Protein

Protein accession
A0A6M3SVJ2 [UniProt]
Representative
bt8E
Source
UniProt (cluster: phalp2_28151)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MATIAYDQHVTDFIAALSATGHVTHTRYKKTSVTFHHNGGNLTHSGVLSVWKTRPASAHFDVDAFGRVAQYVDVREYAWAVGNRGGNESTISIEMADKTFSPHWEVAEDTWKSAARLAGWLFAHVIKATPTKNNVHYHHDWSATECPGPYMDSIRAELLAEVQKWYAHFTDDEKGSSPSSPRPSTPAPTKSYTEVARDVIAGKYGNGDTRVKRLKEAGYNPTTVQAEVNRLLSGTTKPARKSINEIAHEVIDGKWGNGADRVTRLTKAGYNSNTVQKEVNRLLS
Physico‐chemical
properties
protein length:284 AA
molecular weight:31407,6 Da
isoelectric point:8,98
hydropathy:-0,59
Representative Protein Details
Accession
bt8E
Protein name
bt8E
Sequence length
233 AA
Molecular weight
27099,99200 Da
Isoelectric point
6,54543
Sequence
MAKTINYDKHVKNLIDELSNTGHVYHTKYKKTSITFHHNGGNLSFEDVLRTWTFKKASAHFDSDEFGNIAQYVNAEEYAWAVGNTEGNKSSISIEMANKTGAPHWEVGEATWESAARLAGWLFAHVIEEEPTHRNVLLHDHWSATECPGPYVHSIYKQLLAEVQRWYRHFHKLIQEKHSKEEPHHDELQKHYTKSEEIIRTIQDLMDVKVDGKWGPKTDEAVLAFRKKHLNKF
Other Proteins in cluster: phalp2_28151
Total (incl. this protein): 19 Avg length: 248,9 Avg pI: 8,17
Protein ID Length (AA) pI
bt8E 233 6,54543
1eDYS 276 9,66763
1hJeN 256 7,23926
1hQtD 302 7,71995
1hTXl 256 7,23926
1osPa 204 9,85195
40vS0 291 9,88927
4MS0d 257 7,98952
4TpOK 181 9,47036
4XRvR 252 7,96038
6ERXB 183 9,44837
6RY5h 180 6,39566
6SLv0 250 7,26086
6SYHz 252 8,52602
6SYsT 254 8,85997
6wTvO 261 9,65616
89XII 304 6,87311
fMGK 253 5,63646
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_35268
6S4LS
 6 52,9% 187 3.129E-96
2 phalp2_4921
6qvcZ
 45 31,7% 192 1.375E-31
3 phalp2_30585
5Zqsx
 178 29,3% 201 4.651E-26
4 phalp2_29544
3KzTO
 225 26,4% 208 1.353E-14
5 phalp2_33602
lAxW
 88 25,9% 231 2.481E-14
6 phalp2_4005
10KQf
 1 23,0% 226 4.545E-14
7 phalp2_28305
13CeQ
 161 28,8% 215 1.258E-12
8 phalp2_30111
3bZzl
 4 25,3% 162 2.535E-11
9 phalp2_2602
6IbUl
 53 25,6% 207 3.733E-10
10 phalp2_32194
6VJSL
 13 27,3% 161 4.029E-09

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): bt8E (233 AA)
Member sequence: A0A6M3SVJ2 (284 AA)
1 233 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptomyces phage ClubPenguin
[NCBI] 		2725618 Bingvirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MT310852 [NCBI]
CDS location
range 874 -> 1728
strand +
CDS
ATGGCGACCATCGCTTACGACCAGCACGTCACCGACTTCATCGCCGCGCTCAGCGCGACCGGTCACGTCACCCACACGAGGTACAAGAAGACCTCCGTCACGTTCCACCACAACGGCGGCAACCTCACCCACTCCGGCGTCCTGTCCGTCTGGAAGACACGCCCGGCTTCCGCCCACTTCGATGTCGACGCCTTCGGCCGAGTCGCCCAGTACGTCGACGTCCGCGAGTACGCCTGGGCCGTCGGCAACCGCGGCGGCAACGAGTCCACCATTTCCATCGAGATGGCAGACAAGACCTTCTCGCCGCACTGGGAGGTCGCCGAGGACACGTGGAAGTCGGCCGCTCGTCTCGCGGGCTGGCTGTTCGCTCACGTGATCAAGGCGACGCCCACCAAGAACAACGTGCACTACCACCACGACTGGTCGGCGACCGAGTGCCCCGGCCCGTACATGGACTCGATCCGCGCCGAGCTCCTCGCCGAGGTCCAGAAGTGGTACGCGCACTTCACCGACGACGAGAAGGGCTCTTCCCCCTCCTCCCCGCGCCCCTCCACCCCCGCACCGACCAAGTCCTACACCGAGGTCGCTCGGGACGTCATCGCTGGGAAGTACGGAAACGGCGACACCCGTGTCAAGAGGCTGAAGGAGGCCGGGTACAACCCCACGACCGTCCAGGCCGAGGTGAACCGCCTGCTCTCCGGCACGACCAAGCCCGCCCGCAAGTCGATCAACGAGATCGCCCACGAGGTCATCGACGGCAAGTGGGGCAACGGCGCCGACCGGGTCACCCGTCTGACCAAGGCCGGGTACAACTCCAACACCGTTCAGAAGGAAGTCAACCGACTCCTCAGCTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (bt8E) rather than this protein.
PDB ID
bt8E
Method AlphaFoldv2
Resolution 93.80
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50