Protein

Protein accession
A0A8S5LKG2 [UniProt]
Representative
MSLD
Source
UniProt (cluster: phalp2_38692)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MRSSSRSISARSRRRIRTPLTRGRKAMDIRKYPANAGNVGGKRTASGIRYIVIHYTGNDGDTAANNAKYYAGNVVKTSAHYFVDANEIVQSVDDLRIAWAVGGNKYPSCAQTGGGTMYGKCKNANSISIELCDAVKNGVYAPDAKTVSQALELTKALMKKYNIPASNVIRHFDVTGKLCPAYWSGKENTGKWEKEFHGKLTAPDYRAQLQKRAGLTDGTMDYLEKYQYGDDLIRKLAVMK
Physico‐chemical
properties
protein length:240 AA
molecular weight:26505,9 Da
isoelectric point:9,76
hydropathy:-0,58
Representative Protein Details
Accession
MSLD
Protein name
MSLD
Sequence length
463 AA
Molecular weight
52392,79320 Da
Isoelectric point
9,10192
Sequence
MSFIEDVYKIMRDVVPKYGIKCYSAPLAQFVLESANGTSELAINAHNYAGLKYRANRCPTSNGIYNKVGSEQDAATGQYISSNMNWFKFPDMKSGVIGYFDFINISNYANLNGITDPKEYLETIKADKYCTSINYVQNCMNVIQKYNLTKYDKKGENNMGYIMKTNLANRANYGNMRSTSKIKYIVIHYTANDGDSDEANGNYFHNRVVKASAHYFVDDDSITQSVPDNYVAYSVGDKRYSNYKTTGGAKFYGICTNTNSISIEMCDTKKDDIHNVTKTTIENTIALVKILMKKYNVTIDRVIRHFDISGKPCPSYYVNESEWAIFKNRISPSSATTSTSPSFYMYGAEPKSIITGNPNQFTQIVQNIKTVLNKDYGLKFTINGVADDLLLTNLGNIVMSTASYNKNLTYCLQQLFKWWNVNITIDGLYGSGTKASVMNFQKIWNIAQTGRTTKKFWYKILGK
Other Proteins in cluster: phalp2_38692
Total (incl. this protein): 8 Avg length: 359,3 Avg pI: 8,91
Protein ID Length (AA) pI
MSLD 463 9,10192
23Qq5 417 7,53142
4Laao 414 8,40270
A0A8S5NJ65 327 9,48847
A0A8S5RNW3 463 9,05518
A0A8S5TC09 295 9,04545
A0A8S5UY55 255 8,90387
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_32877
3ejEW
 46 44,8% 486 7.811E-133
2 phalp2_5925
64hKp
 120 44,6% 329 2.182E-97
3 phalp2_36746
6AI9B
 16 33,7% 329 2.650E-61
4 phalp2_17898
EGF4
 130 33,6% 309 1.438E-59
5 phalp2_20926
7aLO0
 122 34,3% 320 5.688E-47
6 phalp2_21762
3TRfz
 37 21,9% 515 2.048E-40
7 phalp2_28439
1KvxT
 4 27,0% 348 2.261E-39
8 phalp2_21648
2Vbrp
 10 24,2% 450 8.974E-37
9 phalp2_31221
84SiK
 5 26,9% 326 7.931E-30
10 phalp2_6450
8Jtqs
 137 29,1% 302 3.583E-28

Domains

Domains [InterPro]
GLUCO
Ami2
Unannotated
Representative sequence (used for alignment): MSLD (463 AA)
Member sequence: A0A8S5LKG2 (240 AA)
1 463 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF01832

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctnhN1
[NCBI] 		2827589 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015865 [NCBI]
CDS location
range 22776 -> 23498
strand +
CDS
ATGCGGTCATCATCGCGTTCTATTTCGGCACGCAGTCGCAGAAGAATCAGGACGCCATTGACAAGGGGGCGTAAGGCAATGGACATTCGCAAATATCCCGCGAACGCCGGGAACGTCGGCGGCAAGCGCACGGCGAGCGGTATCCGCTACATCGTGATCCACTACACCGGCAACGATGGCGACACGGCGGCGAATAACGCGAAGTACTACGCGGGCAACGTCGTGAAGACCAGCGCGCACTACTTCGTCGATGCAAACGAGATCGTGCAGAGCGTGGACGACCTGCGCATCGCGTGGGCGGTCGGCGGCAACAAGTATCCGAGCTGCGCGCAGACTGGCGGCGGGACGATGTACGGCAAGTGTAAGAACGCCAACAGCATCAGCATTGAACTGTGTGACGCGGTCAAGAACGGCGTATACGCGCCGGACGCGAAGACCGTCTCGCAGGCACTTGAGCTGACGAAAGCTCTGATGAAGAAGTACAACATCCCCGCGAGCAACGTCATCCGCCATTTCGACGTGACGGGCAAGCTGTGCCCCGCGTACTGGTCCGGCAAGGAGAACACGGGCAAGTGGGAAAAGGAATTCCACGGCAAGCTGACGGCGCCCGATTACCGCGCGCAGCTGCAAAAGCGCGCGGGGCTGACGGACGGCACAATGGATTACCTTGAAAAATATCAGTACGGCGATGACCTCATTAGAAAACTCGCCGTAATGAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (MSLD) rather than this protein.
PDB ID
MSLD
Method AlphaFoldv2
Resolution 88.35
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50