Protein

Protein accession
A0AAE7KCM8 [UniProt]
Representative
13cSu
Source
UniProt (cluster: phalp2_18764)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVKITKNLSYNNNYYRERNSKLYITIHETANRSVGANANAHANFINNGSQETWHYTVDDTHAVQHFYHTTSCWHAATYKGNTQSIGIEMCVNRDGNYLKTLQNTIELVKKIMKQENIPASRVVQHHFWSGKNCPTLLREGTHGMTWNEFILNVKGQSKRKKPKGWSENPYGTYYKKVDKTFIVGSEKIETRIGSPFLSAPSGGHVQPNQKMTFDYLAQQDGYEWGQLENNRGQQEFVPIRPLSQKEYWGILK
Physico‐chemical
properties
protein length:252 AA
molecular weight:29108,4 Da
isoelectric point:9,39
hydropathy:-0,83
Representative Protein Details
Accession
13cSu
Protein name
13cSu
Sequence length
380 AA
Molecular weight
43264,08460 Da
Isoelectric point
7,62929
Sequence
MVKIVDKFSYNQNYYPETNKRKYITIHETANTGVGADADAHANFINNGAMETWHYTVDSEKAVCHYYHTTSCWACGTYKGNTESISIEMCVNSDGDYLKTLHNTMELVQKIMKEENISANNVVQHHYWSGKNCPTLLREGNSGMNWEQFIAGVKGEKVETKAPSKKDVPKGWDGVNKHGTIYKKEKATFIVGSKEIETRIGAPFRTVKSGGFVQPKQEINYDWVCLQDHHVWVQLENDRGEQEFVPVRTWNEKTGEVGKAWGDFKSGVKGEKVETKAPSKKDVPKGWDGVNKHGTIYKKEKATFIVGSKEIETRIGAPFRTVKSGGFVQPKQEINYDWVCLQDHHVWVQLENDRGEQEFVPVRTWNEKTGEVGKAWGDFK
Other Proteins in cluster: phalp2_18764
Total (incl. this protein): 4 Avg length: 288,8 Avg pI: 8,87
Protein ID Length (AA) pI
13cSu 380 7,62929
1RhyS 271 9,09760
A0AAE7SID6 252 9,37340
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_20292
2aQ1n
 8 62,5% 272 2.116E-123
2 phalp2_33474
7qPh3
 3 27,0% 259 1.189E-29
3 phalp2_7790
7qCWn
 51 32,4% 274 7.885E-28
4 phalp2_24730
6BIOG
 3 25,6% 292 1.557E-08
5 phalp2_867
88GyH
 23 22,7% 255 2.737E-08

Domains

Domains [InterPro]
Ami2
SH3_5
SH3_5
Representative sequence (used for alignment): 13cSu (380 AA)
Member sequence: A0AAE7KCM8 (252 AA)
1 380 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08460

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage BESEP3
[NCBI] 		3071005 Rountreeviridae > Andhravirus > Andhravirus besep3
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MT596500 [NCBI]
CDS location
range 11804 -> 12562
strand -
CDS
ATGGTAAAAATTACTAAAAATTTAAGTTACAACAATAATTATTACAGAGAACGTAATTCAAAACTTTATATTACAATTCATGAAACAGCAAATCGAAGTGTGGGTGCAAATGCAAACGCTCATGCAAATTTCATAAATAATGGTTCACAGGAAACATGGCATTATACCGTTGATGATACACATGCAGTACAACACTTTTATCACACAACTTCATGTTGGCATGCGGCAACATATAAAGGTAATACACAATCCATTGGTATTGAAATGTGTGTCAACCGTGACGGAAATTATTTAAAAACATTACAAAATACAATTGAATTAGTTAAAAAAATTATGAAACAAGAAAATATTCCTGCTAGTCGTGTTGTTCAACATCATTTTTGGAGTGGTAAAAACTGTCCTACTCTATTACGTGAAGGGACACATGGTATGACATGGAATGAGTTTATTTTAAATGTTAAAGGTCAATCAAAACGTAAAAAACCTAAAGGTTGGAGTGAAAATCCTTATGGTACTTACTATAAAAAAGTTGATAAAACATTTATCGTAGGTTCAGAAAAAATTGAAACACGTATTGGTTCACCTTTCTTATCTGCACCAAGTGGAGGACACGTACAACCAAATCAAAAAATGACCTTTGACTATTTAGCACAACAAGATGGTTATGAATGGGGACAACTTGAAAATAATAGAGGACAACAAGAATTTGTACCAATTAGACCATTAAGTCAAAAAGAATATTGGGGTATTTTAAAATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (13cSu) rather than this protein.
PDB ID
13cSu
Method AlphaFoldv2
Resolution 88.13
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50