Protein

Protein accession
A0A6M5CA00 [UniProt]
Representative
78x1H
Source
UniProt (cluster: phalp2_3935)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVALNYEQKHIHVNKFSRPGFKLLKVAATVIHYTANNGGTADNHFSYFDTLTDRYASAHIFVDKNKALEIIPLDEVAFHANERKEGPLIPSLKATAPYYGGGNANLISIGIEMCMEKDGMIHPDTITRTILVVQMLQQKFGKQPIYRHYDVTGKNCPAPFVSDKSKWEKFLADVEKKEENQVAEEQKLTPAQEAVRQEAIRLKITDGKDPFRAVNQFYGWALAIPQAQKIEALEKRIVELEKNK
Physico‐chemical
properties
protein length:244 AA
molecular weight:27657,4 Da
isoelectric point:8,35
hydropathy:-0,45
Representative Protein Details
Accession
78x1H
Protein name
78x1H
Sequence length
316 AA
Molecular weight
35567,05800 Da
Isoelectric point
9,41221
Sequence
MSVITNDYIRVNQHSRPGSKLTVVNKIIMHYTANNGGTARNHWGYFNNLTDRYASAHIFVDDIEALCIIPQNEVAYQANDIQKYVNGSPYRGVTALLSNANFKAIGIEMCLDKKGNITAATFNRTVDVAAELCKIHKLKAADIVRHYDVTGKICPAPWVNNSAEFTRFKNAVALKLSGNPKKGKQWLYAKRNLYILKGAGDWTSALAFTLPQHSAVQVDWDNLKNGWFEINYQGKKGYYSASVANYFDEKNPNVTYICQDDLLFRADPKWGGPPSFRRKKGETINAVEKLSNGWLKCTLGTQYGYLPDSTTYLKKK
Other Proteins in cluster: phalp2_3935
Total (incl. this protein): 58 Avg length: 361,6 Avg pI: 9,68
Protein ID Length (AA) pI
78x1H 316 9,41221
1GVPI 354 9,55720
1aZYs 320 9,51768
1dFRU 320 9,43097
1jLuv 322 9,46939
1kkeV 320 9,35328
3n27D 354 9,65661
71VKT 385 9,75763
72jVY 387 9,92202
74ct2 385 9,67975
75OFK 387 9,83409
75OYD 354 9,62141
75jIg 261 9,45263
75jOm 289 9,66989
75qOh 384 9,70231
78Ojq 385 9,81559
79HPO 354 9,53818
79Jr0 385 9,71308
7C2li 387 9,83447
7C2m6 385 9,67891
7bcWc 385 9,78245
7cDhc 386 9,66924
7dRB2 387 9,86400
7dZLY 385 9,70212
7fNmS 386 9,72449
7guLn 386 9,79018
7hiXD 385 9,80798
7hidG 354 9,68040
7hjnA 385 9,83518
7i8Xw 386 9,67975
7knDi 385 9,70212
7knys 386 9,71308
7l8rL 386 9,74834
7lBjI 354 9,68040
7pex6 343 10,23450
7qVvw 319 9,83808
7qqDr 295 9,36263
7qqG2 295 9,52844
7qyRM 385 9,71308
7u60D 354 9,56745
7u63W 387 9,89301
7uMWQ 386 9,74602
7ugCP 386 9,80978
7ulXb 385 9,79470
7um0O 386 9,67994
7v1Ae 385 9,64552
7v1Hn 359 9,79612
7v1uz 387 9,84692
7wNnC 318 9,38880
7xMvV 387 9,90906
7xN1S 387 9,86400
7y8DA 386 9,70212
7y8tp 387 9,86374
7yCxj 384 9,65861
7yTT3 319 9,64545
9xv 354 9,63172
hee 387 9,90597
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_14020
81gAb
 48 40,2% 318 5.304E-88
2 phalp2_37528
3iFdV
 13 49,5% 202 4.036E-71
3 phalp2_11225
6wIAx
 104 28,4% 320 2.919E-34
4 phalp2_11676
1LRu7
 24 30,6% 248 2.919E-34
5 phalp2_3987
7ylun
 65 29,8% 218 7.332E-34
6 phalp2_10049
7q2PM
 57 29,5% 254 5.361E-32
7 phalp2_12878
2omQf
 1 28,6% 335 7.139E-30
8 phalp2_14662
5ObrM
 161 28,5% 298 9.354E-28
9 phalp2_15067
7vQ2g
 6 25,9% 289 1.515E-23
10 phalp2_26828
3TOBT
 44 29,8% 211 2.276E-22

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Representative sequence (used for alignment): 78x1H (316 AA)
Member sequence: A0A6M5CA00 (244 AA)
1 316 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Psychrobacillus phage Spoks
[NCBI] 		2736239 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MT410774 [NCBI]
CDS location
range 18318 -> 19052
strand +
CDS
ATGGTCGCATTAAACTACGAGCAAAAGCATATTCACGTAAATAAATTTAGTAGACCAGGTTTCAAACTTCTTAAAGTTGCAGCTACAGTTATCCATTACACTGCAAATAACGGTGGAACTGCGGACAACCATTTTAGTTATTTCGATACATTAACAGATCGTTATGCATCTGCACATATTTTCGTAGACAAAAATAAAGCGCTTGAAATTATTCCACTAGATGAAGTTGCCTTCCACGCAAATGAACGAAAAGAAGGACCATTAATTCCTTCACTCAAAGCAACAGCTCCTTATTATGGTGGAGGTAATGCTAATTTAATCTCCATCGGAATTGAAATGTGCATGGAGAAAGATGGAATGATTCATCCAGATACTATCACAAGAACGATTTTGGTCGTTCAAATGCTGCAACAAAAGTTTGGTAAACAGCCTATCTATCGACACTACGACGTGACTGGAAAGAATTGCCCAGCGCCGTTTGTGAGTGATAAAAGTAAGTGGGAAAAGTTTTTAGCAGATGTAGAAAAAAAGGAGGAAAACCAAGTGGCAGAAGAACAAAAATTAACACCAGCACAAGAAGCGGTAAGACAAGAAGCAATCCGATTAAAAATCACAGATGGCAAAGATCCTTTCCGAGCAGTCAATCAGTTTTACGGGTGGGCTTTAGCAATACCGCAAGCACAAAAAATTGAGGCGTTAGAAAAGCGCATTGTCGAGTTAGAAAAAAATAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (78x1H) rather than this protein.
PDB ID
78x1H
Method AlphaFoldv2
Resolution 95.34
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50