Protein

Protein accession
A0A6J5M318 [UniProt]
Representative
49SM8
Source
UniProt (cluster: phalp2_39279)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MMRHLFSRMLEVGRNIDIRPKPKPAKPDPMAEVDKALNISVKPEVVLPPVTHPARKTSKAGIDLLHQFEGCAKVRPDGKVEAYLCPAGVWTIGWGSTGNDPFNGGKIGKGTVWTKEQCDTRFEQHLVQFERAVLDGLGNNPASQAQFDAMVCLTYNIGPAAFARSTVLRKHNGGDFPAAEKAFLMWNKAGGKVLRGLIRRREAEAALYRSGS
Physico‐chemical
properties
protein length:212 AA
molecular weight:23250,6 Da
isoelectric point:9,63
hydropathy:-0,37
Representative Protein Details
Accession
49SM8
Protein name
49SM8
Sequence length
261 AA
Molecular weight
28425,51350 Da
Isoelectric point
9,14640
Sequence
MGIAQVISHGLTTATGMNVQGVILIALALVSEMEQGTYRQVAFIVVLLIWAFISFITRGHEALPARIDEPMDDLDHDAPIDDILAQGRSLLDDPNFTKAISGQYNTPLGLMATPALPTMAVSQRGIDLIKQFEGCKLTAYWDKWGKVWTIGYGWTIGVKKGDKWTQAKAEKMLAEGVKPYAKAVADAIGTAPTTQSAFDAMSSFCYNAGPANFRKSSMLRFHKQGNRTAAGNAFLAWNKAGGVVLKGLVRRREAERALYLS
Other Proteins in cluster: phalp2_39279
Total (incl. this protein): 4 Avg length: 244,3 Avg pI: 9,38
Protein ID Length (AA) pI
49SM8 261 9,14640
1kTP3 251 9,62295
3ZGr 253 9,12855
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_16734
W5W8
 3 38,1% 165 2.246E-26
2 phalp2_35853
4JYw2
 2 30,1% 176 3.997E-20
3 phalp2_29359
13nsB
 1 31,9% 166 5.780E-17

Domains

Domains [InterPro]
Disordered region
GH24
Representative sequence (used for alignment): 49SM8 (261 AA)
Member sequence: A0A6J5M318 (212 AA)
1 261 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF00959

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured Caudovirales phage
[NCBI] 		2100421 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LR796360 [NCBI]
CDS location
range 37287 -> 37925
strand +
CDS
ATGATGCGCCATCTCTTTAGTCGGATGTTAGAGGTGGGGCGCAATATCGACATTCGCCCCAAGCCCAAGCCCGCCAAGCCTGATCCTATGGCCGAGGTGGACAAGGCTCTCAACATTTCTGTCAAGCCGGAGGTCGTCTTGCCGCCCGTGACCCACCCCGCCCGCAAGACGAGTAAAGCAGGTATCGACCTGCTGCACCAGTTCGAGGGCTGCGCCAAGGTGCGCCCTGACGGCAAGGTCGAAGCCTATCTTTGCCCGGCAGGTGTCTGGACGATTGGTTGGGGCAGCACCGGCAACGACCCGTTCAACGGCGGCAAGATCGGTAAAGGAACGGTCTGGACGAAAGAGCAGTGCGACACCCGCTTCGAGCAGCATCTTGTGCAGTTCGAGAGGGCTGTGCTTGATGGTCTCGGCAATAACCCCGCCAGCCAAGCACAGTTTGATGCGATGGTCTGTCTGACCTACAACATCGGCCCCGCGGCCTTCGCTCGTTCGACTGTCTTGCGCAAGCACAACGGTGGCGACTTCCCTGCTGCGGAAAAGGCGTTCTTGATGTGGAACAAGGCTGGCGGCAAAGTGTTGCGCGGTCTTATCCGCCGCCGCGAGGCGGAAGCTGCATTGTATAGGAGCGGATCATGA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016998 cell wall macromolecule catabolic process biological process None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 None Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00000632

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (49SM8) rather than this protein.
PDB ID
49SM8
Method AlphaFoldv2
Resolution 77.64
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50