Protein

Protein accession
A0A8S5MK30 [UniProt]
Representative
64Cdz
Source
UniProt (cluster: phalp2_33293)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSYTNSKLVSYTKISPNRNINRNHKIDTVSIHCVVGQCSVETLGSIFASRGKEASSNYGIGYDGRIGMYVEEKDRSWCTSSASNDNRAITIEVASDTYHPYKVNDKAYKSLIKLLVDICKRNGIKRLVWSTNKSARMNHLNGCNMTVHRNYANKSCPGDYLYNLHGQIAKEVNAQLGLGSSKPVTSKKTLYRVRKSWKDAKSQKGAFYDLSNAKKCANKNSGYSVFDESGKSVYTPKSSGKKSVDTIAREVIQGKWGNGADRKNRLTKAGYNYNTVQKRVNEILS
Physico‐chemical
properties
protein length:285 AA
molecular weight:31814,6 Da
isoelectric point:9,80
hydropathy:-0,73
Representative Protein Details
Accession
64Cdz
Protein name
64Cdz
Sequence length
389 AA
Molecular weight
41597,68560 Da
Isoelectric point
9,58472
Sequence
MAYTNSPLVSYTKISPNRTKNRNHAIDTITIHCVVGQCSVQTLGNIFAPTSRGASSNYGVGYDGKIGMYVEEKDRSWCSSSSVNDNRAVTIEVASDTSEPYAVNQKAYASLLNLVTDICKRNGIKKLIWSKNKNERVNHLNGCNMTVHRDYANKSCPGTYLYNKHGEIAAEVNKRLGANATTPQAPSANLKFKAGDIVQFAGGKHYKSVDASSGSTVKASKAKITQVASGKHPYHCRAVNEAGAFVSGVYGWVDENTLTAISNVSKPATGSTGLKIGDQVMFTGCLHYTSSYKTATAHACKAGLAKITKISAKNPHPYHLQAVAGKGSTVYGWVGAKDIQATANTYTVKSGDTLSGIAKKYGTTVAKLVELNGIKNANLIYKGQLIKLP
Other Proteins in cluster: phalp2_33293
Total (incl. this protein): 41 Avg length: 342,0 Avg pI: 9,24
Protein ID Length (AA) pI
64Cdz 389 9,58472
139CA 321 9,36037
13ErO 339 9,30010
13Gxe 335 8,93920
1kgCp 336 9,29004
21Fts 275 9,35638
23idq 274 9,46636
24D6k 275 9,45069
2XvmQ 339 9,34342
2bN72 341 8,85746
3pcHt 336 9,35696
4k4Ow 394 9,45237
5LOqu 354 9,10366
5Lpe9 385 9,27637
5QFc7 332 9,38500
5RhZb 270 8,97685
5T531 336 9,23904
5ZdaX 336 9,33704
625KS 270 9,16549
62hGx 336 9,17419
64mZS 336 9,28991
65wPX 303 9,32969
686SV 336 9,29004
68zTI 336 9,27167
6aJ9i 336 9,29004
6bbDa 303 9,33987
6eWcR 389 9,39499
6g56R 335 9,13138
6kLfs 354 9,24472
6mF0Q 336 9,29017
6w8B4 336 9,29004
75GF 329 8,96931
7VUJ9 336 9,29958
7W0Hr 336 9,28991
7W4OQ 480 8,68288
7yIfD 351 8,98207
86kAD 481 8,90935
86qKO 336 9,35696
8b7y1 334 9,41040
8cxrR 480 8,52860
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_30077
2WEC1
 850 62,5% 278 5.181E-122
2 phalp2_12971
3esnk
 626 57,5% 259 1.415E-104
3 phalp2_32522
1n71i
 16 52,5% 274 7.452E-102
4 phalp2_237
66qOT
 25 45,0% 362 5.981E-100
5 phalp2_4373
2br9t
 1 30,5% 530 4.175E-83
6 phalp2_5937
6aZNZ
 614 46,8% 269 1.065E-82
7 phalp2_15173
ovmV
 70 39,4% 294 6.456E-59
8 phalp2_21398
41bpM
 1 37,6% 303 1.362E-46
9 phalp2_13006
40hKf
 5 31,6% 262 1.058E-37
10 phalp2_25522
3iCuM
 5 30,3% 293 1.527E-34

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
LysM
Representative sequence (used for alignment): 64Cdz (389 AA)
Member sequence: A0A8S5MK30 (285 AA)
1 389 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01476, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctMM521
[NCBI] 		2826259 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014922 [NCBI]
CDS location
range 44292 -> 45149
strand +
CDS
ATGTCATACACAAACAGCAAATTAGTTAGCTACACAAAAATTTCACCAAACAGAAACATCAATCGCAATCACAAGATTGATACAGTTTCAATTCACTGCGTTGTCGGACAGTGTTCTGTTGAAACTCTCGGTTCAATCTTTGCGTCAAGGGGCAAGGAGGCAAGCTCAAACTATGGTATCGGATATGACGGCAGAATCGGAATGTATGTTGAGGAAAAGGATCGCAGTTGGTGTACTTCCTCTGCGTCAAATGACAACAGAGCAATTACTATCGAAGTTGCATCCGATACATACCACCCGTATAAGGTAAATGACAAGGCATACAAATCCTTGATTAAGCTGCTTGTTGATATCTGCAAAAGAAACGGCATCAAAAGGCTTGTGTGGTCAACAAATAAGTCAGCGAGAATGAATCATCTTAACGGTTGCAATATGACGGTTCATAGGAATTATGCAAACAAGTCCTGTCCGGGCGATTATCTCTACAATCTTCACGGACAGATTGCAAAGGAGGTGAACGCTCAGCTTGGCTTAGGCAGTTCAAAACCTGTGACATCAAAGAAAACTCTCTATCGTGTACGCAAAAGTTGGAAGGACGCAAAGTCGCAGAAGGGTGCTTTCTATGACCTTTCAAATGCCAAGAAATGTGCCAACAAAAACAGCGGTTACTCTGTTTTTGATGAAAGCGGAAAGAGTGTGTACACGCCAAAGTCATCGGGCAAAAAGTCAGTTGATACCATTGCAAGAGAAGTAATTCAGGGTAAATGGGGCAACGGCGCCGACCGCAAAAACCGCCTCACCAAAGCCGGCTATAACTACAACACCGTTCAGAAAAGAGTAAACGAGATTTTATCTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (64Cdz) rather than this protein.
PDB ID
64Cdz
Method AlphaFoldv2
Resolution 87.97
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50