Protein

Protein accession
A0A8S5M561 [UniProt]
Representative
64hKp
Source
UniProt (cluster: phalp2_5925)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSYTLKTDLANRANYGRQRNTNKIKYLVFHATSNDGDTDESNARYFRTHVVKASAHAFIDDNSITISVPANYVAYSVGGKRYSDYNRTGGASMYGKITNTNSYNIEMCDCNKNGKYNFTEKTLENAVAYGKYIMNLYNIPITNVYMHFDVNGKHCPIQWWNKLEEWNKFKQRLRNTNVSTTVAQEMLYTKTQFIKDVQKAIGAGVDGKAGRETLSKTVTVSVTTNRKHAVVKPIQKYLNSKGFNCGTVDGCTGSKFDAAVKAYQRANGCIVDGVITAKGKTWKKLLGLA
Physico‐chemical
properties
protein length:289 AA
molecular weight:32372,3 Da
isoelectric point:9,59
hydropathy:-0,55
Representative Protein Details
Accession
64hKp
Protein name
64hKp
Sequence length
308 AA
Molecular weight
34630,99560 Da
Isoelectric point
9,63501
Sequence
MESIVRSLLLPYLSRKEKNMSYTLKTDLANRANYGRQRNTNKIKYLVFHATSNDGDTDESNARYFRTHVVKASAHAFIDDNSITISVPANYVAYSVGGKRYSDYNRTGGASMYGKITNTNSYNIEMCDCNKNGKYNFTEKTLENAVAYGKYIMNLYNIPITNVYMHFDVNGKHCPIQWWNKLEEWNKFKQRLRNTNVSTTVAQEMLYTKTQFIKDVQKAIGAGVDGKAGRETLSKTVTVSVTTNRKHAVVKPIQKYLNSKGFNCGTVDGCTGSKFDAAVKAYQRANGCIVDGVITAKGKTWKKLLGLA
Other Proteins in cluster: phalp2_5925
Total (incl. this protein): 120 Avg length: 316,8 Avg pI: 8,45
Protein ID Length (AA) pI
64hKp 308 9,63501
1FvOG 307 7,60053
1cMpe 307 8,40044
1jUko 328 8,65793
1kidx 322 8,01859
21EtR 300 8,87615
23HAH 300 9,09689
24nLd 305 8,11813
2c11u 301 9,22467
2o5lm 301 9,27476
3B2GG 326 8,01228
3B5lg 326 7,16515
3Q1Y6 301 9,04267
3TKwP 266 8,73890
3dQxr 266 9,68207
3jLT9 326 8,50062
3k7KS 328 7,97611
3kY2g 326 8,30915
3lDFm 289 9,38242
3lMXy 326 8,47232
3lRkH 326 8,49527
3lSET 326 7,19391
3lSvV 326 8,28981
3lcXL 322 8,62163
3mv6v 326 8,62163
3nJf9 276 8,99748
3pQOl 328 7,13161
3pTE2 326 8,47284
3qPzu 311 8,05586
3s0Z9 326 8,43989
3sPmM 322 8,61622
3sxK0 322 8,47703
3thFO 326 8,29091
3tmqI 289 9,50388
3uNEL 326 8,75102
3ukCl 326 7,60525
3vB37 326 8,04748
3vVHG 326 8,62163
3w0tA 326 6,88328
3waqX 328 6,84963
3wdHf 318 7,62793
3wgCB 326 8,63369
3wmRP 374 9,26051
3wzlL 326 8,47703
3xLT2 346 6,66252
3ys9G 326 8,72439
41nDX 259 8,78641
5KAJh 326 8,02201
5MGf0 322 8,28272
5MIq2 322 8,76269
5MVHN 322 8,50565
5NRpo 322 8,28788
5OPte 326 8,47651
5Ohey 300 9,07716
5QNnX 328 7,96367
5R2qQ 326 7,56785
5TFnX 326 8,29400
5URJS 326 8,31354
5UYgl 326 8,65019
5VLvt 328 8,22264
5WUxg 326 8,02208
5Wlir 328 7,98874
5XQ0G 326 8,02897
5XrsT 326 8,00125
5YeLW 326 8,72949
5ZtOU 326 8,28981
60GND 308 9,63501
60TdL 306 8,32243
61kPA 298 9,04725
61yoW 326 8,47696
62EsE 322 7,62793
64g1g 326 8,29407
65OpS 326 8,29091
65btI 326 8,30915
68E5G 326 8,00763
695V5 289 9,40885
69iaN 326 8,32243
6bQYm 300 9,16226
6bbC2 326 8,73490
6c3xK 326 8,28878
6cHIu 289 9,45463
6dXWZ 322 8,28272
6hJxa 300 9,28765
6i3xy 326 8,47239
6jkCF 326 7,16157
6kKqu 322 8,05115
6kydt 328 7,56467
6lcYN 326 8,64439
6m9HO 326 8,02201
6o2Jn 289 9,45463
6oOTK 326 8,74090
6otis 322 8,46775
6pPfO 300 9,23943
6qVCF 317 8,01859
6qzlQ 326 8,61647
6uwJr 298 9,52735
6vPhM 322 7,19419
6vkbH 326 8,61647
7OH2s 326 8,05025
7pEZV 265 9,84337
7rR05 237 9,85072
7rWQu 375 9,26045
7sIvZ 326 8,84308
7sIzV 326 8,73522
7sxye 326 8,28459
7t45L 326 8,61112
7tzUP 328 8,22141
7tzYM 326 8,61647
7vHJz 326 8,47232
7vVmN 328 8,22618
7vVnN 328 6,81871
7vWXa 237 9,85072
87V4n 326 8,51023
8fCER 328 8,58424
8jDXd 326 8,46878
8m5JY 326 8,47703
8oEQJ 289 9,46584
8pBdc 328 7,13212
a1y4 305 7,60053
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_38692
MSLD
 8 41,8% 320 2.789E-94
2 phalp2_36746
6AI9B
 16 29,2% 328 6.295E-61
3 phalp2_17898
EGF4
 130 31,0% 312 2.196E-60
4 phalp2_26828
3TOBT
 44 37,8% 246 3.771E-54
5 phalp2_32877
3ejEW
 46 30,0% 339 5.518E-52
6 phalp2_31053
1GV4E
 13 32,1% 249 7.534E-52
7 phalp2_7264
3WBGP
 11 27,0% 281 1.080E-44
8 phalp2_38909
238Ru
 1 29,8% 261 4.463E-43
9 phalp2_6450
8Jtqs
 137 26,6% 296 2.593E-39
10 phalp2_10688
2YkiQ
 5 29,5% 291 6.552E-39

