Protein

Protein accession
A0A8S5MF08 [UniProt]
Representative
8hjWr
Source
UniProt (cluster: phalp2_9808)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 97% (predicted by ML model)
Protein sequence
MGFTVSEFIQCHGSNMDWGRGGKSVSYIAVHYTGTNASGHNNLVYFSGPNRNASAHYFVDTDGTISQSVSERDTAWAVGNYDRNQRSISIECVSDGSAPFTAAQIASLRAIVTDLMYRYGITADRVIRHYDVTGKHCPAQYVDESAWAALKNQITSTYVGEPKWVHSPNGWWYQRSDGSYPANEWMWVTDAWYWFNGQGYMETGWHWINGKCYYLSESDDTIGKMVSGMQSINGAKYFFAPTGELQFGWIKYDGNWYVTDDKWGIIQTSQWHFKDNTWYWLDGDGHMVTGHQTIDGKQYLFDDSGAMQTGLREVEGDTYLFSESGSMVTDTLYINPDTKKLSAFDHDGRMIKNHVLSVNIDENGTVTGIKSID
Physico‐chemical
properties
protein length:373 AA
molecular weight:42173,0 Da
isoelectric point:5,14
hydropathy:-0,52
Representative Protein Details
Accession
8hjWr
Protein name
8hjWr
Sequence length
258 AA
Molecular weight
29868,67090 Da
Isoelectric point
4,99253
Sequence
MYRYGITADRVIRHYDVTGKHCPAQYVDESAWAALQNQITSTYVGEPKWVHSPNGWWYQRSDGSYPANEWMWVTDAWYWFNGQGYMETGWHWINGKCYYLSESDDTIGKMVSGMQSINGAKYFFAPTGELQFGWVKYDGHWYVTDDKWGIIQTSQWYFKDNTWYWLDGDGHMVTGHQTIDGKRYLFDDSGAMQTGLREVEGDTYLFSESGSMVTDTLYINPDTKKLSAFDHDGRMIKNHVLSVNIDENGTVTGIKSID
Other Proteins in cluster: phalp2_9808
Total (incl. this protein): 2 Avg length: 315,5 Avg pI: 5,07
Protein ID Length (AA) pI
8hjWr 258 4,99253
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_31217
7Ys8w
 6 29,4% 207 3.723E-20
2 phalp2_10040
7lBLU
 4 27,6% 264 6.525E-18

Domains

Domains [InterPro]
Unannotated
Representative sequence (used for alignment): 8hjWr (258 AA)
Member sequence: A0A8S5MF08 (373 AA)
1 258 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctWuM9
[NCBI] 		2826364 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014888 [NCBI]
CDS location
range 646 -> 1767
strand +
CDS
ATGGGTTTTACAGTTTCAGAATTTATCCAATGCCATGGCTCTAACATGGATTGGGGTCGAGGTGGAAAAAGCGTAAGCTATATTGCCGTTCACTACACCGGCACTAATGCTTCTGGTCATAACAATCTTGTTTATTTTTCTGGTCCAAACCGCAATGCTTCAGCGCATTATTTTGTTGATACAGACGGTACAATTTCTCAGAGTGTGTCTGAGCGAGACACTGCTTGGGCTGTTGGTAACTACGATCGCAATCAGAGGTCAATCTCTATTGAGTGCGTTTCCGATGGTTCTGCTCCTTTTACAGCTGCTCAAATCGCATCCCTTAGAGCAATCGTAACTGATCTGATGTATCGATACGGAATCACTGCTGATCGAGTTATTCGTCACTACGATGTAACTGGCAAGCATTGCCCAGCACAGTATGTTGATGAGTCAGCTTGGGCTGCGCTTAAGAACCAGATCACTTCAACATATGTTGGAGAGCCAAAGTGGGTTCACAGCCCAAACGGTTGGTGGTATCAGCGTAGCGATGGTTCATATCCAGCGAACGAGTGGATGTGGGTCACTGATGCTTGGTATTGGTTCAACGGTCAAGGATACATGGAGACTGGTTGGCATTGGATTAATGGAAAGTGCTACTATCTTTCTGAGTCTGATGACACAATCGGTAAGATGGTAAGCGGAATGCAATCCATTAATGGCGCTAAGTATTTCTTTGCTCCAACTGGAGAGTTGCAGTTTGGCTGGATCAAGTACGACGGCAATTGGTATGTTACCGATGACAAATGGGGCATCATTCAAACTAGCCAGTGGCATTTCAAGGATAATACTTGGTATTGGCTTGACGGTGATGGTCATATGGTCACTGGTCACCAGACAATTGATGGAAAGCAATACCTATTTGACGACTCTGGCGCAATGCAGACAGGACTTCGTGAAGTAGAAGGCGATACATATTTGTTTAGCGAGTCTGGTTCGATGGTAACTGACACACTTTACATCAATCCTGATACAAAGAAGCTCTCGGCATTTGATCATGATGGTCGAATGATTAAGAATCATGTTCTCTCGGTCAACATTGACGAGAACGGAACTGTCACAGGCATCAAGAGTATCGATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8hjWr) rather than this protein.
PDB ID
8hjWr
Method AlphaFoldv2
Resolution 91.65
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50