Protein

Protein accession
A0A8S5LJ22 [UniProt]
Representative
3tObF
Source
UniProt (cluster: phalp2_20140)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MLTINKEFLKSYKCYKGKNNPKYIVNHETDNYSEGAGARNHAKAQFNGNLGNASVHFYVDDKEIYQCLELSDGPWSVGDANYGVITNWNNISIEICVNPDSDYNTARKNAAELNRYLLDKYGWGMDRVKRHYDATYKTCPRKMIQNPNLWKEFIQWINTGDTSIEVDHNKPIYVATIEQSKNFIGNRAKDVQAKLISLGYDLGSWGANGIWGEYSYNALLKFQKDNGLNPDGYCGPATTAKLNELCENKGESRLLKVMKNKVVKLGIEGNHVRMLQSSLTMLGYNVNGIDGHCGNGCVAAIKAYQRDNGLSVDGSCGPATWTSILTK
Physico‐chemical
properties
protein length:327 AA
molecular weight:36583,8 Da
isoelectric point:8,42
hydropathy:-0,58
Representative Protein Details
Accession
3tObF
Protein name
3tObF
Sequence length
433 AA
Molecular weight
48022,95850 Da
Isoelectric point
8,67759
Sequence
MLKIMGQATATVAQMQSYIKKVNPQVPNSVIKMIPLYITEGAAEGVRGDIAFAQSCLETGNFTFTGSAVTLSQNNFAGIGVTQTGMKGNSWESPQLGIRAQIQHLKAYACDQRLLQTCVDPRFTYVTRETAPYVEWLGIQENPHHYGWAADKDYGQKIIQILNSILSIQINETDQKGNDMDIIKMISKKNCYIGQNKPAYVVIHETDNWSKGANAKCHAQAMKNGNLAGTVHYYVDSNDIYQTLDHSDGAWAVGDGQGKYGITNRNSINIEICVNPETDYYTAVDKAEQLTALLLKQYGWSTDRLKRHYDASRKHCPRRILDEGLWPKFVEKTTAYMIGASGNASTNTSTTKGAYMFTPNTVKAGDKNTSVLLLQEILRARGFKGKNGKALKLTWSADTNTIYALKAYQESRKEVLEVDGICGPATWKDLIAI
Other Proteins in cluster: phalp2_20140
Total (incl. this protein): 100 Avg length: 452,2 Avg pI: 9,16
Protein ID Length (AA) pI
3tObF 433 8,67759
14ut 444 9,54959
1FYrv 433 9,53160
1kc4L 476 7,15895
1o3t7 420 9,73448
1o6zy 482 8,91283
1o7yN 422 8,90439
1obgC 482 9,01682
3kNYz 477 9,61870
3kjRr 473 9,08567
3kopH 482 8,85630
3kxeE 482 9,02643
3lG5Q 430 9,05460
3lRXu 482 8,91277
3lTtV 444 9,27953
3lUKR 480 9,15833
3pB2m 482 8,91277
3pNIq 462 9,12242
3pObb 471 8,42881
3pkAL 473 8,89240
3qmfC 482 8,91715
3qrg4 477 9,11269
3qvDh 433 9,54830
3rm3q 482 8,78590
3rmVz 473 8,98188
3sDeL 473 9,56100
3svcN 455 9,01650
3svtv 430 9,10805
3tknX 482 8,91735
3tlnl 430 9,19914
3u0vi 433 9,42181
3u1K8 433 9,48325
3uq4x 482 9,02159
3vJg7 473 9,33807
3wL7B 480 9,06434
3wY4x 446 9,51774
3wYYu 477 9,47674
3xs9q 477 9,41382
5K80B 480 9,05924
5LbrT 439 9,23427
5N3BT 434 9,44386
5OFBd 426 9,32711
5PTpU 433 9,57950
5PnoB 423 9,00851
5RcO6 475 8,68545
5RhPf 494 9,65699
5T3bm 474 9,39177
5TZjv 477 9,54572
5TdSx 473 9,02798
5TzGs 463 8,74122
5UmpT 477 9,47332
5V1Wa 477 9,56087
5WoIc 442 9,51774
5Ym0F 433 9,47648
5YqOo 509 8,73226
5Yxjy 494 9,65699
5Yy74 473 8,68146
5Z7bX 433 9,30699
60jH5 477 9,26986
61PAa 442 9,43284
61ibM 472 8,34796
66952 442 9,45095
669Br 283 9,29984
69t0W 471 8,29420
69y4q 429 9,17084
6aAol 433 9,54031
6atSM 493 9,51626
6bgCo 442 9,49266
6en8n 474 9,24046
6fZcD 441 9,45282
6h7sC 480 9,20713
6oXcR 312 9,34252
6px4R 298 9,41524
6uCMV 474 9,22267
6uf65 473 9,06085
6vSLz 428 9,25065
6vSbE 433 9,54817
7Bfa1 442 9,34052
7X31D 473 9,56893
7X924 392 9,64023
7Xv04 442 9,40415
7gZtR 481 9,58227
7t3xz 431 6,73101
7w5cX 509 8,18383
7wHUo 433 9,52425
7wSAp 424 9,11881
7wSO5 429 9,42413
7xuaO 473 9,13486
7ymlG 482 8,97369
7yryG 477 9,46887
80aYP 477 9,55326
85fWy 433 9,45714
8dhbG 448 8,40463
8om3z 477 9,34142
8qlRk 457 9,08232
8sire 348 9,14627
CNCp 473 8,91271
OGhs 440 9,49266
A0A8S5MUF9 477 9,38893
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_23855
1rrTr
 15 30,7% 354 1.222E-39
2 phalp2_32877
3ejEW
 46 25,7% 381 4.715E-26
3 phalp2_38692
MSLD
 8 22,1% 316 1.240E-22

