Protein

Protein accession
A0A8S5LF58 [UniProt]
Representative
83KqQ
Source
UniProt (cluster: phalp2_38675)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSYKFKEIWCNKGNFTEDNRKSSEIDTLVIHYTGNNGDTAENNGNYFKNNVVETSAHYFVDDTTVVRSVADKNIAWHAGDWNINCRSIGIEIAGSTSECKGKTLENVILLTQRLMRKYKISKERVIRHYDANGKICPGFWCGSSAKDKLWKEQFLNKLESNSESKEESKVE
Physico‐chemical
properties
protein length:171 AA
molecular weight:19571,7 Da
isoelectric point:7,62
hydropathy:-0,75
Representative Protein Details
Accession
83KqQ
Protein name
83KqQ
Sequence length
151 AA
Molecular weight
17036,85470 Da
Isoelectric point
6,51155
Sequence
MSYKFKEIWCNKGNFTESNRKSSEIDTLVIHYTGNNGDTAENNGNYFKNNVVETSAHYFVDDTTVVRSVADKNIAWHAGDWDINCRSIGIEIAGSTTECTGKTLENVILLAQRLMKKYNISKERVIRHYDANGKICPGFWCGSSAVSFHIE
Other Proteins in cluster: phalp2_38675
Total (incl. this protein): 128 Avg length: 198,5 Avg pI: 9,87
Protein ID Length (AA) pI
83KqQ 151 6,51155
13wdo 163 9,70670
1jHMe 166 9,96515
1jT3U 231 10,31141
1kc0o 137 9,20172
1qUgh 173 10,00815
21f06 248 9,80269
21phn 242 10,05347
21w7t 245 9,59839
21wa8 250 9,45108
235rf 246 9,51490
23GSH 270 9,34619
23KDa 262 9,73036
23Lw1 257 9,85807
23Nm7 231 9,84872
23c3Q 240 10,19582
23dVB 257 9,75885
23wbm 251 9,83267
24KJP 245 9,67459
24q03 169 9,72804
2mc3G 234 10,03149
3A4Gl 173 9,98050
3A4U9 173 10,06334
3GQ9c 166 10,14128
3LQiD 166 10,18299
3TRMC 237 10,18260
3VGxp 171 9,67111
3WDHm 237 10,00525
3WEqW 170 9,73171
3WH4u 172 9,76233
3Wyh8 172 9,96232
3ZQhS 257 10,07481
3gLVx 259 9,72243
3j0Bl 181 9,13847
3kTG7 243 9,93040
3l0TC 234 9,92376
3mtXx 170 9,92757
3rmdM 234 9,94156
3tQkJ 166 10,26132
3taaG 234 9,97779
3ymfP 166 9,83435
40cHd 239 9,91197
41bM4 196 9,81778
41fBM 177 9,83654
41i9Y 194 9,78567
41iFH 173 9,61554
4kkcu 236 9,92518
5KHti 230 9,93866
5KOLr 230 10,07526
5Labl 166 10,01827
5MTCB 166 10,12768
5OGR2 166 10,15153
5QptN 166 10,07746
5RP6w 234 9,94027
5RqYn 166 10,18299
5SJ1e 230 10,03633
5SMcn 230 10,03394
5T0oX 166 10,12768
5USnc 230 9,90043
5UstP 230 10,03504
5XHMv 166 10,04748
5YH9H 189 9,79618
5Z19f 166 9,99681
5ZX79 230 9,82371
604V2 230 10,05412
60dfL 166 10,20485
63dbt 194 9,82061
649FM 166 10,12839
64npU 166 10,01879
66Qqm 230 9,89591
67f1I 230 10,11678
684nA 174 9,87619
68tiT 168 10,01769
69SSq 230 9,86226
6YjRA 166 10,16068
6aXqz 230 9,99487
6bJjN 230 10,03394
6bSUi 234 9,97643
6eKaY 230 10,05618
6f8DJ 166 9,99249
6frzw 166 10,04148
6hQFB 234 9,95987
6hh7k 230 9,99835
6k57J 189 9,81900
6kAi8 166 10,07391
6kntS 230 9,86226
6lcZg 166 10,09699
6lkOz 194 9,93569
6sgDf 196 9,95645
6v6xG 239 10,16004
6vOgv 166 10,18299
71tVa 238 10,15624
7VqsI 163 10,02717
7Y6Ml 234 9,99597
7Y6ww 174 9,94078
7YxCh 166 10,19356
7t17I 194 9,89939
7tRhb 196 9,75769
7ypbO 194 9,93859
82GAH 167 9,80031
82GWR 167 9,76298
84m8L 166 10,07391
84pRx 243 9,96547
85Oki 164 9,75782
86elI 172 9,82364
887d1 162 9,69722
887ma 173 9,75744
888Wq 160 10,09854
889Hb 163 10,00777
8br9w 166 9,62012
8cCeN 230 9,90017
8dlzW 230 9,90043
8fBnW 166 10,12071
8fHuJ 166 10,07681
8lUY2 159 9,88199
8lUle 166 10,02008
8mp3C 173 9,73655
8mpyi 164 9,74486
8pV8H 164 9,72875
8pZoO 182 9,86355
8qtm4 173 9,72765
8r5BO 166 10,04683
EFEr 189 9,80469
EJkz 236 9,87238
JO77 234 9,92376
oTMg 194 9,89469
oeXh 166 9,63391
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_29669
8sfID
 1005 45,7% 153 8.321E-49
2 phalp2_24299
3nIle
 36 47,0% 134 5.713E-45
3 phalp2_23405
676qm
 129 29,6% 135 2.517E-43
4 phalp2_1605
81mMl
 270 41,6% 149 1.382E-40
5 phalp2_15490
7YA8O
 2 39,6% 159 1.143E-38
6 phalp2_34832
3TNM2
 9 30,2% 152 1.039E-37
7 phalp2_3793
5X3JD
 59 43,0% 137 6.262E-36
8 phalp2_38069
6uYUB
 10 47,2% 110 7.997E-32
9 phalp2_14283
3qOze
 23 41,5% 118 6.580E-30
10 phalp2_9579
1njYk
 79 28,3% 155 3.178E-29

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 83KqQ (151 AA)
Member sequence: A0A8S5LF58 (171 AA)
1 151 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctABi4
[NCBI] 		2823566 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014705 [NCBI]
CDS location
range 1 -> 513
strand -
CDS
ATGTCTTATAAATTTAAAGAAATATGGTGCAACAAAGGTAATTTTACAGAGGACAACAGAAAATCTTCGGAAATTGATACACTTGTTATTCATTACACCGGCAACAACGGCGACACAGCAGAAAACAACGGTAACTACTTTAAGAATAATGTAGTTGAAACATCTGCACATTATTTTGTCGATGATACAACAGTTGTACGATCTGTCGCTGACAAAAATATTGCTTGGCACGCAGGTGACTGGAATATTAATTGTCGCTCAATCGGCATTGAGATTGCAGGCTCAACAAGTGAGTGCAAGGGAAAAACGCTTGAGAATGTAATTCTGCTTACTCAGCGACTTATGAGAAAGTACAAAATCAGTAAAGAGAGGGTGATTCGTCATTATGACGCAAACGGCAAAATCTGCCCAGGGTTTTGGTGCGGTTCATCAGCAAAGGACAAGCTGTGGAAAGAACAGTTTTTAAATAAACTTGAGAGTAATTCTGAAAGCAAAGAGGAATCAAAAGTTGAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (83KqQ) rather than this protein.
PDB ID
83KqQ
Method AlphaFoldv2
Resolution 93.26
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50