Protein

Protein accession
A0A6J7WLT7 [UniProt]
Representative
1hMzx
Source
UniProt (cluster: phalp2_29706)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGFKMKYEIKKQYLTIPSNRSSGKPMDKIVFLVAHDTGNPGSTAKNNVDYYERSRDEISASAHIFVDDKEIIECVPFLTDKPLKAWHVLYNVDNDNRLYNANANDSAGGFELCYGGKINLEESYKRYVWVMAYACHVYKLDPMAKITGHYILDPKRKTDPQLSLKLLNKTLNDLIKDVSKELKDCMS
Physico‐chemical
properties
protein length:187 AA
molecular weight:21425,3 Da
isoelectric point:8,21
hydropathy:-0,52
Representative Protein Details
Accession
1hMzx
Protein name
1hMzx
Sequence length
292 AA
Molecular weight
32785,90840 Da
Isoelectric point
8,75360
Sequence
MSFKMKYEITKKYLTSGTKRRSGIKMPSVKFVVAHDTGNKGSTALNNVTYYENTKNETSASAHLFVDDKFIYECIPALTGTPEKAWHVLYNKTKDNELYGDDSNDVAIGVEYCYGNNINSAESYKRYVWVIAYICYKFNLDPKKAITAHFILDPQRKSDPKSGLADSGRTYEQLLKDIVSEYEDCTKPEIVSPVVNPPVLPDIPTKIIFTSDNQVVDGFMRNGSNYIPASTLRDKLGDILEWNNNNKILKVNGTQVTAIMKDGMNYVSAQSVISLGHKVTPNGVNKKLYISK
Other Proteins in cluster: phalp2_29706
Total (incl. this protein): 5 Avg length: 287,2 Avg pI: 8,02
Protein ID Length (AA) pI
1hMzx 292 8,75360
2tI5r 247 6,52025
7xkxx 292 8,28079
A0A2Z3EAE0 418 8,31173
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_6407
bpom
 66 62,4% 181 5.112E-82
2 phalp2_321
6SgOZ
 68 46,1% 193 1.017E-53
3 phalp2_18561
73HNe
 82 38,8% 198 8.871E-38
4 phalp2_9318
7vPuw
 15 25,9% 285 8.670E-19
5 phalp2_22283
7wMS5
 3 26,9% 301 1.039E-16
6 phalp2_39055
7ordh
 68 28,6% 185 1.123E-15
7 phalp2_27158
38a6k
 24 26,2% 187 4.952E-15
8 phalp2_424
7xFGZ
 10 31,4% 197 1.278E-13
9 phalp2_13982
kgnW
 11 27,9% 197 3.091E-13
10 phalp2_23523
75EkF
 74 26,9% 252 1.801E-12

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 1hMzx (292 AA)
Member sequence: A0A6J7WLT7 (187 AA)
1 292 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured Caudovirales phage
[NCBI] 		2100421 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LR796223 [NCBI]
CDS location
range 14846 -> 15409
strand +
CDS
ATGGGATTCAAAATGAAGTATGAAATCAAGAAGCAATATCTAACGATTCCGAGTAATCGTAGTAGTGGAAAACCAATGGATAAAATTGTTTTCCTAGTTGCTCATGATACCGGAAATCCAGGATCAACCGCAAAAAATAATGTTGATTACTATGAACGATCACGCGATGAAATTAGTGCATCCGCACATATCTTTGTTGATGATAAAGAAATTATTGAATGTGTTCCATTTCTAACCGATAAACCATTAAAAGCTTGGCACGTCCTTTACAATGTAGACAATGACAATCGTCTTTATAATGCGAATGCAAATGATTCTGCAGGGGGTTTTGAATTGTGTTATGGTGGGAAGATAAACCTAGAGGAATCATACAAGCGTTATGTGTGGGTAATGGCATATGCTTGTCATGTGTACAAGCTAGACCCGATGGCGAAGATTACTGGTCACTACATTCTCGATCCGAAACGCAAGACCGATCCACAATTGAGTTTGAAACTTTTGAACAAAACACTAAACGATTTAATCAAAGATGTATCGAAGGAATTAAAGGATTGCATGAGTTAA
CDS Source ID
[NCBI]
CDS Source
LR798245 [NCBI]
CDS location
range 23795 -> 24358
strand -
CDS
ATGGGATTCAAAATGAAGTATGAAATCAAGAAGCAATATCTAACGATTCCGAGTAATCGTAGTAGTGGAAAACCAATGGATAAAATTGTTTTCCTAGTTGCTCATGATACCGGAAATCCAGGATCAACCGCAAAAAATAATGTTGATTACTATGAACGATCACGCGATGAAATTAGTGCATCCGCACATATCTTTGTTGATGATAAAGAAATTATTGAATGTGTTCCATTTCTAACCGATAAACCATTAAAAGCTTGGCACGTCCTTTACAATGTAGACAATGACAATCGTCTTTATAATGCGAATGCAAATGATTCTGCAGGGGGTTTTGAATTGTGTTATGGTGGGAAGATAAACCTAGAGGAATCATACAAGCGTTATGTGTGGGTAATGGCATATGCTTGTCATGTGTACAAGCTAGACCCGATGGCGAAGATTACTGGTCACTACATTCTCGATCCGAAACGCAAGACCGATCCACAATTGAGTTTGAAACTTTTGAACAAAACACTAAACGATTTAATCAAAGATGTATCGAAGGAATTAAAGGATTGCATGAGTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (1hMzx) rather than this protein.
PDB ID
1hMzx
Method AlphaFoldv2
Resolution 91.57
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50