Protein

Protein accession
Q5NTY4 [UniProt]
Representative
8lSNT
Source
UniProt (cluster: phalp2_31265)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MKTKAQAKSWINSKIGKGIDWDGMYGYQCMDEAVDYIHHVTDGKVTMWGNAIDAPKNNFQGLCTVYTNTPEFRPAYGDVIVWSYGTFATYGHIAIVVNPDPYGDLQYITVLEQNWNGNGIYKTEFATIRTHDYTGVSHFIRPKFADEVKETAKTVNKLSVQKKIVTPKNSVERIKNYVKTSGYINGEHYELYNRGHKPKGVVIHNTAGTASATQEGQRLTNMTFQQLANGVAHVYIDKNTIYETLPEDRIAWHVAQQYGNTEFYGIEVCGSRNTDKEQFLANEQVAFQEAARRLKSWGMRANRNTVRLHHTFSSTECPDMSMLLHTGYSMKNGKPTQDITNKCADYFMKQINAYIDGKQPTSTVVGSSSSNKLKAKNKDKSTGWNTNEYGTLWKKEHATFTCGVRQGIVTRTTGPFTSCPQAGVLYYGQSVNYDTVCKQDGYVWISWTTSDGYDVWMPIRTWDRSTDKVSEIWGTIS
Physico‐chemical
properties
protein length:477 AA
molecular weight:53982,9 Da
isoelectric point:8,73
hydropathy:-0,61
Representative Protein Details
Accession
8lSNT
Protein name
8lSNT
Sequence length
352 AA
Molecular weight
38970,60580 Da
Isoelectric point
9,59601
Sequence
MVEIINKTVTRGVAGRRPGAVKGVVFHNTWGNSTAKQEANRLAAMNNNQLAAGFAHYYIDKNTIWRTEDTYNAAWHTANSDGNTNYIGYEVCGNDQTPLKDFLQAEENTFWQIAQDLKYYGLPVNRNTVRLHHEFSATQCPKRSLIIHTGFNSTQAQPANVTNAMKDYVIKNVLKYYNNPSLKPDGKAQSTGGQTPPGGANVTPSTPSQHDKAVAASPAKHQGNAWGKLDYFNGHGKDHIRVAGWLVPDKPQGPIGTYAYVIFMQHGTNKELTRIQSAGIKRPDVKKAYGYQGGQELGFDVTVNKNQFKGKKVDVILRRANKSNGKVIKFTPRVALMFCWTCSNCFIMLAGC
Other Proteins in cluster: phalp2_31265
Total (incl. this protein): 54 Avg length: 326,9 Avg pI: 9,19
Protein ID Length (AA) pI
8lSNT 352 9,59601
17jj9 263 9,82235
1FCyx 325 9,41440
1cgso 326 9,41491
3bUW1 332 9,15549
3mwmh 341 9,63739
4F0Gt 279 9,38822
4lNcP 327 9,34793
5MjyF 341 9,38113
5MnBE 325 9,26219
5NejM 341 9,60490
5Ro1x 302 9,38719
5T4gr 324 6,55418
5VDka 341 9,54379
5Z278 341 9,39422
61HrZ 322 9,46423
69pys 341 9,59169
6bdu2 333 8,60165
6c9Rk 341 9,60490
6gGEJ 341 9,59169
6migj 325 9,19172
6nz1E 304 9,66950
6oHrL 341 9,63759
6pyIn 324 6,31432
6s3KZ 341 9,59149
76j8Y 324 6,55407
7A1Dy 304 9,67189
7AgUx 304 9,60091
7PD0H 341 9,63759
7Xunx 299 9,32182
7Z6pq 341 9,56983
7bPLa 332 9,10772
7jA6F 341 9,56983
7muf5 324 6,31523
7rzFS 336 8,74303
84Wbj 324 6,55418
85Y8s 302 9,53064
8omFK 342 9,33407
8ondz 280 9,62856
8ooyJ 279 9,40047
8tVsE 279 9,62811
D9J0J9 280 9,74654
A0A0M4S679 341 9,63759
A0A060AHF9 341 9,56983
A0A5S9MQ13 342 9,60742
A0A7L8ZJE2 341 9,60503
A0A976MEU2 280 9,74654
A0AAE9V749 341 9,54379
A0AAE9V7F7 341 9,54379
A0AAE9V9I3 341 9,54379
A0AAX4G3U3 287 9,42961
A0AAX4LXP1 374 9,76588
A0AAX4PNF7 341 9,51871
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_20237
8tSRS
 1 95,3% 217 6.604E-118
2 phalp2_10524
86QiV
 42 34,0% 326 1.655E-53
3 phalp2_10050
7qQCo
 2 24,0% 279 2.908E-19
4 phalp2_1214
8MrbY
 36 24,0% 329 1.435E-13
5 phalp2_16558
7rWDg
 20 26,9% 234 1.914E-13
6 phalp2_6225
6oM64
 106 24,5% 216 8.016E-12
7 phalp2_26995
4LbNv
 19 22,2% 305 4.524E-04

