Protein

Protein accession
A0A4D6AWQ3 [UniProt]
Representative
5Xg2q
Source
UniProt (cluster: phalp2_11189)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MATVDEVIADVDSFVGRCLDMDGYWGGQCMDVIERVASHFGKHLGGNAIDGLTNAQRLGWPVIMNAWGVNPKRGDVGVMSVPGHGFGHIFLVTKDSDGVTIETLEQNIDGYADNNKDGINDQLQVGGVVRRHKRDFTGVIGWFRPPYDTMKTGGKTMSIPTKTVNGDLFSGLVTDVDPNIMNGDYNRTKIDRIVIHHNAGTSDSAARRTWYISTGVGTSAHYQVTPDKIWGCVSETSVAYHAGDYAVNQRSIGIEHLNNAGSPHWTIAEETYHNSAKLIADICQRYNIPIDDKHIIPHRVVSATACPGGIDMAKLIHIAQEVASGAISKTEITVKPSGTFRVKISVSDLNIRKAPSVSSKSLGFIKPGVYTIVETKQVEGYEWGQLKSGIGWIALNYTKKL
Physico‐chemical
properties
protein length:401 AA
molecular weight:43679,9 Da
isoelectric point:6,73
hydropathy:-0,24
Representative Protein Details
Accession
5Xg2q
Protein name
5Xg2q
Sequence length
266 AA
Molecular weight
29332,91730 Da
Isoelectric point
6,86168
Sequence
MALPTKNINGDIFSGLITDVDPNMMNADGNRTSIDRIVIHHNATTNDAVARSTWYVGGAAGTSAHYQVTPDKLWGCVGENMVAYHAGNYPMNQRSIGIEHLNETLEPGWTIAEATYQRSAALIADICQRHGIPIDREHIIKHGEVSSTACPGGIDIDRLVRMAKEIANGGGATPASAPSLKKYRGGHNLMFLMIFDEKVGSFNKYEIWLGDATRGVYFYINHPEELRFIKDSYKKNSGGLEIPEYHCSNGFPVHARLISGYKLKKI
Other Proteins in cluster: phalp2_11189
Total (incl. this protein): 31 Avg length: 264,2 Avg pI: 7,65
Protein ID Length (AA) pI
5Xg2q 266 6,86168
5HJTN 263 7,66311
5P6NH 266 5,98801
5TOAM 266 5,98551
5UKko 262 5,97295
5VTHm 250 8,22457
66RPq 257 7,65686
7UOML 266 5,92049
7Z7Na 258 7,63742
7jFmZ 258 8,46355
7jMaw 275 6,78535
7jxqW 257 7,65692
7wXYw 274 8,66089
7zVQH 258 8,17796
85ZtL 257 8,16449
8KUG9 289 7,01964
8LDTy 258 7,64015
8LkoO 258 7,62986
8LsE9 257 7,63742
8LsEB 257 8,16442
8LsFQ 257 7,07670
8LsFq 257 8,80143
8LsGh 258 8,47703
8LsH5 257 7,65697
8cmyC 192 8,31495
8giiH 290 8,31773
8hloX 257 8,47625
8nYVH 258 8,18363
8ontP 258 8,47032
8tRjm 253 8,88434
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_26061
7ewxf
 55 55,0% 267 5.832E-94
2 phalp2_23566
7vOVo
 2 40,1% 167 5.242E-43
3 phalp2_6357
7sHfT
 17 38,6% 176 1.725E-39
4 phalp2_28741
4J96O
 14 37,1% 226 1.611E-36
5 phalp2_4921
6qvcZ
 45 34,7% 184 1.588E-29
6 phalp2_13827
7cMO8
 12 27,5% 185 9.322E-19
7 phalp2_20231
8lqkG
 12 23,3% 236 7.746E-18
8 phalp2_30111
3bZzl
 4 24,8% 177 1.916E-17
9 phalp2_8918
3eAb5
 8 25,8% 197 1.580E-16
10 phalp2_558
HtN7
 28 25,3% 197 5.793E-15

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 5Xg2q (266 AA)
Member sequence: A0A4D6AWQ3 (401 AA)
1 266 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptococcus phage Javan274
[NCBI] 		2548089 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MK448895 [NCBI]
CDS location
range 31949 -> 33154
strand +
CDS
ATGGCGACAGTAGATGAGGTTATAGCAGATGTAGATTCTTTTGTTGGTCGATGCCTAGACATGGATGGCTACTGGGGCGGGCAATGCATGGATGTTATTGAACGTGTTGCTTCTCATTTTGGTAAACATCTAGGCGGTAATGCCATCGATGGTTTAACCAACGCTCAGCGTCTAGGGTGGCCAGTTATCATGAATGCTTGGGGAGTTAATCCTAAGCGCGGTGACGTTGGGGTAATGTCGGTACCTGGTCATGGATTTGGTCATATCTTCCTTGTGACTAAAGATAGTGATGGTGTGACGATCGAAACCCTAGAACAGAATATTGATGGTTATGCCGATAACAACAAGGATGGCATTAATGACCAGCTACAAGTTGGTGGTGTCGTTCGTCGACATAAACGTGACTTTACGGGTGTCATTGGTTGGTTCAGACCACCCTATGATACGATGAAAACAGGAGGCAAGACCATGTCTATCCCAACAAAAACAGTAAATGGTGATTTGTTTTCAGGGCTAGTAACAGATGTTGACCCCAATATCATGAACGGGGATTATAACCGTACTAAAATTGATCGCATTGTTATTCACCACAACGCAGGTACGTCTGATTCGGCAGCTCGTAGAACGTGGTATATTTCAACGGGGGTCGGTACCTCAGCGCATTATCAGGTAACCCCAGATAAGATTTGGGGGTGCGTTAGCGAAACTTCTGTAGCTTACCATGCTGGAGACTATGCCGTGAACCAACGGTCTATCGGTATCGAGCACCTTAATAATGCAGGATCTCCACATTGGACGATAGCAGAAGAGACTTACCACAATTCGGCTAAACTGATTGCGGACATCTGCCAGCGTTACAATATCCCAATTGACGATAAGCACATTATCCCACACAGAGTCGTTTCAGCGACTGCTTGTCCTGGTGGCATTGATATGGCTAAACTTATCCATATAGCCCAAGAAGTTGCAAGCGGAGCTATTTCCAAAACGGAAATAACTGTAAAACCCTCGGGCACTTTCCGAGTTAAGATTAGTGTATCTGATTTAAATATCAGAAAAGCCCCTAGCGTGTCTTCCAAGTCCCTTGGTTTCATCAAGCCGGGTGTTTATACCATCGTTGAGACAAAACAAGTAGAGGGCTATGAGTGGGGGCAATTAAAGAGTGGCATTGGTTGGATCGCTTTAAACTATACTAAGAAATTATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0046872 metal ion binding molecular function None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000ae929ac_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (5Xg2q) rather than this protein.
PDB ID
5Xg2q
Method AlphaFoldv2
Resolution 88.48
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50