Domains

Domains [InterPro]
GLUCO
Ami2
Unannotated
Representative sequence (used for alignment): 3tObF (433 AA)
Member sequence: A0A8S5LJ22 (327 AA)
1 433 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF01832

Taxonomy

  Name Taxonomy ID Lineage
Phage Myoviridae sp. ct6F13
[NCBI] 		2827602 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015859 [NCBI]
CDS location
range 36124 -> 37107
strand +
CDS
ATGCTTACAATCAATAAAGAATTTTTAAAATCCTATAAGTGCTATAAGGGAAAAAATAATCCTAAATATATAGTTAACCATGAAACAGATAACTATAGTGAAGGAGCAGGAGCAAGAAATCATGCTAAAGCTCAATTTAATGGGAATTTAGGAAATGCTTCTGTTCATTTTTATGTAGATGATAAAGAAATCTATCAATGTTTAGAGTTAAGTGATGGTCCATGGTCCGTAGGAGATGCAAATTACGGAGTAATTACAAATTGGAATAATATCAGCATAGAAATATGTGTTAATCCTGATTCAGATTACAATACAGCTAGAAAGAATGCAGCTGAATTAAATAGGTACTTATTAGACAAATATGGCTGGGGAATGGACAGAGTAAAAAGGCATTATGATGCTACTTATAAGACATGCCCCAGAAAGATGATTCAAAACCCCAATCTATGGAAAGAGTTCATACAGTGGATTAATACTGGTGATACTTCTATAGAGGTAGATCATAACAAACCTATTTATGTTGCAACTATAGAACAATCTAAAAACTTTATAGGAAATAGAGCAAAAGATGTACAAGCTAAACTTATAAGTCTAGGGTATGATCTAGGAAGCTGGGGTGCTAATGGTATATGGGGTGAGTATAGTTATAATGCTTTACTTAAGTTCCAAAAAGATAATGGATTAAATCCAGATGGGTATTGTGGACCTGCAACAACAGCCAAGCTAAATGAACTATGTGAAAATAAGGGGGAGTCAAGATTGTTAAAGGTAATGAAGAATAAGGTTGTTAAACTAGGTATTGAAGGAAATCACGTTAGGATGCTTCAATCTAGCTTAACAATGCTAGGTTATAATGTAAATGGAATAGATGGTCATTGTGGTAATGGATGTGTAGCTGCTATTAAAGCATATCAAAGAGATAATGGATTATCTGTAGATGGTTCTTGCGGGCCAGCAACTTGGACTAGTATTTTAACTAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (3tObF) rather than this protein.
PDB ID
3tObF
Method AlphaFoldv2
Resolution 88.30
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50