Protein

Protein accession
A0A5J6TEW0 [UniProt]
Representative
7RbAk
Source
UniProt (cluster: phalp2_40628)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSFIRSKFAPRALLTVDQWIAIFVAVADELDMPDKRGAVVCAAMCAFQEAGADLQDGNGRQIWIPGNMADPCYADDPDAYPHDSEGNDGQSTGPFQQQMNQPGKAPWGWGGNYGDCAGTRKRMDPWDSTRMFFGWPGSGLRDKGYDASTAQRANDSIQRVQGSGVPNAYAQWWGLANAAYDRYLGNPIPAPPSPGGGSSGAPALTPNPAWRGDPLFLPQLLRAFGVSVTTYTDADGIRWDERGHGDFGKISWVLWHHTGSVNETDNGIAHHPALGLAANMLIHPDGHVVLTGSGIAWHGGIGVYPGIPEDGINQISIGIECSYGPDRDGRYTLPWPEAQMNAMIAVGGAISWFLGDTLPPSHQIAHKEWAGADNPLGVNKQGKPDPGNLDMTWFRAQIAARAAAGPTTGGDDWMTNPDAITMLAAIYRETVTQKSPSRSFMAEDGTLIDTPLGIDWNTDGNAWTLVMTEAYWNDVPLAITVVEDIAANGVRQTSWAGSADKDDQVKWNQWLRDFGQAYCAGLVRRKAQWTALVAAVSALAAAQSAAAVTAEATTPAAPKKRAPRKRAATKPTTTGGAE
Physico‐chemical
properties
protein length:578 AA
molecular weight:61880,3 Da
isoelectric point:5,13
hydropathy:-0,37
Representative Protein Details
Accession
7RbAk
Protein name
7RbAk
Sequence length
512 AA
Molecular weight
55357,38050 Da
Isoelectric point
5,36653
Sequence
MSFVWFRPDGPLRSREQIACEVHAVSLARGLDELATVLALMCIDVEVGAADDNGERQWWCPWNAADPQTEQFDHDSQSNDALSSGYFQQQVSRPGAPGRPWGWGGLFGDLNGARKRMTLADSADMFLAALPDDYGRAAGNPAVAGQVVQQVQKSAFPDRYAQRWGEAWSVLRRALAGGPVDPSVPTTPDVLTPAPGFRGDPYWLADVLRAEGLRVFEMDGWKDRGEGDQGVLWGAVFHHTGNANETPEGIAFHPTLGLAAHLLIRPNGDVWVCGIGKANHAGVGSWPGIPTDNANPVTIGVEVAILPQENAPHRTGWPPVQYEATVKAFAAILRKLAQTAKRAISHKEWAQLGPAGVRQGKWDPGAIDMNIFRTDVQRQIDTRTTGGFLMALTDSEQREILDYVRAQNAPIPSTSPLRHLGEGNVNTRANLARAIDANQHVTAVVTLAKEGHTPSIALLWEVSTAADNPGTYPDRQEDANLAKTLLASISKTKKAVAAEDIEAWLDAEKAAA
Other Proteins in cluster: phalp2_40628
Total (incl. this protein): 146 Avg length: 504,8 Avg pI: 5,28
Protein ID Length (AA) pI
7RbAk 512 5,36653
1pDWd 433 4,96911
232jx 531 4,98833
3376W 523 5,41479
3NQZM 433 5,08609
4nQGA 577 5,25933
5J48n 408 6,11101
5kJQX 428 5,56706
6PhMz 406 5,13929
6PhYT 433 5,04579
728bX 499 5,09973
72J0i 499 5,20375
72Jkc 496 5,20375
75mYG 500 5,14708
75n2J 499 5,31663
76nsx 433 4,92973
7AIGY 578 5,19437
7ApKJ 510 5,77498
7ApQT 432 5,74531
7AseY 528 5,68301
7B5Kz 454 5,80999
7B5i9 570 5,03118
7BsA2 577 5,31350
7PTJI 573 5,06784
7PUM4 476 5,87729
7Q0JU 577 5,33737
7Q0iN 428 5,74525
7cEeH 451 5,12195
7f3tR 499 5,47612
7gyDB 486 5,27354
7hKmd 433 5,07262
7hU8m 433 4,93001
7hUbd 499 5,32640
7hwIo 476 4,95957
7jHYW 552 5,61589
7kFzv 499 5,42133
7lbK7 506 5,15384
7ogvs 726 5,77430