Domains

Domains [InterPro]
Disordered region
Ami2
PG_1
Representative sequence (used for alignment): 64hKp (308 AA)
Member sequence: A0A8S5M561 (289 AA)
1 308 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctPJC19
[NCBI] 		2826321 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014824 [NCBI]
CDS location
range 17254 -> 18123
strand +
CDS
ATGTCTTATACTTTAAAAACAGATTTAGCAAATAGGGCTAATTATGGTAGACAGAGAAACACTAATAAAATCAAATATCTTGTCTTTCACGCAACATCAAATGACGGTGATACAGATGAATCAAATGCGAGATATTTTAGAACTCATGTTGTAAAAGCTTCTGCCCATGCATTTATAGATGACAATTCAATTACTATATCTGTTCCTGCAAACTATGTAGCTTATTCAGTTGGTGGAAAACGTTATTCTGATTACAACCGTACAGGTGGAGCTTCTATGTATGGTAAAATCACTAATACAAATTCTTACAATATTGAAATGTGTGACTGTAACAAAAATGGCAAATATAATTTCACAGAAAAAACATTAGAAAACGCAGTTGCATATGGTAAATATATTATGAATTTATATAACATCCCGATTACAAATGTTTATATGCATTTTGATGTTAATGGAAAACATTGCCCTATTCAGTGGTGGAATAAACTAGAAGAATGGAACAAATTCAAACAGCGTTTAAGAAACACAAATGTTTCTACTACTGTAGCACAGGAAATGCTTTATACAAAAACACAGTTCATTAAAGATGTTCAGAAAGCTATTGGTGCAGGGGTTGACGGAAAAGCCGGTAGAGAAACATTATCAAAAACTGTAACAGTATCTGTAACTACAAACAGAAAACATGCTGTAGTTAAACCAATCCAGAAATATTTAAATTCAAAAGGATTTAATTGTGGCACGGTAGATGGTTGCACAGGTTCAAAATTTGATGCTGCCGTAAAAGCATATCAGAGAGCAAATGGATGTATTGTTGATGGTGTGATCACGGCAAAAGGTAAAACATGGAAAAAATTGCTTGGGTTAGCCTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (64hKp) rather than this protein.
PDB ID
64hKp
Method AlphaFoldv2
Resolution 87.76
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50