Protein

Protein accession
Q9AZ81 [UniProt]
Representative
n1oN
Source
UniProt (cluster: phalp2_36349)
Protein name
lysozyme
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MLKMVDVYSGSPRSFATQSGTDITMVKATQGTYYVNPYCDTDYQAAKKAGKMLGVYHYAGGGDPAVEANYFYKNTKNYIGEAVPALDWEDYQNPKFGKDSNWCREFVDKYHELSGVWPLIYTGQASLSEVGNCAKDCGLWVAWYATMNWNSWTLPTASFSVAPWPTYTIWQFTGGDMDRNVVNTTREGWQKLAKPNVDGRPVSQPVIAETKLRPEVKKWTDDLGDVWYSEKGTFVTSGAINLRYGARTSSKIIAQLPAGTEVKYDAYSRHDGYVWIRQPRSNGYGYLVCRAGNEAWGTFK
Physico‐chemical
properties
protein length:300 AA
molecular weight:33764,4 Da
isoelectric point:8,11
hydropathy:-0,53
Representative Protein Details
Accession
n1oN
Protein name
n1oN
Sequence length
152 AA
Molecular weight
17033,06990 Da
Isoelectric point
5,27019
Sequence
MLKMVDVFSGSPRSFATLPETDITMVKATQGTGYVNPACNIDYANAKAAGKLLGLYHYCAGGNPIAEADYFINNIKNYVGEAVLAVDWEGYQNASWGNYGYVRQFVNRVHELTGVWCMVYVSQSEIRQVANCVNDCPLWVAYYKYSQPLNWD
Other Proteins in cluster: phalp2_36349
Total (incl. this protein): 6 Avg length: 204,7 Avg pI: 6,79
Protein ID Length (AA) pI
n1oN 152 5,27019
5LzId 181 6,03121
64FW0 156 5,42428
79GRi 193 9,20578
Q20DB2 246 6,72487
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_38994
84ePK
 348 50,7% 132 1.233E-45
2 phalp2_20117
23DDy
 70 43,3% 127 3.931E-34
3 phalp2_39219
3xEyz
 21 36,6% 150 6.211E-29
4 phalp2_2231
4klcC
 109 36,8% 133 6.374E-23
5 phalp2_3635
4SEzC
 3 28,6% 122 6.380E-20
6 phalp2_20806
5TBA5
 9 34,7% 115 1.613E-16
7 phalp2_5615
4g2xP
 40 29,1% 134 2.206E-16
8 phalp2_34559
4UNXW
 287 34,5% 136 3.681E-15
9 phalp2_36157
6TiaL
 25 28,9% 128 1.756E-14
10 phalp2_19241
7XkuR
 1 30,0% 140 1.756E-14

Domains

Domains [InterPro]
GH25
Representative sequence (used for alignment): n1oN (152 AA)
Member sequence: Q9AZ81 (300 AA)
1 152 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01183

Taxonomy

  Name Taxonomy ID Lineage
Phage Lactobacillus prophage Lj771
[NCBI] 		139871 No lineage information
Host Lactobacillus johnsonii
[NCBI] 		33959 Firmicutes > Bacilli > Lactobacillales > Lactobacillaceae > Lactobacillus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AF195902 [NCBI]
CDS location
range 39195 -> 40097
strand +
CDS
ATGCTTAAAATGGTTGATGTATATAGTGGAAGCCCTAGAAGTTTTGCAACTCAAAGTGGTACTGATATCACTATGGTAAAAGCTACTCAAGGAACTTACTATGTAAACCCATACTGTGATACCGATTATCAGGCTGCTAAGAAAGCTGGAAAAATGCTTGGGGTATATCACTATGCTGGTGGTGGCGATCCTGCTGTTGAAGCTAATTATTTCTATAAAAATACCAAGAATTACATTGGCGAAGCAGTTCCCGCCTTAGATTGGGAAGATTATCAAAATCCTAAATTCGGTAAAGACTCAAATTGGTGTAGAGAGTTTGTTGATAAGTACCACGAATTATCTGGTGTTTGGCCTCTTATATATACTGGTCAAGCTTCACTTTCTGAAGTTGGAAATTGTGCGAAAGATTGTGGTCTATGGGTAGCTTGGTACGCTACTATGAATTGGAACAGTTGGACTTTACCGACTGCAAGCTTTAGTGTTGCGCCTTGGCCAACTTATACCATCTGGCAGTTTACGGGTGGTGATATGGATCGCAACGTGGTTAACACGACCCGTGAAGGTTGGCAAAAGTTAGCTAAGCCTAATGTTGATGGAAGACCTGTATCACAACCAGTAATTGCAGAAACAAAACTACGTCCAGAAGTTAAGAAATGGACTGATGATCTCGGGGATGTTTGGTACTCTGAAAAGGGCACTTTCGTAACGAGCGGAGCAATCAATCTTAGATATGGAGCTAGAACTTCATCTAAAATTATTGCTCAACTCCCAGCAGGTACAGAAGTTAAGTATGATGCTTATAGCCGACACGATGGATATGTCTGGATTAGACAACCTCGATCAAATGGATACGGATACTTAGTATGTCGTGCTGGTAATGAGGCATGGGGAACTTTTAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016052 carbohydrate catabolic process biological process None (UniProt)
GO:0016998 cell wall macromolecule catabolic process biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 None Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00000632

Tertiary structure

PDB ID
upi0001627b00_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (n1oN) rather than this protein.
PDB ID
n1oN
Method AlphaFoldv2
Resolution 95.52
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50