7ogw8 424 5,15629
7owdv 462 5,76242
7pHgM 505 5,44929
7pHq8 486 5,33016
7pHsU 433 5,09570
7pIQc 499 5,39012
7pJSq 433 4,97105
7pKZr 499 5,20915
7pLUV 512 5,25530
7pObD 499 5,14594
7pP3C 499 5,19948
7pPdl 433 4,96917
7pQ2z 433 4,88153
7pQ75 433 4,98781
7pQQA 505 5,51193
7pQX7 501 5,42008
7pQeh 499 5,20915
7pR6S 499 5,20375
7pRcY 500 5,25763
7pSdq 499 5,33664
7pSjd 499 5,26786
7pTF0 500 5,21449
7pTFf 512 5,30748
7pTw9 433 5,03607
7pVD2 480 5,14134
7pVNl 500 5,27229
7pVkX 433 5,14645
7pWur 499 5,14594
7pWvH 433 4,98628
7pX7V 486 5,27860
7pXzS 433 5,02533
7q42x 433 4,88153
7q4U6 512 5,24171
7q5G0 394 5,09712
7q7YY 512 5,52227
7q82z 512 5,24171
7q8Bc 499 5,25638
7qJW0 427 4,90773
7qK4f 428 5,30594
7qaQB 500 5,24666
7qbKd 499 5,34164
7qbod 499 5,20494
7qfza 499 5,20915
7qgDX 486 5,16561
7qgJt 406 4,92558
7qge2 433 4,93001
7qhn9 512 5,52227
7qiVF 500 5,14708
7qiw2 501 4,93331
7qjPv 499 5,38660
7qlQA 499 5,26786
7qm0h 500 5,69984
7qm1X 512 5,29543
7qmXZ 499 5,26257
7qmmR 433 5,03073
7qxO5 455 5,27911
7r3Wi 428 5,10968
7rirh 508 5,01595
7tLmX 454 5,29242
7ulkF 500 5,02885
8KNEG 551 5,40189
8KNek 521 5,13418
8KQA7 547 5,55427
8KwI0 578 5,18840
8LDXE 574 5,13275
8LbZF 565 5,43429
8LdAc 578 5,25604
8LzGJ 476 5,87735
8MzDp 432 5,75042
kal0 494 5,85796
A0A1D8EYF9 578 5,19437
Q1A072 570 5,03118
B3VLX1 577 5,31350
T2A7Y9 573 5,06784
Q19Y11 502 5,93288
V5UQ97 578 5,18840
A0A7G8LFT5 574 5,13275
A0A0F6WE00 565 5,43429
A0A0K1Y763 578 5,25604
A0A0B5A4W2 502 5,93288
A0A2I2MPE9 502 5,93288
A0A345KWK5 578 5,25604
A0A3G3M0R1 436 6,08094
A0A481VQV4 578 5,26172
A0A5P8DD71 578 5,13275
A0A5Q2WMP7 578 5,13275
A0A6M3T1S5 580 5,19437
A0A7G9W354 578 5,13275
A0A7L7SQA7 578 5,07620
A0A7T1X3A9 499 5,47458
A0A7T3KBQ3 531 5,52477
A0A7U0J7U8 578 5,13275
G1BLA0 578 5,18840
G1BPF4 578 5,07620
T2A8A7 578 5,19437
T2A9T3 578 5,19437
A0A9E7QCZ5 578 5,19437
A0AA49BPE5 537 5,35886
A0AA96JYZ1 578 5,19437
A0AAU8GSX1 578 5,13275
A0AB39AKL1 543 5,17402
A0A899IUC5 500 5,20915
A0A899IWC1 512 5,36653
A0A899IWJ2 499 5,56115
A0A899IWS8 512 5,36653
A0A899J0W7 499 5,18954
A0A899J1Z5 500 5,20915
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_12317
7uhSc
 56 41,7% 419 7.688E-127
2 phalp2_17757
7wVIM
 110 41,6% 329 9.398E-95
3 phalp2_36228
7u9Qz
 32 28,6% 527 2.245E-67
4 phalp2_39054
7o3xD
 152 24,1% 473 4.538E-30
5 phalp2_6665
1IZIA
 9 25,9% 471 6.066E-30

