Protein

Protein accession
A0A8S5MB25 [UniProt]
Representative
676qm
Source
UniProt (cluster: phalp2_23405)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKMYKRMAKSISYSPTKRNRKDVKYIVIHYTGNSNDSAKNNADYYATGNTRLAGAHFFVDKQGNIARSITLNRTAWAVGGGKYNDCKTTGGGGYYGKCTNFNSVSIELCSSTDKEAYSKEQAAAVKRLIKYIRKYCPNANAVIRHLDVNGKHCPAPMMNEKVWKKFKKAIGE
Physico‐chemical
properties
protein length:172 AA
molecular weight:19255,8 Da
isoelectric point:9,82
hydropathy:-0,70
Representative Protein Details
Accession
676qm
Protein name
676qm
Sequence length
150 AA
Molecular weight
17031,35560 Da
Isoelectric point
9,71940
Sequence
MEIKKRRAKSISYGSKRSLKGIKYIVIHFTGNKGDTAKNNADFYATGNTREAGAHFFVDKKSEIWESVPMEYTAWAVGHFFTRKNGAASYYKKCTNDNSVSIELCDCKKGVSWEQMLAVRELVQYIQKRCPNAKLSLDIGMSTAKRVQSR
Other Proteins in cluster: phalp2_23405
Total (incl. this protein): 129 Avg length: 170,1 Avg pI: 9,78
Protein ID Length (AA) pI
676qm 150 9,71940
13vz0 176 9,14428
1ks7y 172 9,93711
1ktVx 168 9,96863
21m6V 175 9,18392
21moc 175 9,26548
21ogJ 175 9,34335
23QHM 172 9,68884
23aHX 175 9,25826
23aij 168 9,79979
23sVs 169 10,09364
24EbE 173 9,90055
2t6I 160 9,92286
3B3SP 172 9,92002
3ITnx 172 9,76272
3TKOg 178 9,42736
3TOp4 167 9,55720
3TPdr 167 9,98643
3TRaL 178 9,57628
3VGoM 168 9,72249
3VHSr 172 9,87999
3VHZ8 174 9,98830
3VI5B 169 9,49434
3VLv3 180 9,37765
3WA8H 169 9,63359
3WGZ5 171 9,81823
3WJLe 169 9,81604
3WM65 172 9,57563
3WNdp 174 9,83750
3Wyi7 182 9,67930
3WzEm 171 9,94336
3ieOu 233 9,88502
3lSdB 171 9,92918
3lbSo 168 9,96818
3lh1q 174 9,94845
3lmkI 173 9,88515
3nxtV 172 9,77523
3shmM 172 9,82397
3tcCx 167 10,07069
3tzBA 173 9,85968
3ukp3 171 9,80559
3vDJp 171 9,85375
3vT9X 172 9,76266
3wMDz 172 9,84144
3wj9Y 169 9,81384
419Av 177 9,22254
41e9C 184 9,91396
41obj 174 10,01370
41pRL 173 9,26967
4L7p0 183 9,74867
4VOut 160 9,96908
5JXot 174 9,99126
5KE7k 172 9,84692
5M1Rl 169 9,79360
5MJIL 168 9,67060
5MiIO 179 9,88579
5OMew 172 9,89869
5P2df 165 9,88115
5PE6F 160 9,91828
5QgK0 172 9,78709
5QllE 134 9,88051
5RLJm 174 10,01254
5RrGy 165 9,76891
5Tb3B 168 9,83067
5UYnU 168 9,62760
5UfWO 165 9,89482
5UlGE 168 9,74635
5WTlC 174 9,90520
5X29P 172 9,75511
5YP7D 174 9,97063
5YT42 172 9,80804
5ZVOF 172 9,73055
614Yn 172 9,91964
61pon 172 9,85968
62qnu 168 9,76382
62sHA 149 9,88366
64xB5 169 9,71269
66K9e 160 9,86671
66U1v 168 9,76382
66xRC 172 9,82364
66y8A 167 9,60709
