Protein

Protein accession
A0A6N3ISM5 [UniProt]
Representative
7qsYO
Source
UniProt (cluster: phalp2_23551)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MIEIWKGVNVRYDLLPIGTRRTGQAMAVKKPVFIVAHDTGNINTTAQNNVDYYKNTYNIDWASTASAHIFVDDKEAIICVPTTEKAWHVLYNAPMDDLWYGINANDGAIGVEICYFSDKERSKKSLDNGARVLAYLAEYWGVDYKTNMPGHQDIQSDKQDPGNLLEACGLGRNTSNLDKEVAKYYKKVIKSVDVKKSGNAEPRVKTQSTKTMWSWKGTFYPDRAIKVRKSPGLKGTIVNRDSWLYDKDDWVEFDQVIKADGYWWIRFKYPKPGSSKNYFYCAVCEITDKQERIKKEKYFGKIDWK
Physico‐chemical
properties
protein length:305 AA
molecular weight:35115,5 Da
isoelectric point:8,97
hydropathy:-0,65
Representative Protein Details
Accession
7qsYO
Protein name
7qsYO
Sequence length
553 AA
Molecular weight
62667,17180 Da
Isoelectric point
8,80021
Sequence
MTELWNGVPVRFDLLPIGTRRTGQPLTTGKPKFAVIHDTGNKNSTAQQNVNYYKNSANISWAYVASAHIFVDDKECIICIPVTEKAWHVLYDTPLDNQSYGLDANDAAFGVEASYFDDKKRSLKSLDNSARVMAYLCKYWGIDYKTQVPGHQDIQYDKIDPGNLLEACGYSRSVANFDKQVAKYINGVKVPLTNKVKPASKKPAGDKKVDKPTPLPKAEMTQSDALKYALSLEGQYIDFDGEFAYQCMDVAVQYVYHITNKKLKLWGNAIDAIKNNFPTGWKIVKNTPDYVPPVASIAVWTEQEYSKWGHIAIVIDNKGGTEYVTVLEQNFDSKANSPVKRRNDDFRGLTHFIVPDFADVTTVETTHKPKPSNSKQYTKIPMNLTWSNTAYFRAQADSAGVAIAKANHNHNMVVTNETYPQGTDFYVFEIVDGWCRVYSPNNDGWVWHERLILKETFKPLDPKKKQASNLTKGATPPLSMSNSPKAKFRAKADKYGVTICKKQGNNFVLTNEVYKPGYDQFYVFEIVDGWCRVYSNTNNGWVWRERLNIVEIY
Other Proteins in cluster: phalp2_23551
Total (incl. this protein): 131 Avg length: 477,6 Avg pI: 8,79
Protein ID Length (AA) pI
7qsYO 553 8,80021
13cR6 577 6,95325
1bCF5 578 5,71092
1bE0S 576 8,65980
1bHcg 576 7,92608
1bMLj 474 9,37140
1bvKx 570 9,17335
1c7ha 576 8,57038
1dOKa 578 9,35045
1dP4s 574 9,58627
1dP6o 569 9,29043
1enSJ 574 9,55868
1enVz 574 9,58640
1jJDF 569 9,16536
1jNPu 569 9,11372
1kl0z 576 7,57422
1nLfJ 470 9,27715
3iUWp 578 9,37166
3kWbS 470 9,34432
4168k 560 9,36540
5Qb4Z 569 9,23015
5S4Vz 574 9,48705
5UkeB 574 9,42039
5XxEi 569 9,04493
69M4g 574 9,51871
69TyM 574 9,51374
6s8Ir 574 9,51374
72BTf 576 8,47142
72lys 570 4,75631
747SN 551 6,54077
75Byf 470 9,32305
75SrT 470 9,24685
7QiTi 569 9,13796
7QkaO 578 9,34568
7RhRr 574 9,48216
7RpEC 570 4,87283
7a2BC 578 9,35045
7bi11 470 9,24685
7c2t9 470 9,33852
7cW9P 577 6,36605
7cWbp 577 6,13608
7e7rA 420 8,43725
7e8Dn 470 9,21970
7eaDj 576 8,65264
7ekTH 470 9,20881
7g7OB 569 9,22099
7gHzR 570 9,09657
7geKY 569 9,22112
7gfYi 578 9,35045
7gihI 569 9,12287
7gmPZ 569 9,19166
7hMTs 569 9,39396
7hOcc 569 9,27515
7hQJT 569 9,37314
7hQQ7 569 9,37320
7ifml 569 9,16445
7ihmD 569 9,19804
7ihwp 569 9,25858
7ij4Y 569 9,30996
7ikGT 570 9,27038
7ikLa 570 9,14241
7ipFx 569 9,24394
7iw5F 569 9,23053
7izcu 570 9,26567
7izdm 569 9,21158
7l1dZ 570 4,88647
7mKUx 569 9,06988
7nu7j 569 9,29533
7owis 577 8,20446
7owjW 570 4,56993
7qP5E 577 6,29852
7qZNR 570 9,14679
7qq5Q 573 8,71550
7qq7G 573 8,84295
7qqar 573 8,71924
7qqfU 573 8,78087
7qtE3 575 8,95899
7r9lJ 552 7,06795
7rdE5 574 5,51608
7rdEf 570 4,77399
7rqJk 570 9,14234
7rqxS 578 9,35528
7rrre 578 9,32343
7u9l8 573 9,01972
7yiVt 574 9,49215
7z9rW 470 9,28856
88WiX 551 5,13560
8JNtS 569 9,07845
8MW61 574 9,49821
8dbuL 384 8,35318
8ftGV 569 9,33865
A0A0U1ZY89 305 8,96854
A0A1X9SIB3 305 8,96854
A0A1W6JPY4 315 6,39793
A0A1P8CMK9 305 8,96190
A0A385IKQ3 492 9,52644
A0A455VCL2 305 8,96854
A0A6N3IKS3 305 8,96854
A0A7G1KWJ0 305 9,47358
A0A7S9SVD7 318 9,21629
A0A7S9XEP0 278 9,89237
A0A7T7K7M1 265 10,02769
A0A7T7K7V3 316 9,43554
A0A7T7Z7S3 305 8,96854
A0A7T8IXK0 320 9,30899
A0A7T8IXK8 316 9,39686
M4I0H0 305 8,96854
S4T909 305 8,96854
A0A2H4IZ35 245 9,53283
A0A2H4J2K9 470 9,24685
A0A8B6MGV7 316 9,39686
A0A977KJS0 305 8,96854
A0AA94X1J2 305 8,96854
A0AAD1NYC3 307 9,53012
A0AAD1PVN2 305 8,96854
A0AAE9VUH4 305 8,96854
A0AAU8GSD7 293 9,71443
A0AAX4B4H4 319 9,48009
A0AAX4B5A9 319 9,47158
A0AAX4B5X1 319 9,48009
A0AAX4B6E4 319 9,48009
A0AAX4NTJ4 300 9,47932
A0A977TH64 250 9,36083
A0A9E6XRT5 250 9,30010
A0AAE7FLC1 245 9,40221
A0AAE7KCD7 245 9,53283
A0AAF0IDQ6 245 9,53283
A0AAX4BIF2 245 9,37185
A0AAX4BJ14 245 9,37185
A0AAX4J7M3 111 7,77332
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_10314
1lVmq
 17 65,0% 343 1.511E-180
2 phalp2_36985
8MohX
 8 47,0% 378 1.604E-149
3 phalp2_8239
7wAa9
 4 36,1% 523 2.706E-106
4 phalp2_37223
23Tki
 1 23,8% 427 7.068E-16

