Protein

Protein accession
A4JX20 [UniProt]
Representative
8jI3j
Source
UniProt (cluster: phalp2_12784)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGVVGAAAAALLFSVVPKFEGVKLVGYLDPVGIPTKCMGDTRDVVVGRAYSEAECRSSLETQLIAHAEPVLRCTPGLRGRPYQLAAAVSFAYNVGAHAYCNSTTAKRFNAGDLRGACRAINESDSGRRQWVFANCRTVIDPKTKKPLPVCDTLPGLVKRRAEERAICERGL
Physico‐chemical
properties
protein length:171 AA
molecular weight:18397,1 Da
isoelectric point:9,24
hydropathy:-0,07
Representative Protein Details
Accession
8jI3j
Protein name
8jI3j
Sequence length
200 AA
Molecular weight
22238,24170 Da
Isoelectric point
9,24317
Sequence
MRLKFDWGRNSKLGVDSSSNNVKGVKFKALVGAATAAILIGTFGDNATTGFTERHEGMVLRGYKDPIGIPTKCAGDTYNVVLDKVYTYGECIDSLDTQLYNHALPVLKCLPSIRDNVHFLAAAIDHNYHLGNFCSSSMMKAHRAGDDKTACVRYNENSLGKPQYIYVKDKFIDGKWTYKSLNGLIKRAAERRSMCERGLK
Other Proteins in cluster: phalp2_12784
Total (incl. this protein): 17 Avg length: 175,6 Avg pI: 9,21
Protein ID Length (AA) pI
8jI3j 200 9,24317
1lKRD 189 9,29945
34Bm 194 9,08464
4fIRR 164 9,11269
4fwOn 184 9,00038
4gGOz 174 9,31783
7QagF 186 9,01218
8cGuj 195 9,01018
Q6JIK8 163 9,41008
A0A0S0N859 186 9,01218
A0A514CTT7 156 9,22989
A0A514CW31 156 9,22989
A0A5B9N6W7 186 9,13609
Q8W6S6 134 9,68123
A0A9X9NYL1 186 9,01231
A0AA96KH54 162 9,46526
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_11002
4Mz75
 307 33,5% 143 2.649E-39
2 phalp2_35864
4Lj4w
 95 31,4% 181 2.363E-35
3 phalp2_4305
8aoFg
 2477 28,7% 153 9.948E-31
4 phalp2_7028
8i7gz
 89 26,2% 145 7.617E-26
5 phalp2_9410
8Hj1q
 5109 31,0% 145 3.621E-25
6 phalp2_26525
83EoO
 66 26,7% 172 2.637E-20
7 phalp2_21319
1KsyR
 184 30,2% 149 1.694E-19
8 phalp2_30973
1fTML
 10 25,0% 168 5.099E-18
9 phalp2_3556
4E0oc
 53 28,8% 180 4.503E-15
10 phalp2_34995
4LMnK
 728 23,6% 186 4.503E-15

Domains

Domains [InterPro]
Disordered region
GH24
Representative sequence (used for alignment): 8jI3j (200 AA)
Member sequence: A4JX20 (171 AA)
1 200 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF00959

Taxonomy

  Name Taxonomy ID Lineage
Phage Stanholtvirus sv6442
[NCBI] 		431891 Stanholtvirus >
Host Burkholderia pseudomallei
[NCBI] 		28450 Proteobacteria > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Burkholderia > pseudomallei group

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
CP000625 [NCBI]
CDS location
range 21735 -> 22250
strand +
CDS
GTGGGTGTTGTGGGGGCTGCTGCGGCAGCCCTTCTTTTTTCCGTCGTCCCGAAGTTCGAGGGCGTCAAACTGGTCGGGTATCTCGATCCGGTCGGCATCCCGACAAAGTGCATGGGCGACACGCGCGATGTCGTCGTCGGCCGGGCGTACAGCGAGGCCGAGTGTCGCTCGTCACTCGAAACGCAACTGATCGCCCACGCCGAACCCGTGCTGCGTTGCACGCCGGGGCTGAGAGGTCGTCCGTATCAGCTCGCGGCGGCCGTGAGCTTTGCATACAACGTCGGCGCGCATGCCTACTGCAACAGCACGACGGCGAAGCGCTTCAACGCGGGCGACCTGCGCGGCGCGTGCCGCGCGATCAACGAATCCGATAGCGGTCGGCGGCAGTGGGTCTTTGCGAACTGCCGGACCGTTATCGACCCGAAAACGAAAAAGCCTCTGCCGGTATGCGACACGCTACCGGGTCTGGTGAAGCGGCGTGCGGAAGAGCGCGCGATCTGCGAGCGGGGGCTCTGA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016998 cell wall macromolecule catabolic process biological process None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 None Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00000632

Tertiary structure

PDB ID
upi0000f597cf_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (8jI3j) rather than this protein.
PDB ID
8jI3j
Method AlphaFoldv2
Resolution 85.60
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50