Protein

Protein accession
A0A6B9J1E4 [UniProt]
Representative
6F3wF
Source
UniProt (cluster: phalp2_36096)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
VAL
Probability: 94% (predicted by ML model)
Protein sequence
MQIFNDKLVGEGIAFEKTSKIGNGVNKRKFIVVHYTAGGSYVNDVKTLSSSPKQVSCHLVIGRDKDQITQIGDFNDVQWHAGASQWKGINGLNSYSIGIELTNPGWMELSADKKTATTYYGKVYDVEVSKLILARHSALGDKTYAWLPYTQSQIQNLMDIVEVLKETYPSIQEVVGHEQISPGRKQDPGIGIIFPQKLLDKLNETNMDGSDHFYEGTDEAVHLNRDYKEASESAGLYQVKTAGTALRIRDNYNSTANVIGSLNNSDLVRVERIVGGFAKLIARKSGPSIGPDDGYKTIDGYASTVYLKKVD
Physico‐chemical
properties
protein length:311 AA
molecular weight:34319,2 Da
isoelectric point:6,40
hydropathy:-0,42
Representative Protein Details
Accession
6F3wF
Protein name
6F3wF
Sequence length
308 AA
Molecular weight
33649,77030 Da
Isoelectric point
6,73379
Sequence
VRYEEFTIKKGIGYCNGVALPFKRSPNIGGSLKPRYIVIHDTASGLKDDGDIAWLTDKASKVSAHVVVSREGKVTQLVPFNVVAWHAGQSQWKGLKFLNSYAVGIEIDNPGKLQKVSEGVYKNDIVTIDTNKNPSLKVSYAKTSAHGAGYWLEYTSEQIETVKRLCTALCVEYRIEDILTHWMISPGRKIDTNPTYPLDKLRAAALGSSGGVPLGFMTEAQSEDGERTDTDESGEKFDEDHRNEKSDESHIGAFAKVKQFIKGKIAAGTGIFGGLSLSSLSGVDWKVIAVVGAIAVIGLAIWIWSERE
Other Proteins in cluster: phalp2_36096
Total (incl. this protein): 69 Avg length: 283,8 Avg pI: 7,34
Protein ID Length (AA) pI
6F3wF 308 6,73379
2Rrff 291 7,72870
2WU6x 272 6,50473
2cl1t 265 9,29649
2jVR1 263 6,50991
2qP3 277 9,62579
2tvhk 280 6,60045
3FqN 262 5,84239
3Irr 267 6,92921
3Pr9c 265 8,61383
3aShd 262 7,17299
3gvSx 262 6,75176
3m2Ti 268 7,10939
48mMy 300 7,15492
4KKZs 277 9,46011
4Khfh 265 6,79660
4UcE6 263 6,49001
4UqKs 269 8,46871
4Wnfx 280 8,85501
4cf6u 303 8,35796
4nZJ1 279 8,41830
4qGKz 301 8,72794
4uHoc 267 7,17646
4uO4x 241 8,53215
4uOPj 270 6,24913
4v33b 293 7,23403
5Hnme 282 8,88724
5kmMQ 285 9,24285
5sIJs 234 6,71356
6Dwse 303 8,40121
6N1aN 265 6,54452
6RXnQ 278 8,80034
6TnMY 277 9,58318
6VdUo 265 6,19502
6Vedf 240 6,16472
71YXd 303 7,74399
7Re4A 302 6,51082
7Xo9E 311 8,42900
7YROy 292 7,20891
7b7Oj 304 6,24248
7dDbf 302 7,01538
7lLWN 304 7,02657
7m6El 270 6,65990
7p4Qy 285 7,17759
7p4Re 285 6,83485
7vVpk 284 6,55657
7ymru 268 9,05396
8Isq2 263 6,13096
8Isq3 280 9,13957
8fnwS 263 6,79734
BW8A 265 6,92932
Bi0L 289 9,06730
E0Jq 277 9,54224
InQW 292 7,91422
QB2D 322 5,95010
jsUF 263 7,92009
toPN 262 5,90531
vdQL 281 7,81939
A0A076YN96 311 6,00501
A0A6B9J1R6 311 6,61222
A0A7G7WW08 311 6,00501
A0A7G7WX73 311 6,18752
A0A7S5R0P9 314 6,18433
A0A7S5REQ6 314 6,18433
A0A7S5US75 315 6,66479
A0A7S5UWM2 314 6,18433
A0A7S5UX47 314 6,18433
A0A7S5UZU0 310 6,24646
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_4783
4YJpR
 19 38,4% 208 6.413E-81
2 phalp2_31152
3LHkO
 133 45,7% 190 5.127E-76
3 phalp2_22919
33qi6
 180 44,9% 207 2.130E-68
4 phalp2_40199
2cSYZ
 15 35,3% 195 3.905E-42
5 phalp2_6728
24ioh
 33 27,7% 191 3.140E-36
6 phalp2_39713
f1Qc
 4 25,2% 226 2.343E-23
7 phalp2_0
4IvO2
 7 24,2% 202 1.595E-21
8 phalp2_5683
4DyjT
 1 24,1% 195 5.924E-16
9 phalp2_20417
3dS44
 3 23,3% 206 1.930E-15
10 phalp2_13794
6WRQm
 7 24,4% 209 3.480E-15

