Protein

Protein accession
A0A8S5MVV8 [UniProt]
Representative
7Ys8w
Source
UniProt (cluster: phalp2_31217)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MRTSVLGRLGEVIVTDWHNLIADYNLWFDNYDEGRSRALDRVIVHHNAGKAMSHGGVLAAFNNNGTSAHYNVDIDGSTAQFVHDEDTAWHCPGVNSCSIGIEHANSTGAEGGWDIGEATLDAGAHLTAALCRAYGLGRPQWRVNVFPHSDFYSTACPASLRDKYAGEYMEKAQAYYDNLDAEIIQSEGWVSQDGGWWYRTSDGGWETGWFPVNDKWFYANEKGWLQAGWQHIDGHWYFLHDMHDSRYGEMETGWQKIGENWFLLNDKGQMQTGWQLVKGKWYFLEENGAMRTGWLSYNGNDYFLTETGAMAVGLCQTRLDGACSIFGEDGKLLVGKLVVEQDADGIVKLVESK
Physico‐chemical
properties
protein length:353 AA
molecular weight:39623,3 Da
isoelectric point:4,89
hydropathy:-0,47
Representative Protein Details
Accession
7Ys8w
Protein name
7Ys8w
Sequence length
164 AA
Molecular weight
18900,87120 Da
Isoelectric point
4,83174
Sequence
MSQDSGWWYRNADGGYETGWFPVGDKWYYANEKGWLQFGWQLVDGHWYFLHDVHDGRYGEMETGWVKVGEHWFLLNDKGQMQTGWQLNKGKWYFLEENGAMRTGWLSYGGEDYFLTETGAMAVGLVQTRLDGACSIFGEDGKMLKGRIVVEQDADGIVKLVESK
Other Proteins in cluster: phalp2_31217
Total (incl. this protein): 6 Avg length: 271,5 Avg pI: 4,85
Protein ID Length (AA) pI
7Ys8w 164 4,83174
8fL55 209 4,73659
8nd9h 209 4,94450
A0A8S5N1J7 341 4,78950
A0A8S5UN34 353 4,88926
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_32236
7mjU4
 1 42,0% 119 3.892E-29
2 phalp2_9808
8hjWr
 2 31,4% 175 9.974E-23
3 phalp2_28277
OqMD
 47 33,8% 124 7.169E-20

Domains

Domains [InterPro]
Choline_bind_3
Representative sequence (used for alignment): 7Ys8w (164 AA)
Member sequence: A0A8S5MVV8 (353 AA)
1 164 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF19127

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctsus30
[NCBI] 		2826488 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014997 [NCBI]
CDS location
range 1857 -> 2918
strand +
CDS
ATGAGGACTTCGGTCCTTGGGAGATTAGGAGAAGTTATAGTGACTGACTGGCATAATCTGATCGCTGATTACAACCTGTGGTTCGATAACTACGATGAGGGGCGTAGTCGAGCACTTGACCGTGTTATCGTCCACCATAACGCTGGCAAGGCTATGTCGCATGGTGGTGTCCTTGCAGCGTTTAACAATAATGGCACGTCTGCGCACTACAACGTGGACATTGACGGCAGCACCGCACAGTTCGTCCACGACGAGGACACGGCCTGGCACTGCCCAGGCGTTAACTCGTGTTCGATTGGCATTGAGCACGCTAACTCCACTGGCGCTGAGGGTGGCTGGGACATTGGTGAAGCGACGCTTGATGCCGGTGCGCACCTGACTGCGGCTCTGTGTCGTGCGTATGGTCTTGGCCGTCCGCAGTGGCGTGTCAATGTGTTCCCCCACTCGGACTTCTATTCGACTGCCTGCCCTGCGTCTCTGCGGGATAAGTACGCTGGTGAGTACATGGAGAAGGCTCAGGCGTACTATGACAACCTTGATGCTGAGATCATTCAGTCTGAAGGCTGGGTGTCGCAGGATGGTGGCTGGTGGTACCGCACCTCGGATGGTGGCTGGGAGACCGGCTGGTTCCCTGTGAACGATAAGTGGTTCTATGCCAACGAGAAGGGTTGGTTGCAGGCTGGTTGGCAGCATATCGACGGCCACTGGTACTTCCTGCACGATATGCACGATTCGCGCTACGGCGAGATGGAGACTGGCTGGCAGAAGATCGGTGAAAACTGGTTCCTCCTGAACGACAAGGGACAGATGCAGACCGGCTGGCAGCTCGTCAAGGGCAAGTGGTACTTCCTTGAGGAAAACGGCGCTATGCGTACCGGCTGGCTGTCGTACAACGGCAACGACTACTTCCTCACTGAGACTGGCGCTATGGCTGTCGGCCTGTGCCAGACGCGACTTGACGGTGCTTGCTCCATTTTCGGTGAAGACGGCAAGCTGCTTGTCGGTAAGCTGGTTGTCGAACAGGACGCTGACGGCATCGTGAAGCTGGTAGAATCTAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7Ys8w) rather than this protein.
PDB ID
7Ys8w
Method AlphaFoldv2
Resolution 93.41
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50