Protein

Protein accession
A0A7G9A1Y0 [UniProt]
Representative
6S4LS
Source
UniProt (cluster: phalp2_35268)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTVTIAYDRTVSNLIDELSATGHVTHRSYKKTSVTLHHNAGRLSHQGVLNVWKTRPASAHFDVDSAGAVAQYVKVNEYAWAAGNTKGNQTSIHIEMANSKLSPTWEVGDATWKGAARLAGWLFYKVIGARPTKDNLFYHSHWAATACAGPYMKKIYDDVLKEAQKAYDSFKKAPSSPSPSTPTKKTVDQLAKEVIAGKWNNGPARVRKLLAAGYDPEKVQARVDELMNPKRGPKSIKTLAAEVLAGKWGDDPSRSRDLKEAGYDPVAVQKEVNRQVRARR
Physico‐chemical
properties
protein length:280 AA
molecular weight:30897,6 Da
isoelectric point:9,77
hydropathy:-0,59
Representative Protein Details
Accession
6S4LS
Protein name
6S4LS
Sequence length
292 AA
Molecular weight
32445,60570 Da
Isoelectric point
9,95851
Sequence
MTATIPYDLPVKNLIDELSATGHVTHTSYVKKSVTLHHNGGRLSHEGCLNVWKTRPASAHFDVDRNGAVCQYVKPNEFAWHAGNTQGNRESIGIEMANSTTAPGWEVAEVTWKAAARLAGWLFAKVIKARPSSSNLFVHSHWRATACAGPYIKSVWSRVLAETQRWYDIFTKPPKPSTPTTAAPKPIQGSTYTGSKLIRTSAVREKYRNPSIARFNGLLWAWLCKNSPDYARKNLAAWNKEPSDKYGLQAQRATQEMYRIMAARKPKEFTKVSLPTWPGDSGVRAVGGVPVK
Other Proteins in cluster: phalp2_35268
Total (incl. this protein): 6 Avg length: 283,0 Avg pI: 9,79
Protein ID Length (AA) pI
6S4LS 292 9,95851
A0A2U8UQJ7 280 9,76865
A0A2U8UQU9 280 9,76865
A0A5J6D7U0 286 9,70315
A0A6M3SVZ4 280 9,76865
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_28151
bt8E
 19 67,7% 189 7.674E-92
2 phalp2_30585
5Zqsx
 178 35,8% 184 5.803E-25
3 phalp2_26331
1cKvm
 65 30,6% 183 8.346E-16
4 phalp2_29544
3KzTO
 225 26,4% 189 1.076E-10
5 phalp2_18388
5HE4u
 2 27,7% 191 3.443E-10
6 phalp2_2602
6IbUl
 53 27,9% 186 1.970E-07
7 phalp2_12971
3esnk
 626 20,6% 194 2.537E-05
8 phalp2_18247
7DM9M
 20 24,3% 226 4.349E-04

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 6S4LS (292 AA)
Member sequence: A0A7G9A1Y0 (280 AA)
1 292 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptomyces phage Thiqqums
[NCBI] 		2759458 Bingvirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MT657340 [NCBI]
CDS location
range 1128 -> 1970
strand +
CDS
GTGACTGTGACCATAGCCTACGACCGCACGGTCTCCAACCTGATCGACGAGCTCAGCGCGACCGGTCACGTCACGCACCGCTCGTACAAGAAGACCTCCGTCACGCTCCACCACAACGCCGGCCGTCTGTCGCACCAGGGTGTTCTGAACGTCTGGAAGACCAGGCCTGCTTCGGCTCACTTCGACGTCGACTCCGCCGGAGCCGTCGCTCAGTACGTCAAGGTCAACGAGTACGCCTGGGCCGCGGGTAACACCAAGGGCAACCAGACCTCGATCCACATCGAGATGGCGAACTCGAAGCTCTCTCCGACGTGGGAGGTCGGTGACGCCACCTGGAAGGGTGCGGCTCGTCTCGCGGGTTGGCTCTTCTACAAGGTCATCGGCGCTCGCCCGACCAAGGACAACCTGTTCTACCACAGCCACTGGGCGGCCACCGCCTGCGCCGGCCCGTACATGAAGAAGATCTACGACGACGTGCTCAAGGAAGCACAGAAGGCGTACGACTCCTTCAAGAAGGCCCCCTCCTCCCCGAGCCCCTCCACCCCCACCAAGAAGACGGTCGACCAGCTCGCCAAGGAAGTCATCGCCGGCAAGTGGAACAATGGTCCGGCCCGAGTCCGCAAGCTTCTTGCGGCGGGCTACGACCCCGAGAAGGTCCAGGCTCGCGTCGACGAGCTGATGAACCCGAAGAGGGGTCCCAAGTCCATCAAGACTCTTGCCGCGGAGGTTCTCGCCGGCAAGTGGGGTGACGACCCGAGCCGTTCCAGGGATCTCAAGGAGGCCGGCTATGACCCTGTTGCCGTCCAGAAGGAGGTCAACAGGCAGGTCAGGGCCCGCCGGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6S4LS) rather than this protein.
PDB ID
6S4LS
Method AlphaFoldv2
Resolution 75.34
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50