Domains

Domains [InterPro]
Ami2
Disordered region
Representative sequence (used for alignment): 6F3wF (308 AA)
Member sequence: A0A6B9J1E4 (311 AA)
1 308 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Rhizobium phage RL38J1
[NCBI] 		2663232 Pootjesviridae > Innesvirus > Innesvirus RL38J1
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MN549360 [NCBI]
CDS location
range 69901 -> 70836
strand -
CDS
TTGCAAATCTTTAACGACAAGCTCGTAGGCGAAGGCATCGCTTTCGAGAAAACTTCGAAGATCGGAAACGGCGTGAACAAGCGAAAGTTCATCGTCGTGCACTACACTGCCGGCGGAAGCTACGTGAACGATGTGAAGACGTTGTCATCGTCTCCGAAGCAAGTGAGCTGTCATCTGGTTATCGGTCGCGACAAGGATCAGATCACCCAGATCGGCGACTTCAACGATGTTCAGTGGCATGCTGGTGCATCGCAGTGGAAAGGCATCAACGGTCTCAACAGCTACTCGATCGGCATCGAGCTGACAAATCCTGGCTGGATGGAGCTCTCGGCTGACAAGAAGACTGCAACGACGTACTACGGCAAGGTGTACGATGTCGAAGTGAGCAAGCTCATTCTCGCAAGGCATTCTGCTCTCGGCGACAAAACTTACGCATGGCTTCCGTACACTCAGTCGCAGATCCAGAACCTCATGGACATCGTCGAAGTTCTGAAGGAAACGTATCCATCGATCCAGGAAGTCGTCGGCCACGAGCAGATCTCTCCTGGTCGCAAGCAGGATCCAGGCATCGGAATCATCTTTCCGCAGAAGCTCCTGGACAAGCTGAACGAGACGAACATGGACGGCTCTGATCACTTCTACGAAGGAACCGACGAAGCGGTCCATCTGAACCGTGACTACAAGGAGGCATCCGAGTCGGCTGGTCTGTATCAAGTCAAGACGGCTGGAACAGCTCTTCGTATCCGAGACAATTACAACTCGACAGCTAACGTCATCGGAAGTCTGAACAACAGTGATCTCGTCCGAGTTGAACGGATCGTAGGCGGTTTCGCTAAGCTTATTGCGAGGAAGTCAGGTCCGTCCATCGGTCCTGACGACGGATACAAGACGATCGACGGATACGCATCCACTGTGTATCTCAAGAAAGTTGACTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6F3wF) rather than this protein.
PDB ID
6F3wF
Method AlphaFoldv2
Resolution 80.49
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50