Protein

Protein accession
A0A7R7ER35 [UniProt]
Representative
8MrbY
Source
UniProt (cluster: phalp2_1214)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAGEVFSSLITSVNPNPMNAGSRNGISIDRIILHHNATTNKDVAMNTWLLGGGAGTSAHYEVTPTEIIGCVGEQYSAFHAGGTGGIDVPKIANPNQRSIGIENVNSSGAPNWDVDPRTITNCARLVADICKRYGIPCDRQHVLGHNEVTATACPGGMDVDEVVRQAQQFMVGGSNNAVKPEPSKPTPSKPSNNKNKEGVATMYCLYERPINSKTGVLEWNGDAWTVMFCNGVNCRRVSHPDEMKVIEDIYRKNNGKDIPFYSQKEWNKNAPWYNRLETVCPVIGITKKS
Physico‐chemical
properties
protein length:289 AA
molecular weight:31504,2 Da
isoelectric point:7,56
hydropathy:-0,47
Representative Protein Details
Accession
8MrbY
Protein name
8MrbY
Sequence length
422 AA
Molecular weight
46300,85740 Da
Isoelectric point
9,33626
Sequence
MAGEVFSNLITSVNPKLMNSGSRNGIAIDRIVLHHNATTNKDVAMNTWVAGGSAGTSAHYEVTPTEIIGCVGEQYAAWHAGGTGGADIPKMSNPNQRSIGIENLNSSGAPNWSIDPRTVTNCARLVADICKRYGIPCDRQHVLGHNEVTSTACPGGMNVDEVVRQAKQFMAGGNNSAPKPSQTNGRQAFFDIVNVNSGAFNVQGWFIPSKSTKGQSIWLYFMDKATNKEIGRFQGKRVVREDVKKVYPSNPNGAEVGFTVNGLTPQNLMGKEYYFLLRYNNDSKEELYVKDKVFKAPALINRGCLDVIRGDKGQVTLHGWHLSSKRRDTDKHFLFIMDKKTNKEIVRFDVTKSSFKASPDIQKLYDGTIAQRGNCRFAFAHALDAKSPARGKDIYILSRYCSDPLGNTGISDQLQLGGTNKL
Other Proteins in cluster: phalp2_1214
Total (incl. this protein): 36 Avg length: 359,3 Avg pI: 8,37
Protein ID Length (AA) pI
8MrbY 422 9,33626
22d34 421 9,51387
2UKsB 413 7,54278
3lUxs 422 9,38958
68ePK 408 9,46475
7PCSY 418 9,20797
8JpFl 408 9,48744
8s0mH 422 9,41956
8tOL4 408 9,45527
A0A288TXU9 422 9,33626
A0A7S9SWF5 418 9,20797
A0A096XT38 289 7,01964
A0A0E3TBA5 289 7,01964
A0A2Z6FZA5 289 7,56489
A0A2Z6FZW5 289 7,01964
A0A411B7H0 418 9,20784
A0A4D6DTJ6 289 7,56683
A0A4D6DUM0 289 7,01964
A0A5K7QTU6 289 8,03929
A0A679L8V5 422 9,42981
A0A6B9ST50 289 7,56683
A0A6V7BBN5 289 7,54688
A0A6V7BE93 289 7,01964
A0A7G8ZZ10 422 9,42981
A0A7R7ER94 289 7,56489
A0A7R7IEX7 289 7,56489
A0A8D6XV23 422 9,42981
A0A8E7FYF4 422 9,42981
A0A976SXR2 418 9,19927
A0A9E7DU56 289 7,02436
A0A9E7DUH9 422 9,33626
A0AAE9FG02 422 9,38958
A0AAE9JW89 418 9,19927
A0AAE9VJ46 269 6,74596
A0AAE9YI54 253 6,11402
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_10524
86QiV
 42 47,5% 309 9.097E-90
2 phalp2_31265
8lSNT
 54 22,8% 328 4.867E-28
3 phalp2_10050
7qQCo
 2 34,0% 279 1.763E-25

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 8MrbY (422 AA)
Member sequence: A0A7R7ER35 (289 AA)
1 422 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Enterococcus phage phi EF19G
[NCBI] 		2785386 Herelleviridae > Kochikohdavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LC596379 [NCBI]
CDS location
range 7019 -> 7888
strand +
CDS
ATGGCAGGAGAAGTATTTAGTAGCTTGATTACAAGCGTAAATCCTAACCCAATGAACGCAGGTAGCCGTAATGGTATCTCTATTGACCGTATTATTCTACATCATAATGCAACAACAAATAAAGATGTTGCTATGAACACGTGGCTATTAGGTGGTGGTGCAGGTACGTCTGCGCACTATGAAGTCACACCAACAGAAATTATAGGGTGTGTTGGTGAGCAGTATTCAGCATTCCATGCCGGAGGCACAGGTGGTATAGATGTCCCTAAGATTGCTAACCCTAATCAACGCTCAATCGGTATCGAAAATGTAAACTCGTCAGGAGCACCTAACTGGGATGTAGACCCTAGAACAATTACAAATTGTGCCCGTTTAGTGGCAGATATTTGTAAACGTTATGGTATTCCATGTGACCGACAACACGTGTTAGGACATAACGAAGTAACTGCAACAGCATGTCCCGGAGGTATGGATGTAGACGAAGTTGTACGTCAAGCTCAACAATTTATGGTAGGGGGCTCTAACAACGCAGTTAAACCAGAGCCAAGTAAACCTACACCAAGCAAACCAAGTAACAATAAAAATAAAGAAGGAGTGGCAACTATGTATTGTTTATACGAAAGACCTATTAACTCAAAAACAGGAGTGTTAGAGTGGAATGGTGATGCATGGACAGTTATGTTTTGTAATGGAGTAAATTGTCGTAGAGTATCCCACCCAGATGAAATGAAAGTAATTGAGGATATTTACAGAAAAAACAACGGAAAAGACATCCCGTTCTACAGCCAAAAAGAGTGGAATAAAAATGCACCATGGTATAACAGATTAGAGACAGTATGTCCAGTAATAGGTATTACTAAAAAATCTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8MrbY) rather than this protein.
PDB ID
8MrbY
Method AlphaFoldv2
Resolution 91.85
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50