGCCAAGAATGGCACACAGCCAACGCTTACGGTAATGCTAATTTTATTGGTTTTGAAGTGTGTCAATCTATGTCAGCAAGTGACAGCGAGTTTATAGCAAACGAAGACGCAACGCTACTATTAGCAGGTCAAGTCTTACAAAGTTACGGATTACCAATTAATGAAGATACGGTCAAACTACACCATGAATTTAGCTCAACATCATGTCCACATAGAAGTATGGAACTTCACGGAAATGGTGGAGCATACAACGGAGCAGGTACAGAAGCGTGTCGACAATACTTCATTAATAGAATTAAGCAACTATTAGCTGGAGACGTAAGCGAACCAACAGTTGTTGAGAAAAGTATTCTTGATGAAGATGTTGAACTTGTTAAACGTGATGAACCTTATTACGAGGCAACAGTAAGCATTGATTACATTCTTGAAAGCCAACCAACAGAAGATAGTGAGGATAAAGAATTTGTCCCAGCTGGAACTAGAGTGAGAGTCTATGAGAAAAAAGACGGTTGGAGTAGAGTAAATTATAAAGATAGCGATCAATGGATAGAAGATAAGTATTTAACTGAAGTTACAGTATTTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (DDEb) rather than this protein.
PDB ID
DDEb
Method AlphaFoldv2
Resolution 91.83
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50