Protein

Protein accession
A0A499SSY5 [UniProt]
Representative
7wAa9
Source
UniProt (cluster: phalp2_8239)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAKEKIGTWNGVPVYTDFLPIGTRRSGQRLVSGNPKFAVFHDTGNRDSTAQNNVDYYRNTYNIDWAYTASAHVFVDDKECIICIPVTEKAWHVLYDTPIDNNWYGDDANDIAFGLEACYFSDRDRTLKSLDNACRVMGALCASWDINPRNEMPGHQDIQFDKQDPGNILEAAGYGRSDMHIIDDLVVKYMNGDAPAPKVAKTVSQPKQGKPPKTVWAWHGIFTASDDNDDAIVVRRAFGMDAEEVDSGSWIYPGEYVAFDQVIKDVKNKMWWIRFKYQADGANKKDNFYMPIGKITDKDGKLLKEKALWGKLEVN
Physico‐chemical
properties
protein length:315 AA
molecular weight:35686,7 Da
isoelectric point:5,32
hydropathy:-0,56
Representative Protein Details
Accession
7wAa9
Protein name
7wAa9
Sequence length
532 AA
Molecular weight
60667,01110 Da
Isoelectric point
9,52412
Sequence
LTKKKIGVWNGVPVYTDFLPIGTRRTGQRLDSGEPKFAVFHDTGNKDTSAQANVNYFKNTYNIGWNQVASAHIFVDDVECIICIPVTEKAWHVLYNTTQDNFHFGDDANDIAFGIETSYFSSKTRSLKSLDNACRVMAALCNSWDINPKTHVPGHQNIQSDKQDPGNLLVACGYKRSDMKIIDDLIVKYMKGDAPVKKVAPKPEKVITANPKKAKSILSMSSKAYYKGTIKYDASLRRRKGSTLNDFSFGEEIGIVKKGIEVYIFQEIKDAQGNTWCRTYSNKNNGWVHKDTIKVTETFIKPKTKKKTVAKKESKVDKGIYTVKPGDYLSKIAKQHKVTVADLKKWNKLTSDKIFPKQLLKVVKPIETKPKTIKKQKAPVKQTTVQVANARTKKEMLDRLNWYVGKYIDFDNMWKYQCQDVAVDYVWYLTKGTYRLKGNARDNIDPKINQFPEGWKIYENTPEFLPQVGDIVVYTKDFADNQYGHVALVYANPTLTSMVVIEQNWNGQANLPCKLRTDYYKGVSHFIRPLVI
Other Proteins in cluster: phalp2_8239
Total (incl. this protein): 4 Avg length: 476,8 Avg pI: 8,44
Protein ID Length (AA) pI
7wAa9 532 9,52412
11Bke 531 9,43006
7vD0c 529 9,48834
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_23551
7qsYO
 131 38,5% 522 2.158E-105
2 phalp2_10314
1lVmq
 17 36,9% 511 6.331E-94
3 phalp2_10981
4SVbj
 2 25,4% 369 1.752E-13
4 phalp2_22268
7qxva
 11 25,1% 338 4.932E-08

Domains

Domains [InterPro]
Ami2
Unannotated
LysM
CHAP
Representative sequence (used for alignment): 7wAa9 (532 AA)
Member sequence: A0A499SSY5 (315 AA)
1 532 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01476, PF01510, PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage phiSP119-2
[NCBI] 		2491317 Triavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MK075005 [NCBI]
CDS location
range 39001 -> 39948
strand +
CDS
ATGGCTAAGGAAAAAATCGGGACATGGAATGGTGTGCCAGTATATACAGATTTCTTGCCAATCGGAACACGTCGTAGTGGTCAAAGGCTAGTGAGTGGCAATCCTAAGTTTGCGGTATTTCATGACACCGGAAATAGGGATAGTACTGCACAAAATAATGTCGATTATTATCGCAATACGTATAACATCGATTGGGCTTACACTGCATCAGCACACGTGTTTGTGGACGATAAAGAGTGTATCATCTGTATACCTGTAACAGAAAAAGCATGGCATGTACTGTATGATACGCCTATCGATAACAACTGGTACGGCGATGATGCAAATGACATTGCATTTGGGTTAGAGGCTTGTTATTTCAGCGACAGAGATAGAACGCTTAAATCATTAGACAATGCGTGTCGTGTTATGGGCGCGTTATGTGCGTCTTGGGATATTAATCCGCGTAATGAAATGCCTGGTCATCAAGATATTCAGTTTGATAAACAAGACCCAGGCAATATTTTGGAAGCTGCAGGGTATGGGCGCTCCGACATGCATATTATTGATGATTTAGTGGTTAAATATATGAATGGAGATGCACCAGCGCCGAAAGTGGCCAAAACGGTCAGTCAGCCGAAGCAAGGGAAACCACCTAAAACAGTGTGGGCGTGGCATGGTATTTTTACCGCTTCAGACGATAACGACGATGCGATTGTTGTACGTCGTGCATTTGGAATGGATGCCGAAGAAGTTGATAGCGGGTCATGGATTTACCCAGGCGAATATGTAGCGTTTGACCAAGTGATTAAAGATGTTAAAAATAAAATGTGGTGGATTCGATTTAAATATCAAGCAGATGGGGCAAATAAGAAAGATAATTTTTATATGCCTATTGGTAAAATTACTGACAAGGATGGTAAATTGTTGAAAGAAAAAGCCTTGTGGGGCAAGTTAGAAGTTAATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7wAa9) rather than this protein.
PDB ID
7wAa9
Method AlphaFoldv2
Resolution 84.98
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50