Protein

Protein accession
D3WK93 [UniProt]
Representative
7jike
Source
UniProt (cluster: phalp2_22253)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKKTLKHISSVVFAAILALSITTSAVADRVLIIPDLPKQGYRNGVGAYEGVVAHSTATPEAPAINIQRYETRTWRSAFVHYAVDWDEVIQIADTRYIAYGAGPAANARFVHVELCETSDYSKFKRSYDKYVKLLAKILRDRGLSVEKGLWTHDDVRKYLGGTTHTDPLDYLKKHGISEAQFRADVKRAYNNTGISIPEQPSKPAEKPTANVEGVAYIEGYNVNLRKGPDASYSVIRQLNKPEAYKVWGEKGGWLNLGWNQWVKYNPSYIRFEKKESVSPVAGKRVVSKVSNLRFYSAPSWEDKYVAGTVDVGLGFTIDATVMVNGSPQYKVHNSKGTTYYITASEAYVYVK
Physico‐chemical
properties
protein length:351 AA
molecular weight:39281,1 Da
isoelectric point:9,37
hydropathy:-0,39
Representative Protein Details
Accession
7jike
Protein name
7jike
Sequence length
172 AA
Molecular weight
19390,83650 Da
Isoelectric point
7,83737
Sequence
MKKTIKHITSFLTILVLAGSFATSAFADRTLIIPDLPKQPYRYGVGAYEGVVAHSTATPEAPAINIQKYESRTWRNAFVHYAVDWDETIQIADTKYIAYGGGPAANKRFVHVELCETAEYAKFKRSYEKYVKLLAKILKDNKISVEKGLWTHSDVTHHLGGTDHEDPIDYLT
Other Proteins in cluster: phalp2_22253
Total (incl. this protein): 38 Avg length: 262,6 Avg pI: 8,02
Protein ID Length (AA) pI
7jike 172 7,83737
14kSh 224 8,29420
2Gymc 217 6,52167
2k1dT 117 8,73548
2sDRu 208 6,10749
2sDS6 208 5,89371
2td9J 209 5,63640
39BqK 236 8,42133
39yea 230 8,44312
4H4RI 222 5,47754
4MNLp 233 5,77413
4MUg2 238 5,87484
4MZCY 238 6,08094
4SCmT 228 9,25484
4SEFK 220 6,11749
4cdnL 167 6,03314
4yyIY 225 5,38000
71Mk9 230 7,86065
7rGmz 151 8,98478
7rHbt 188 8,49108
7rmRa 212 7,82661
b81C 124 8,99045
fYZu 221 8,44234
A0A1B1P7Q1 350 9,20049
A0A1C8E990 350 9,13370
A0A288WFT1 351 9,41949
A0A288WFW6 351 9,24311
A0A1B0T6C8 350 8,35151
A0A288WFQ3 351 9,32743
A0A7S6NIT4 351 9,31795
A0A7S6NIX4 351 9,19882
A0A7S6NJ04 351 9,19895
A9QTG3 351 9,37050
C7DT94 351 9,40976
A0A1B2APY1 349 9,26844
A0AAE8YWY6 351 9,25200
A0AAE9CCR8 351 9,25200
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_31879
4GdWf
 8 26,7% 131 7.113E-23
2 phalp2_25288
86peP
 187 25,8% 124 2.487E-22
3 phalp2_5052
1q1Q3
 64 29,3% 126 2.707E-20
4 phalp2_5528
3sIWi
 1 31,2% 141 9.444E-20
5 phalp2_11786
8bZ18
 2 25,9% 127 2.409E-19
6 phalp2_18351
5t09q
 43 26,6% 154 6.145E-19
7 phalp2_33007
4igcZ
 220 27,9% 143 1.567E-18
8 phalp2_10025
78NlZ
 4 37,3% 115 3.993E-18
9 phalp2_31178
7Vvn5
 47 30,2% 162 1.897E-17
10 phalp2_16822
1p2ki
 32 21,5% 139 8.999E-17

Domains

Domains [InterPro]
Disordered region
Ami2
Representative sequence (used for alignment): 7jike (172 AA)
Member sequence: D3WK93 (351 AA)
1 172 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage Wip4
[NCBI] 		663236 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
GU142943 [NCBI]
CDS location
range 1 -> 1056
strand +
CDS
ATGAAAAAAACGTTAAAACACATTTCTTCTGTAGTCTTTGCAGCTATTTTAGCGTTATCCATTACAACAAGTGCTGTTGCTGACAGAGTATTGATCATTCCTGATTTGCCGAAGCAAGGATATAGAAATGGGGTAGGAGCTTATGAAGGTGTAGTGGCGCATAGTACAGCGACTCCAGAAGCTCCAGCAATCAATATCCAACGTTATGAAACGCGTACTTGGAGAAGTGCATTTGTACATTATGCAGTTGACTGGGATGAAGTTATTCAAATTGCAGATACACGTTATATTGCGTATGGCGCTGGACCAGCTGCTAACGCACGTTTTGTACACGTTGAACTTTGTGAGACTTCAGATTATAGCAAGTTTAAGCGAAGCTATGACAAGTATGTAAAATTACTAGCAAAAATTTTACGTGATCGTGGACTTTCAGTAGAAAAAGGATTATGGACACATGATGACGTAAGGAAATATCTTGGTGGAACAACTCACACGGATCCACTGGATTATCTAAAAAAACATGGTATATCTGAAGCTCAATTCCGAGCAGATGTAAAACGTGCTTATAATAACACGGGTATTTCTATTCCTGAACAACCTTCTAAACCAGCGGAAAAACCAACAGCCAATGTAGAAGGTGTAGCTTATATTGAAGGATATAATGTAAATCTACGCAAAGGCCCAGATGCAAGCTATTCTGTTATTCGTCAATTAAATAAACCAGAGGCTTATAAGGTTTGGGGAGAAAAGGGCGGATGGCTAAATTTAGGTTGGAATCAGTGGGTAAAATATAATCCATCATATATCCGATTTGAAAAGAAAGAATCTGTAAGTCCAGTTGCTGGAAAACGTGTAGTGTCGAAAGTGAGTAATCTCAGATTTTATAGCGCTCCATCGTGGGAAGATAAGTATGTTGCAGGTACTGTAGATGTAGGATTAGGATTTACGATTGACGCAACGGTAATGGTAAATGGTTCCCCACAATACAAAGTGCACAATAGTAAAGGAACAACATACTATATCACAGCAAGTGAAGCCTATGTGTATGTGAAGTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
upi0001cdcafa_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7jike) rather than this protein.
PDB ID
7jike
Method AlphaFoldv2
Resolution 90.57
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50