Protein

Protein accession
A0A7S5SKF0 [UniProt]
Representative
aMK
Source
UniProt (cluster: phalp2_7826)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVQIRQELVPSYLADDVVYGFDNPCDYIIIHETANTTAGADADAHANLQAKGNSRQASWQYQVDDKEIVQSFKDEAECWHAGSKFYNQNGIGIEICVNSDGDFKKAVANAAYLVRILMKRHGIPKSRVLQHRTTSGWKDCPRFLRSGSKGVDWDGFLAMLDGSAEAPVEAPKPVAPKPKPVAGKPDFDTNSVVDFLVSVGEDSSFSNRSKLAVKHGISGYDGSAAQNTKLLDKLKAAYKAASKPAPAPAPSGKGDMDTNSIVTYLNSIGVDSSYGNRAKLAAQYGIKGYEGSAAQNLALLEKMRNGAKPVAAKPAPKGDMNTGSIVTYLNSIGVDASFSNREKLAAKYGIRGYQGTAAQNTALLEKMRGGAAPVAAKPAPRGDQKTTSLVDYLKSIGEGSSFANREKLARQYGISGYKGTASQNTALLKKMRGH
Physico‐chemical
properties
protein length:434 AA
molecular weight:46378,7 Da
isoelectric point:9,30
hydropathy:-0,46
Representative Protein Details
Accession
aMK
Protein name
aMK
Sequence length
246 AA
Molecular weight
27032,20640 Da
Isoelectric point
9,46236
Sequence
MVTVRKKLVSDAQAAKVTNGKGNTKRKVVIHGTDNYRTGADADAHARLQANGNSRSASWHWQVDDKEAVQSFEHTWKCWAAGTAKGNTEGIHVEMCVNSDGNYRKTVENTAELVAKILKDEGLAITDVVQHNYYSGKNCPREIRAGNVTWAVFLQMVKNAGAIKQPKPITDNNKYRILTGTYSTLQAAENALDVLKHRFGWVAYIEQDDSKWRIKSGTFTGMDAAQAGANKIKTAKLAQVANIIAA
Other Proteins in cluster: phalp2_7826
Total (incl. this protein): 29 Avg length: 299,1 Avg pI: 9,20
Protein ID Length (AA) pI
aMK 246 9,46236
1QZDR 254 8,68062
1d9HY 246 9,35818
1mIvl 252 9,57982
1zKoC 261 7,13093
2mwo 206 6,24805
2mzS 201 6,05019
3xQwH 249 9,51478
44Sa4 281 9,38797
743Oh 313 9,15324
7EVE5 354 9,57557
7knOs 252 9,56977
7plvv 246 9,47919
7qxGD 319 10,00209
CGus 302 9,77865
D50z 299 9,82848
QPin 205 9,48589
fuet 249 9,31692
A0A345MJQ8 367 9,69200
A0A0A0RNZ5 316 9,54424
U5PU59 368 9,66164
A0A2I6UHW0 368 9,67163
A0A384X9J7 368 9,63959
A0A6C0RTQ9 318 9,22261
S6B611 368 9,66164
A0A898KCQ3 295 9,44908
A0AAE9H3I1 368 9,62985
A0AAE9KLF5 368 9,66164
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_6450
8Jtqs
 137 49,2% 189 9.664E-70
2 phalp2_8874
2Yvfy
 25 49,6% 153 3.807E-64
3 phalp2_20292
2aQ1n
 8 44,9% 187 1.205E-62
4 phalp2_29544
3KzTO
 225 43,0% 172 1.650E-59
5 phalp2_33479
7swgL
 97 44,1% 163 3.787E-55
6 phalp2_38352
A4V0
 20 41,6% 192 5.182E-55
7 phalp2_1131
3cMm
 37 34,8% 244 1.444E-49
8 phalp2_5899
5KvkK
 133 32,8% 195 2.489E-40
9 phalp2_259
6ql8e
 14 35,8% 173 6.064E-37
10 phalp2_38485
1mrCF
 6 34,1% 167 8.850E-35

Domains

Domains [InterPro]
Ami2
SPOR
Representative sequence (used for alignment): aMK (246 AA)
Member sequence: A0A7S5SKF0 (434 AA)
1 246 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF05036

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage P59
[NCBI] 		2723893 Herelleviridae >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MT151604 [NCBI]
CDS location
range 21724 -> 23028
strand -
CDS
ATGGTACAAATTAGACAAGAGCTTGTCCCTTCTTATCTTGCTGATGATGTCGTTTACGGTTTCGATAACCCTTGTGATTATATTATTATCCATGAGACAGCCAATACTACAGCAGGTGCGGACGCTGACGCTCACGCTAACCTACAGGCCAAAGGTAACTCTCGTCAGGCTTCATGGCAATACCAAGTTGATGATAAAGAGATCGTACAGTCCTTCAAAGACGAAGCTGAATGTTGGCACGCAGGAAGCAAATTCTACAACCAGAACGGTATCGGAATTGAAATCTGCGTAAACTCTGATGGAGACTTTAAAAAGGCTGTAGCCAATGCTGCATACCTTGTTCGTATCCTTATGAAGCGTCATGGTATCCCTAAGTCCCGTGTATTACAGCATAGAACGACTAGCGGTTGGAAAGACTGCCCAAGATTCTTGCGTAGCGGCTCTAAAGGTGTAGACTGGGATGGTTTCTTAGCAATGTTAGACGGTTCAGCTGAGGCTCCTGTAGAAGCACCTAAGCCAGTAGCACCTAAGCCTAAACCAGTTGCAGGTAAACCAGACTTCGATACTAACAGCGTTGTAGACTTCCTTGTTTCTGTAGGAGAAGATTCTAGCTTTAGTAACCGATCTAAATTAGCGGTTAAGCACGGTATCTCAGGATATGATGGTTCCGCAGCTCAGAACACTAAATTGCTTGACAAACTTAAGGCTGCTTACAAAGCTGCTTCTAAACCTGCCCCTGCTCCGGCTCCATCTGGAAAAGGCGACATGGACACAAACAGCATTGTAACATACCTAAATTCAATTGGAGTAGATTCTAGTTATGGCAACCGAGCTAAGCTTGCCGCTCAATACGGAATCAAAGGTTATGAAGGTTCTGCCGCTCAAAACCTTGCTCTTCTTGAAAAAATGCGTAACGGGGCTAAACCAGTTGCCGCTAAGCCTGCGCCTAAGGGGGATATGAACACAGGCAGTATTGTGACATATCTAAATTCTATCGGTGTAGACGCAAGCTTTAGCAACCGTGAAAAGCTTGCAGCTAAGTACGGTATCCGAGGCTATCAAGGTACAGCAGCTCAAAACACTGCTCTTCTTGAAAAAATGCGTGGCGGTGCAGCACCAGTAGCGGCTAAGCCTGCACCAAGAGGAGATCAAAAGACTACAAGTCTTGTGGACTACCTGAAGTCTATCGGTGAAGGTTCTAGTTTTGCAAACCGTGAAAAGCTTGCTAGACAGTACGGTATCAGCGGATATAAAGGTACTGCTTCTCAAAATACCGCCCTTCTTAAAAAGATGAGAGGGCATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (aMK) rather than this protein.
PDB ID
aMK
Method AlphaFoldv2
Resolution 93.58
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50