Protein

Protein accession
A0A8S5L6T8 [UniProt]
Representative
8cbIl
Source
UniProt (cluster: phalp2_868)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTNAAVTDVQWSPNYSSGRPYGDVDSITIHHWGVDGQSHQNVVNYLCREDGDSSAHYVASAGRVTQLVHDYDRAWHAGPGGNPRSIGIECRPEMTDGDVATVIGLIQAIRAEHGPLPIVGHRDWMSTDCPGRWYSHLSELSNGSGFGAVSSPAPVVDVNPYTGKWNKSDGQGELRCTGVFGMATIGRLQQVMGTTVDGVLDEDGSPAIERFQAFLNSVVPADTQIALNDAPALETDGILGPNTWRTFQYLVIAWHKEYLPAGWDFADWVDGEAGTATIGALQRALNNSTSGTGKLW
Physico‐chemical
properties
protein length:296 AA
molecular weight:31894,9 Da
isoelectric point:4,82
hydropathy:-0,32
Representative Protein Details
Accession
8cbIl
Protein name
8cbIl
Sequence length
172 AA
Molecular weight
18376,04460 Da
Isoelectric point
4,34917
Sequence
MNTDCPGRWYSHLAELSNESGFGAASAPQPAVDVNPYTGNWNATDGQGELRLTGNFGIATIGRFQQVMGTTIDGVLDDDGSPAVERFQAFLNSVVPADSQIALNGAPALDVDGIFGPNTWRTFQYLVLAWHKGYVPDGWDFGDWVDGVPGAATVKALQQALNNSRSGTGKLW
Other Proteins in cluster: phalp2_868
Total (incl. this protein): 6 Avg length: 179,8 Avg pI: 5,58
Protein ID Length (AA) pI
8cbIl 172 4,34917
2biUb 140 4,40385
8h9o8 169 4,24607
K61t 141 6,84037
ym0W 161 8,83154
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_12018
7MMHs
 20 25,5% 149 1.324E-06
2 phalp2_39082
2bOI7
 1 28,3% 113 3.285E-06
3 phalp2_1816
3ebwD
 3 25,5% 137 1.487E-05
4 phalp2_12804
8lvpW
 5 26,2% 137 1.647E-04
5 phalp2_28965
5TxsT
 1 25,3% 162 9.893E-04

Domains

Domains [InterPro]
Disordered region
Unannotated
Representative sequence (used for alignment): 8cbIl (172 AA)
Member sequence: A0A8S5L6T8 (296 AA)
1 172 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ct45W1
[NCBI] 		2823562 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014646 [NCBI]
CDS location
range 24688 -> 25578
strand -
CDS
ATGACCAATGCTGCTGTGACCGACGTCCAGTGGTCCCCCAACTACTCCAGCGGGCGCCCCTATGGCGACGTTGACTCCATCACGATCCACCACTGGGGTGTTGACGGCCAGTCTCACCAGAACGTGGTGAACTACCTCTGCCGCGAGGACGGCGACTCCAGCGCCCACTACGTCGCCAGCGCCGGCCGCGTGACCCAGCTGGTCCACGACTACGACCGCGCCTGGCATGCCGGCCCTGGTGGCAATCCCCGCTCCATCGGCATCGAGTGCCGGCCGGAGATGACCGATGGCGACGTGGCGACCGTGATCGGTCTGATCCAGGCGATCCGTGCAGAGCACGGCCCCCTGCCGATCGTGGGACACCGCGACTGGATGAGCACCGACTGCCCCGGCCGCTGGTACTCGCACCTGTCCGAGCTGTCCAACGGCTCAGGCTTCGGGGCGGTGTCGTCCCCGGCTCCGGTGGTGGACGTGAATCCCTACACGGGCAAGTGGAACAAGTCCGACGGCCAGGGTGAGCTCCGGTGCACCGGCGTCTTCGGCATGGCGACCATCGGCCGGCTTCAGCAGGTCATGGGCACCACCGTCGATGGCGTGCTCGACGAGGACGGCTCTCCCGCCATCGAGCGGTTCCAGGCGTTCCTCAACTCGGTCGTGCCGGCGGACACGCAGATCGCGCTGAACGACGCCCCGGCGCTGGAGACTGACGGCATCCTGGGGCCGAACACCTGGCGGACGTTCCAGTACCTCGTGATCGCCTGGCACAAGGAGTACCTGCCCGCCGGCTGGGACTTCGCCGACTGGGTTGACGGTGAGGCCGGCACCGCCACGATCGGGGCGCTCCAGCGCGCGCTGAACAACTCCACGTCGGGGACCGGCAAGCTCTGGTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0019867 outer membrane cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8cbIl) rather than this protein.
PDB ID
8cbIl
Method AlphaFoldv2
Resolution 86.59
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50