Domains

Domains [InterPro]
Ami2
Unannotated
Disordered region
Representative sequence (used for alignment): 8lSNT (352 AA)
Member sequence: Q5NTY4 (477 AA)
1 352 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage phiWMY
[NCBI] 		284052 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AB182649 [NCBI]
CDS location
range 616 -> 2049
strand +
CDS
ATGAAAACTAAAGCACAAGCTAAGTCTTGGATTAACAGTAAAATAGGTAAAGGCATTGATTGGGACGGCATGTACGGTTACCAGTGTATGGACGAGGCGGTAGACTACATTCACCATGTTACAGATGGAAAGGTAACGATGTGGGGTAACGCTATTGATGCACCTAAAAATAATTTTCAAGGTTTATGTACGGTTTATACAAATACACCAGAATTCCGTCCTGCATATGGCGATGTGATTGTATGGTCTTATGGAACATTCGCCACATATGGACACATAGCAATCGTGGTTAATCCCGACCCTTACGGCGACTTACAATACATTACTGTGTTAGAGCAAAATTGGAATGGAAATGGAATTTATAAAACAGAATTCGCAACTATTCGTACACATGACTACACAGGCGTTTCTCATTTTATCCGTCCTAAATTTGCCGACGAAGTAAAAGAAACTGCAAAGACAGTTAACAAGTTATCAGTACAAAAGAAAATAGTTACACCTAAAAATTCTGTAGAACGTATTAAAAATTACGTTAAAACATCTGGTTATATTAATGGAGAACATTACGAATTATATAATCGTGGTCATAAACCTAAAGGTGTAGTTATTCATAACACTGCTGGTACTGCTTCTGCGACACAAGAGGGTCAAAGATTAACAAATATGACTTTCCAACAATTAGCAAATGGAGTTGCACACGTATATATTGATAAAAATACAATTTATGAAACATTACCAGAAGATCGTATCGCATGGCATGTAGCTCAACAGTACGGTAATACAGAATTCTATGGTATTGAGGTGTGTGGCTCACGAAACACTGATAAAGAGCAATTCCTGGCAAATGAACAAGTAGCATTTCAAGAGGCTGCGAGACGTTTAAAAAGTTGGGGAATGCGTGCAAACAGAAACACAGTGAGACTACATCATACATTCTCTAGTACAGAATGCCCGGACATGTCTATGTTACTACACACAGGCTATAGCATGAAGAATGGTAAGCCTACACAAGATATCACAAACAAGTGTGCAGATTACTTTATGAAACAAATTAACGCTTATATAGACGGTAAACAACCAACTAGCACCGTTGTTGGTTCATCATCTAGCAATAAGTTAAAAGCGAAGAACAAAGACAAATCTACAGGTTGGAATACTAATGAATACGGTACCTTATGGAAAAAAGAACACGCTACATTCACATGTGGCGTTAGACAAGGTATCGTGACTAGAACGACAGGACCATTTACATCATGCCCACAAGCAGGCGTGTTATATTATGGTCAATCAGTTAACTATGATACTGTATGTAAACAAGATGGCTACGTTTGGATTAGTTGGACAACAAGTGATGGCTACGATGTATGGATGCCAATTCGCACTTGGGACAGAAGTACAGATAAAGTAAGTGAAATTTGGGGAACAATCAGTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
Q5NTY4
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi000049e434_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (8lSNT) rather than this protein.
PDB ID
8lSNT
Method AlphaFoldv2
Resolution 81.72
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50