Protein

Protein accession
A0A8D9UHF0 [UniProt]
Representative
7kPAJ
Source
UniProt (cluster: phalp2_29147)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAFLSPDNVRYDNGVKICEKLIPDSAVWNRDYTEAGYTYRKGTQYKANRALSAINGVTIHNTGRIKVPSGTTMAEQYTRATYPNCNMGSVRVHYYVDENEAWQNLDEGEVGWHAADGNYGPGNSTTIAIEIIMDGTDAEYNRIAEDNGARLCAAILKRHGLDETAVYQHHDWYARKDCPVYIRPHWSAFLALVRQYLNDDTQVPSDYDKLVAELEDIKEKYRTEHASAQALRGRILAAVEQYDTAAYDKEG
Physico‐chemical
properties
protein length:251 AA
molecular weight:28385,1 Da
isoelectric point:5,28
hydropathy:-0,65
Representative Protein Details
Accession
7kPAJ
Protein name
7kPAJ
Sequence length
377 AA
Molecular weight
39999,88630 Da
Isoelectric point
8,96286
Sequence
MAFLTPDKTLTVSVGSETITIKQKITPDGTRAAKNICSYVKKGDYVKPNEKLNNGTGVPKGITVHNTGDIACASGTTPAEQYARATYPNGNMNGVAVHYWVWHNEIWQQLSDNERGWHAADGSSRRSSQRAGETIGGNLDTIAIECIGADASSEATLAKLVAYLLDKHGLSPSTDVYTHNYFYSKKYCPIYILPHWGSFLSTVKANYNAIQSAKAPAVIETPAPVQQAPTIIVGSTVRVKSGASTYTGGGLAGFVYERDHIVKEIKGDRAVITYGGVVVAAVKLSDLSLTNSATYTPAPAPAPAPTPEPVPVAPVATIEVGSTVRLNSGAKTYTGGRLASFVYKRDHIVKEIKGDRAVITYGGVVVAAVKLCDLTLA
Other Proteins in cluster: phalp2_29147
Total (incl. this protein): 3 Avg length: 329,7 Avg pI: 7,79
Protein ID Length (AA) pI
7kPAJ 377 8,96286
78G0D 361 9,13847
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_15172
oqn3
 103 39,4% 284 1.776E-64
2 phalp2_37067
101m6
 139 33,9% 289 2.406E-40
3 phalp2_4501
3nOos
 2 27,1% 365 5.847E-38
4 phalp2_1131
3cMm
 37 30,1% 348 1.022E-23
5 phalp2_29156
7qyOK
 47 26,6% 304 3.097E-12
6 phalp2_14834
7s5S0
 47 29,1% 278 2.271E-11

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Representative sequence (used for alignment): 7kPAJ (377 AA)
Member sequence: A0A8D9UHF0 (251 AA)
1 377 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Myoviridae sp. ctjz83
[NCBI] 		2826083 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014725 [NCBI]
CDS location
range 17600 -> 18355
strand +
CDS
ATGGCTTTCCTCTCTCCAGATAATGTACGCTATGATAACGGCGTAAAAATCTGTGAAAAGCTTATTCCTGATAGCGCCGTATGGAACCGAGACTATACCGAGGCCGGTTATACATACCGCAAAGGTACGCAGTACAAGGCAAACCGGGCGTTATCCGCCATTAACGGTGTGACTATTCACAATACTGGCCGGATTAAAGTCCCCAGCGGTACTACAATGGCCGAGCAGTACACCAGAGCAACTTATCCCAACTGCAACATGGGGTCTGTCCGTGTCCACTACTATGTGGACGAGAACGAAGCATGGCAGAACCTTGACGAGGGCGAGGTCGGCTGGCACGCGGCTGATGGAAACTACGGCCCCGGCAACAGCACTACCATCGCCATCGAGATCATCATGGACGGCACTGATGCCGAGTATAACCGGATTGCCGAAGATAACGGTGCAAGACTTTGCGCTGCTATTCTAAAACGGCACGGTTTGGATGAAACCGCAGTCTACCAGCACCATGACTGGTACGCAAGGAAAGACTGCCCTGTCTATATCAGACCGCACTGGAGCGCGTTTTTGGCGTTGGTGCGGCAGTATCTCAATGACGATACGCAGGTGCCGAGCGATTATGATAAGCTGGTCGCCGAGCTGGAAGACATCAAAGAGAAATACAGAACCGAACACGCCAGCGCGCAGGCGCTGCGTGGGAGAATTTTAGCCGCCGTGGAACAGTATGACACGGCGGCATATGACAAGGAGGGGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7kPAJ) rather than this protein.
PDB ID
7kPAJ
Method AlphaFoldv2
Resolution 87.49
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50