Protein

Protein accession
A0A6J5PIY8 [UniProt]
Representative
4Npcy
Source
UniProt (cluster: phalp2_10971)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MGSWQAGHPTGITLHHTADRSLPHVIQYGIAENVGYHLIIDRDGSIHQTANLNYKIYHAGKAMWLKESPNRNHIAVAVISWGRVERIGDEFKSWTGAKIPHDLVAHREGGYWDEATAAQEASLWEVIRWLVKEFKIDPKKICGHSECAQPKGRKDDPKGVLSLTMDEIRQRFSF
Physico‐chemical
properties
protein length:174 AA
molecular weight:19746,2 Da
isoelectric point:7,87
hydropathy:-0,52
Representative Protein Details
Accession
4Npcy
Protein name
4Npcy
Sequence length
199 AA
Molecular weight
21682,67050 Da
Isoelectric point
9,01553
Sequence
MLPDLYPGMKKLVDPLSMSTGRMEASGITVHYLAEVNVEAAMASLRKEGLGYHVIIDRDGKLYQTCYFSHKVAHAGVAKWNGKSPNSNHIAVAVASWGAVGLKNKKFYSWAGHEIPAGEVARRPGNRGADLYHWHAATASQEACLLMFLRWAVSKGINPDEICGHDECAIPEGRKADPGGVLTWTMKGLRDVLKTRPGS
Other Proteins in cluster: phalp2_10971
Total (incl. this protein): 37 Avg length: 197,0 Avg pI: 8,40
Protein ID Length (AA) pI
4Npcy 199 9,01553
1COZj 190 8,30406
1Cr21 216 7,19379
1oWzS 201 6,54395
2cN6s 199 7,71040
3QemJ 195 7,85065
3S89x 207 9,45566
40q2y 199 8,91406
42eLz 185 8,92773
42hjF 185 8,79125
451qR 193 6,29852
45ELh 202 6,36929
469EG 202 7,77327
4BXN2 209 6,70435
4G72G 202 9,24878
4SLGw 203 7,69233
4cdeJ 185 8,97595
4rrle 213 7,09467
4zbEN 206 9,23885
5AFl1 185 9,09715
5lkHy 186 9,27934
6PX2I 205 8,68417
6Q6A6 202 9,23679
6ReR2 186 8,33636
6Rn9E 215 7,15111
6VNz9 202 8,84411
6VQ5d 202 9,19160
6VQVY 210 9,24259
6VRcK 203 9,31815
6VxXP 140 9,42665
8dm2j 204 8,47742
FPTH 194 9,34722
TmYd 187 8,97943
Y4hs 216 9,37959
lWKJ 201 8,32759
xMqX 187 8,47458
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_24979
ze0C
 8 29,2% 178 1.664E-35
2 phalp2_31152
3LHkO
 133 29,4% 173 2.042E-34
3 phalp2_2213
4fsrt
 110 32,3% 176 1.172E-29
4 phalp2_31437
3j8r5
 5 23,7% 177 1.280E-15
5 phalp2_2899
AiYm
 11 20,2% 227 2.370E-15
6 phalp2_18165
4uKtb
 1 26,8% 190 2.771E-14
7 phalp2_4783
4YJpR
 19 22,1% 199 3.767E-14
8 phalp2_11760
45aqa
 242 29,0% 155 1.745E-13
9 phalp2_11916
2xqos
 4 21,1% 184 1.745E-13
10 phalp2_9073
5weQ7
 5 22,6% 199 2.018E-12

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 4Npcy (199 AA)
Member sequence: A0A6J5PIY8 (174 AA)
1 199 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured Caudovirales phage
[NCBI] 		2100421 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LR796852 [NCBI]
CDS location
range 10322 -> 10846
strand -
CDS
GTGGGGTCTTGGCAAGCTGGTCATCCGACTGGTATCACGCTACATCACACAGCTGACCGCTCGTTACCGCACGTCATCCAATATGGTATAGCTGAAAACGTAGGCTATCATTTGATCATTGACCGCGACGGGTCGATACACCAAACCGCAAATTTAAATTATAAAATTTACCACGCCGGTAAAGCTATGTGGCTCAAGGAATCGCCAAACCGCAATCATATTGCCGTCGCGGTTATTTCTTGGGGGCGGGTGGAACGTATCGGCGACGAGTTCAAGTCGTGGACGGGCGCCAAGATACCGCATGACTTGGTGGCGCATCGCGAAGGCGGGTATTGGGACGAGGCTACGGCGGCTCAGGAGGCTTCATTATGGGAAGTTATTCGGTGGCTCGTGAAAGAGTTTAAAATTGATCCTAAGAAGATATGCGGTCACAGCGAGTGCGCTCAACCCAAGGGCCGCAAGGATGATCCCAAGGGCGTTTTGTCGTTGACGATGGATGAGATTAGGCAGAGGTTTTCTTTTTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (4Npcy) rather than this protein.
PDB ID
4Npcy
Method AlphaFoldv2
Resolution 93.12
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50