Protein

Protein accession
A0A6J5N984 [UniProt]
Representative
1Z9i0
Source
UniProt (cluster: phalp2_18899)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 90% (predicted by ML model)
Protein sequence
MAELFGINAKHLIESVPEALDPCEFEIKFDKVKGYKITPRVIVVHYGVTRSQKELEQALLASDYVSAHVALTARGSVNKVTQLVPTNIQAGHAGTNAVWRGQPNVNSFSLGIEINNPGPLFRGADGLFRDVYGRLWDEEEPLAAAHASGRFPAWKLWAPYTAKELQIVELLCRSWIRKYPSIVDVVGHDEIRRDKADPGPAFPMLELRDRLFGS
Physico‐chemical
properties
protein length:214 AA
molecular weight:23861,0 Da
isoelectric point:6,60
hydropathy:-0,20
Representative Protein Details
Accession
1Z9i0
Protein name
1Z9i0
Sequence length
184 AA
Molecular weight
20593,23050 Da
Isoelectric point
7,73553
Sequence
MAVAKVKAPIVWKRALLTNGAYRARPGGPDDIKCIVLHQTCSGSNVAAMPIRDYFNSLRANRVSSHYVLGKESPRCVIVECVPDDMEAWHAGSPSSWHGRSSVNGFSIGIEIVDDGDGRAADSWPDRQIQALAYLLRVLFERHDLDMSDVTEHHLVLRTGERSDLTPDFPWVTLKKYVKNPPWK
Other Proteins in cluster: phalp2_18899
Total (incl. this protein): 2 Avg length: 199,0 Avg pI: 7,17
Protein ID Length (AA) pI
1Z9i0 184 7,73553
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_11760
45aqa
 242 37,5% 152 4.563E-21
2 phalp2_22919
33qi6
 180 32,9% 197 3.307E-16
3 phalp2_11551
100RI
 7 31,3% 166 2.965E-13
4 phalp2_12341
2FfN
 1 33,1% 145 2.965E-13
5 phalp2_31152
3LHkO
 133 30,5% 177 8.734E-12
6 phalp2_15960
4SRAA
 2 28,4% 130 5.498E-11
7 phalp2_31879
4GdWf
 8 33,8% 133 6.346E-10
8 phalp2_24979
ze0C
 8 26,2% 179 8.610E-10
9 phalp2_11251
6GtZw
 14 28,3% 173 1.168E-09
10 phalp2_11676
1LRu7
 24 31,9% 169 1.584E-09

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 1Z9i0 (184 AA)
Member sequence: A0A6J5N984 (214 AA)
1 184 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured Caudovirales phage
[NCBI] 		2100421 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LR796623 [NCBI]
CDS location
range 49994 -> 50638
strand +
CDS
ATGGCCGAGCTGTTTGGAATCAACGCAAAACACTTGATCGAGAGCGTCCCGGAGGCGCTCGATCCGTGCGAGTTCGAGATCAAGTTCGACAAGGTGAAGGGCTACAAAATCACGCCTCGGGTGATCGTGGTCCACTACGGCGTGACGCGCTCTCAGAAGGAGCTGGAGCAGGCCCTGCTGGCCAGTGACTACGTCAGCGCTCACGTGGCGCTCACGGCGCGCGGTAGCGTCAACAAGGTGACGCAATTGGTCCCGACCAACATCCAGGCCGGCCACGCGGGGACCAATGCGGTTTGGCGCGGTCAGCCCAACGTGAACAGCTTCTCGCTCGGGATCGAGATCAACAACCCTGGCCCGCTCTTTCGCGGGGCGGACGGATTGTTCCGCGACGTATACGGCCGGCTCTGGGATGAGGAGGAGCCGCTGGCAGCTGCGCACGCCAGTGGGCGCTTCCCAGCGTGGAAGCTCTGGGCTCCCTACACGGCCAAGGAATTGCAGATCGTGGAGCTGCTGTGTCGGTCGTGGATCCGCAAGTACCCGTCGATCGTCGACGTCGTGGGCCACGACGAGATCCGGCGCGACAAGGCCGACCCTGGCCCGGCGTTCCCCATGTTGGAACTCCGCGATCGGCTGTTCGGCAGCTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (1Z9i0) rather than this protein.
PDB ID
1Z9i0
Method AlphaFoldv2
Resolution 91.10
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50