Domains

Domains [InterPro]
Ami2
CHAP
Unannotated
Unannotated
Representative sequence (used for alignment): 7qsYO (553 AA)
Member sequence: A0A6N3ISM5 (305 AA)
1 553 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage KSAP11
[NCBI] 		2599602 Herelleviridae > Silviavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LC492752 [NCBI]
CDS location
range 130486 -> 131403
strand -
CDS
ATGATAGAAATTTGGAAAGGTGTAAATGTTAGATATGATTTACTGCCTATTGGAACAAGACGCACAGGTCAAGCTATGGCGGTTAAAAAACCCGTTTTTATTGTAGCTCATGATACAGGGAATATTAATACTACAGCTCAAAATAATGTAGATTATTACAAAAATACATATAACATTGATTGGGCTTCCACTGCATCCGCACATATCTTTGTAGATGATAAAGAAGCTATCATATGTGTTCCTACTACAGAAAAAGCTTGGCATGTGTTGTATAACGCTCCTATGGATGACCTATGGTACGGAATTAACGCTAATGATGGTGCTATTGGTGTAGAAATTTGTTACTTTAGTGATAAAGAACGTTCTAAAAAATCATTAGACAATGGAGCTCGTGTCTTAGCTTATTTAGCTGAATACTGGGGTGTTGATTACAAAACAAATATGCCAGGTCACCAAGACATCCAAAGCGACAAACAAGACCCAGGTAACTTATTAGAAGCATGTGGATTAGGTAGAAACACTAGTAACTTAGATAAGGAAGTAGCTAAGTATTACAAGAAAGTTATTAAGTCAGTAGATGTAAAGAAATCAGGTAATGCTGAGCCTAGAGTAAAAACACAATCTACTAAAACTATGTGGAGCTGGAAAGGTACTTTTTATCCAGATAGAGCTATTAAAGTTAGAAAATCTCCAGGACTTAAAGGCACTATAGTAAATAGAGATTCATGGCTATACGATAAGGATGATTGGGTTGAGTTTGACCAAGTTATTAAAGCAGATGGCTATTGGTGGATTCGATTTAAATATCCAAAACCAGGCTCTAGTAAAAATTATTTCTATTGTGCTGTATGTGAGATAACAGATAAACAAGAACGTATTAAGAAAGAAAAATATTTTGGAAAAATAGATTGGAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7qsYO) rather than this protein.
PDB ID
7qsYO
Method AlphaFoldv2
Resolution 79.98
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50