Protein

Protein accession
A0A6J5RXX7 [UniProt]
Representative
6I81M
Source
UniProt (cluster: phalp2_35242)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTRIPNTDWRPVKNFTKGSVNRPARGVVIHTAEGSFEGTIAWQNNAASSVSSHVVVAKDGRAAQCVDLDDKAWCQAAGNTDWLSIECEGFGSRGETLTSAQVNTIAHIFAWAHTHYGIPLAATDDPINGRGLGWHGMGGSKWGAHYHCPGAQIVAQRVDIINIAAALSGQAPVAPVAPVGPLVDLYQAAKRLAGTIGSGPILRRGDHGFAVRDLQFALIAGAAQQVSVDGQFGGQTETAVKNVQRFFHLPTVDGIVGGQTRRLLAEVLRRKYP
Physico‐chemical
properties
protein length:273 AA
molecular weight:29000,4 Da
isoelectric point:8,98
hydropathy:-0,11
Representative Protein Details
Accession
6I81M
Protein name
6I81M
Sequence length
301 AA
Molecular weight
30781,51900 Da
Isoelectric point
4,92046
Sequence
MPGAQWRGPTPNSTPGGMADHRGLVVHVAEGSYEGTISWCLNPAAAVSYHFIVGKGGQIAQLVDTADKAWAQASGNPYWVSVGCEGYASEGAVTEGQQRALDDVWRWMLDAHGPPNVLTDDPNGAGLGWHGMGGDAWGGHHGCPGEARKAYRSSMVAHAGGAPAAGPDAPTTKEGGAVDICPTPSGNGYYIVDSVGAVFTYGDAVMQGGANDRDLVAPIVGMAVRPQNDGYWLAAADGGVFTFGNAPYKGSRGGEQPKDNAATVAIAAADDGEGYWLLNADGGVFNFGSAPFKGAATGHVH
Other Proteins in cluster: phalp2_35242
Total (incl. this protein): 4 Avg length: 267,3 Avg pI: 5,73
Protein ID Length (AA) pI
6I81M 301 4,92046
4EgIq 258 4,32234
6EGrY 237 4,67946
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_10922
4ERj9
 80 36,1% 257 1.598E-65
2 phalp2_27683
6Iftm
 24 36,0% 250 1.036E-34
3 phalp2_9583
1oWJN
 9 26,1% 275 2.294E-19
4 phalp2_22051
5zyOa
 14 25,8% 317 1.025E-18

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 6I81M (301 AA)
Member sequence: A0A6J5RXX7 (273 AA)
1 301 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured Caudovirales phage
[NCBI] 		2100421 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LR796844 [NCBI]
CDS location
range 8646 -> 9467
strand -
CDS
ATGACTCGAATCCCGAATACTGATTGGCGACCTGTCAAGAACTTCACGAAAGGCAGCGTCAACAGACCTGCTCGCGGCGTAGTCATTCACACCGCTGAAGGCTCATTCGAAGGCACGATCGCATGGCAGAACAATGCTGCGTCAAGCGTGTCATCACATGTCGTCGTCGCGAAAGACGGGCGCGCTGCTCAATGTGTCGACCTCGATGACAAGGCTTGGTGTCAAGCAGCAGGCAACACAGATTGGCTCTCAATCGAGTGCGAAGGGTTCGGCAGTCGAGGCGAGACTCTGACTTCAGCACAGGTCAACACGATCGCTCACATCTTCGCTTGGGCGCATACTCACTACGGCATACCGCTCGCTGCGACTGACGACCCGATCAACGGTCGCGGTCTCGGGTGGCATGGCATGGGCGGCTCAAAGTGGGGCGCGCATTATCATTGCCCGGGCGCTCAGATCGTCGCTCAACGAGTCGACATCATCAACATCGCAGCAGCGCTCTCAGGTCAAGCACCTGTCGCTCCTGTCGCTCCCGTCGGCCCGCTTGTCGATCTCTATCAAGCAGCGAAGCGTCTCGCAGGTACGATCGGCTCAGGCCCGATCTTGCGTCGAGGTGATCACGGCTTCGCTGTACGCGATCTTCAGTTCGCATTGATCGCAGGCGCAGCGCAACAAGTCTCAGTCGACGGTCAGTTCGGCGGTCAAACAGAGACTGCTGTCAAGAATGTTCAACGATTCTTCCATCTGCCTACGGTCGACGGTATTGTCGGCGGGCAGACTCGCAGACTTCTCGCTGAAGTGTTGCGTCGCAAGTATCCCTGA
CDS Source ID
[NCBI]
CDS Source
LR797254 [NCBI]
CDS location
range 39102 -> 39923
strand -
CDS
ATGACTCGAATCCCGAATACTGATTGGCGACCTGTCAAGAACTTCACGAAAGGCAGCGTCAACAGACCTGCTCGCGGCGTAGTCATTCACACCGCTGAAGGCTCATTCGAAGGCACGATCGCATGGCAGAACAATGCTGCGTCAAGCGTGTCATCACATGTCGTCGTCGCGAAAGACGGGCGCGCTGCTCAATGTGTCGACCTCGATGACAAGGCTTGGTGTCAAGCAGCAGGCAACACAGATTGGCTCTCAATCGAGTGCGAAGGGTTCGGCAGTCGAGGCGAGACTCTGACTTCAGCACAGGTCAACACGATCGCTCACATCTTCGCTTGGGCGCATACTCACTACGGCATACCGCTCGCTGCGACTGACGACCCGATCAACGGTCGCGGTCTCGGGTGGCATGGCATGGGCGGCTCAAAGTGGGGCGCGCATTATCATTGCCCGGGCGCTCAGATCGTCGCTCAACGAGTCGACATCATCAACATCGCAGCAGCGCTCTCAGGTCAAGCACCTGTCGCTCCTGTCGCTCCCGTCGGCCCGCTTGTCGATCTCTATCAAGCAGCGAAGCGTCTCGCAGGTACGATCGGCTCAGGCCCGATCTTGCGTCGAGGTGATCACGGCTTCGCTGTACGCGATCTTCAGTTCGCATTGATCGCAGGCGCAGCGCAACAAGTCTCAGTCGACGGTCAGTTCGGCGGTCAAACAGAGACTGCTGTCAAGAATGTTCAACGATTCTTCCATCTGCCTACGGTCGACGGTATTGTCGGCGGGCAGACTCGCAGACTTCTCGCTGAAGTGTTGCGTCGCAAGTATCCCTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6I81M) rather than this protein.
PDB ID
6I81M
Method AlphaFoldv2
Resolution 90.98
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50