67yC4 172 9,76272
68iBx 174 10,07662
6Z8hI 172 9,76266
6a6Hn 183 9,87161
6aZ3E 174 9,99088
6bp62 174 9,96870
6bwmv 172 9,89907
6d0gL 152 9,79173
6ec2p 172 9,78561
6g9Dz 172 9,84943
6gazu 169 9,97411
6giZL 172 9,86368
6hL32 161 9,83892
6hiYn 170 9,73087
6ieMK 171 9,92918
6jS6h 172 9,76266
6ja5O 172 9,76272
6ke65 168 9,76382
6mt0x 169 9,90004
6n8vf 174 10,01254
6nIxc 169 9,89404
6nKTJ 174 9,93214
6o7PB 161 9,86046
6p9Bj 174 9,99126
6qs9o 170 9,73087
6r7DB 172 9,75511
6rB88 174 9,99088
6srf0 171 9,92918
6t31u 172 9,82364
6v0Pt 175 8,12123
6vYH9 125 9,85143
6vYVr 160 9,96908
6weaD 170 9,71353
6wt2y 172 9,84144
7Xuwn 160 9,89566
7wb6x 171 8,64574
82Goc 177 9,57905
834Gk 169 9,62224
85yt8 160 9,94555
8cEsy 168 9,73197
8eYJi 172 9,85968
8lb6U 174 9,97018
8oT8r 173 9,76852
8okPn 172 9,79708
8tJE6 124 9,60065
DwWI 168 9,94472
Ouw6 168 9,89527
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_38675
83KqQ
 128 56,2% 135 9.935E-48
2 phalp2_38069
6uYUB
 10 45,7% 107 3.051E-29
3 phalp2_14283
3qOze
 23 44,0% 93 1.107E-22
4 phalp2_20835
6rXam
 1 41,4% 94 2.847E-19
5 phalp2_30401
4NIg7
 17 45,7% 105 3.501E-18
6 phalp2_11551
100RI
 7 25,5% 141 1.679E-17
7 phalp2_24292
3k9WX
 42 33,0% 130 1.463E-13
8 phalp2_31879
4GdWf
 8 31,7% 104 2.730E-13
9 phalp2_25288
86peP
 187 30,2% 119 1.150E-11
10 phalp2_35735
47Q1t
 51 31,7% 145 2.260E-09

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 676qm (150 AA)
Member sequence: A0A8S5MB25 (172 AA)
1 150 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Myoviridae sp. ctNQr16
[NCBI] 		2826644 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014863 [NCBI]
CDS location
range 27231 -> 27749
strand +
CDS
ATGAAGATGTACAAGAGAATGGCTAAATCAATCAGCTATTCACCGACCAAAAGAAACAGAAAAGATGTAAAATACATAGTTATTCATTACACTGGAAATAGCAACGATTCGGCAAAAAACAATGCTGATTATTACGCTACAGGAAACACAAGACTTGCCGGAGCGCATTTCTTTGTGGATAAGCAGGGAAATATAGCCAGGAGCATCACTCTGAACCGAACGGCATGGGCAGTCGGAGGTGGAAAATACAATGATTGTAAAACTACAGGTGGTGGCGGTTATTACGGCAAATGTACTAACTTTAATTCGGTATCAATTGAATTATGCAGCAGTACGGACAAAGAAGCTTATTCAAAAGAGCAGGCAGCAGCAGTTAAGCGATTAATCAAATACATTCGCAAATACTGCCCTAATGCAAATGCAGTTATACGTCACTTGGATGTTAACGGAAAGCACTGCCCGGCTCCGATGATGAACGAAAAAGTATGGAAGAAATTTAAAAAGGCAATTGGCGAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (676qm) rather than this protein.
PDB ID
676qm
Method AlphaFoldv2
Resolution 85.48
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50