Protein

Protein accession
A0A8S5M6K9 [UniProt]
Representative
5oJrT
Source
UniProt (cluster: phalp2_16334)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSITWIGSPNHYAGRRGYRVTRITLHIMAGWLAGTDNIFQRASYQASSTYGIGGNGETHQYVAETDAAWADGSYTSNCQTISIEHQGGLDFIPCTQACLDASARLCADIARRYGFGKLERGRNIFLHRDVPPYAHPACPDLCPNGLNWRYIINKANQINGYGDIDMATAAEIWGYNYNKSALGGNMYNAINYELPGRISDVKKAVTALQATVAAQQRQIDKLTTALGSNPEDIADRTAKAVSDKIDKLVITMIAQEKDTAK
Physico‐chemical
properties
protein length:261 AA
molecular weight:28601,8 Da
isoelectric point:7,62
hydropathy:-0,36
Representative Protein Details
Accession
5oJrT
Protein name
5oJrT
Sequence length
248 AA
Molecular weight
27023,92550 Da
Isoelectric point
6,43067
Sequence
MVTVTQRPSPNHYAGRQGQRVAYITLHVMAGGLPGTDATFAKSSSRASSHYGVGADGTVSQYVSELDGSWADGSRISNLRSISIEHAGGLAGYVNTDACVSASAQLCADIARRYGWGRLSHGQNVFLHREIPPHTHPDCPDQCPNPLRWQEILTQANAILDNTSTPTDSQNGDFLMGLSQYDQELIRNAVVDINNRTDRLEKVVNAMALRLGPMPGYPFDWLPAISNNVVGLDRQVKQILNKLDKPTT
Other Proteins in cluster: phalp2_16334
Total (incl. this protein): 15 Avg length: 259,8 Avg pI: 5,92
Protein ID Length (AA) pI
5oJrT 248 6,43067
1NVsr 237 7,72171
5HNm8 248 6,21753
5W3Ha 270 4,88886
5oMI0 248 6,22082
61y8n 269 5,23910
62kCR 269 5,23950
65GNS 269 5,53495
690gL 270 4,76421
6AJlb 248 6,69827
6AJtr 248 6,43209
6fFek 269 5,06711
6s9WO 273 5,37364
89wzL 270 5,34584
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_32194
6VJSL
 13 39,5% 162 5.745E-36
2 phalp2_7058
8iEHQ
 9 37,7% 180 8.344E-34
3 phalp2_28639
8jnfC
 92 30,6% 202 1.309E-27
4 phalp2_25198
21qwy
 1 39,2% 158 5.329E-26
5 phalp2_14594
5ioYm
 94 29,8% 248 1.366E-21
6 phalp2_39194
3fUf1
 5 27,5% 174 3.843E-18
7 phalp2_33971
40cAJ
 14 30,5% 180 1.758E-17
8 phalp2_2329
4MdvC
 2 26,6% 214 2.381E-17
9 phalp2_11760
45aqa
 242 31,4% 159 3.226E-17
10 phalp2_35843
4HXSt
 1 27,5% 160 4.370E-17

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 5oJrT (248 AA)
Member sequence: A0A8S5M6K9 (261 AA)
1 248 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctETQ12
[NCBI] 		2826206 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014837 [NCBI]
CDS location
range 8477 -> 9262
strand +
CDS
ATGAGCATCACATGGATAGGCAGCCCCAACCACTACGCGGGGCGAAGGGGATACCGCGTCACACGCATCACCCTTCACATCATGGCCGGCTGGCTCGCCGGCACCGACAACATCTTCCAGCGCGCCTCATACCAGGCATCAAGCACCTACGGCATCGGCGGCAACGGGGAGACCCACCAGTACGTCGCCGAGACGGATGCCGCATGGGCGGACGGCAGTTACACAAGCAACTGCCAGACCATCAGCATCGAGCACCAGGGCGGACTCGACTTCATCCCATGCACCCAGGCATGCCTCGACGCCAGCGCCCGCCTATGCGCGGACATCGCCCGCCGGTACGGGTTCGGCAAGCTGGAACGCGGCAGGAACATATTCCTGCACCGGGACGTGCCTCCCTACGCGCACCCGGCCTGCCCGGACCTGTGCCCGAACGGGCTCAACTGGCGGTACATCATCAACAAAGCAAACCAAATCAACGGATACGGAGACATCGACATGGCAACAGCAGCCGAAATATGGGGATACAACTACAACAAGAGCGCGTTGGGCGGCAACATGTACAACGCCATCAACTACGAACTGCCCGGCCGTATCAGCGACGTGAAGAAAGCCGTCACTGCCCTGCAGGCAACCGTCGCGGCCCAGCAGCGGCAGATCGACAAGCTCACCACGGCGCTCGGCAGCAACCCCGAGGACATCGCCGACAGGACCGCCAAGGCCGTCAGCGACAAAATCGACAAACTCGTCATCACCATGATCGCACAGGAGAAGGACACCGCCAAATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (5oJrT) rather than this protein.
PDB ID
5oJrT
Method AlphaFoldv2
Resolution 84.60
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50