Domains

Domains [InterPro]
Unannotated
Ami2
Unannotated
Representative sequence (used for alignment): 7RbAk (512 AA)
Member sequence: A0A5J6TEW0 (578 AA)
1 512 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Mycobacterium phage Magpie
[NCBI] 		2599869 Coopervirus > Coopervirus bane1
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MN234171 [NCBI]
CDS location
range 40402 -> 42138
strand +
CDS
GTGAGCTTCATCCGGTCGAAATTCGCACCCCGTGCCCTGCTGACCGTCGACCAGTGGATCGCCATTTTCGTCGCGGTGGCCGACGAGCTGGACATGCCGGACAAGCGCGGCGCGGTGGTCTGCGCAGCCATGTGCGCATTCCAGGAGGCCGGGGCCGACCTGCAAGACGGCAACGGGCGTCAAATCTGGATCCCCGGCAACATGGCCGACCCCTGCTACGCCGACGACCCCGACGCCTACCCGCACGACAGCGAGGGCAACGACGGGCAGTCCACCGGCCCGTTCCAGCAGCAGATGAACCAGCCGGGGAAAGCCCCGTGGGGCTGGGGCGGCAACTACGGCGATTGTGCCGGTACCCGCAAGCGCATGGACCCATGGGACAGCACGCGCATGTTCTTCGGCTGGCCGGGCAGCGGGCTGCGCGACAAGGGCTACGACGCCAGCACCGCCCAGCGCGCCAACGACAGCATTCAGCGCGTGCAGGGCAGCGGCGTGCCCAACGCTTATGCGCAGTGGTGGGGCCTGGCCAACGCGGCGTACGACCGCTACCTGGGCAACCCGATCCCGGCACCGCCCAGCCCCGGCGGGGGCAGCAGCGGCGCACCGGCCCTGACGCCGAACCCGGCCTGGCGTGGTGACCCGCTGTTCCTGCCGCAGCTGCTGCGGGCGTTCGGGGTGAGCGTCACCACCTACACCGACGCCGACGGCATCCGCTGGGATGAGCGCGGGCATGGCGACTTCGGGAAAATCTCGTGGGTGCTGTGGCACCACACCGGCAGCGTCAACGAGACCGACAACGGCATTGCGCACCATCCGGCGCTGGGCCTGGCCGCGAACATGCTCATCCATCCCGACGGGCACGTGGTGCTCACCGGCAGCGGCATCGCCTGGCACGGCGGCATCGGGGTGTATCCCGGCATCCCAGAGGACGGTATCAACCAGATCAGCATCGGCATTGAGTGCAGCTACGGACCAGACCGCGACGGCCGGTACACGCTGCCCTGGCCCGAGGCGCAGATGAACGCCATGATTGCCGTGGGCGGTGCCATCAGCTGGTTCCTGGGCGACACGCTGCCGCCGAGCCACCAGATCGCTCACAAGGAATGGGCCGGTGCCGACAACCCGCTGGGCGTGAACAAGCAGGGCAAGCCCGACCCCGGCAATCTCGACATGACCTGGTTCCGCGCGCAGATCGCCGCGCGGGCAGCAGCTGGCCCCACAACCGGAGGAGACGACTGGATGACGAACCCCGACGCCATCACCATGCTGGCGGCAATCTACCGCGAGACCGTGACGCAGAAGTCCCCGAGCCGGTCGTTCATGGCCGAGGACGGCACGCTGATCGACACCCCGCTGGGCATTGACTGGAACACCGACGGCAACGCCTGGACCCTGGTCATGACCGAGGCGTACTGGAACGACGTGCCGCTGGCCATCACCGTGGTGGAGGACATCGCCGCCAACGGCGTGCGGCAGACCAGCTGGGCCGGTAGCGCCGACAAGGACGACCAGGTGAAGTGGAACCAGTGGCTGAGGGACTTCGGCCAGGCGTACTGCGCGGGCCTGGTCCGGCGTAAGGCCCAGTGGACCGCTCTGGTGGCCGCAGTTTCGGCCCTGGCTGCCGCACAGAGCGCCGCTGCGGTCACCGCCGAGGCAACCACCCCGGCCGCGCCCAAGAAGCGCGCACCGCGCAAGAGGGCGGCAACCAAGCCCACCACGACCGGCGGCGCAGAATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7RbAk) rather than this protein.
PDB ID
7RbAk
Method AlphaFoldv2
Resolution 87.99
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50