Protein

Protein accession
A0A494RGB7 [UniProt]
Representative
7rWDg
Source
UniProt (cluster: phalp2_16558)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MQIKTDYLVTSPSRWEALAPYSMNPTEITFHNTYNDASALNEVKNVYNNSTGTSFHTAVDDKEVQQVIPFNRNAWHAGDGASGRGNRHSIGVEICYSKSGGDRYRLAEKNAIDHISDLMIKFNIPIEKVKTHQERSGKYCPHRMLDEGRVKWFKDQLVSAVNFKKNGGGNVVVKPNTNILQSGGFGYIMATEVMDAMVQSGVTGKLVVLNDGTFYVETDRMQNETLWKLDNIFLARGWWFQYLG
Physico‐chemical
properties
protein length:244 AA
molecular weight:27552,7 Da
isoelectric point:7,77
hydropathy:-0,48
Representative Protein Details
Accession
7rWDg
Protein name
7rWDg
Sequence length
241 AA
Molecular weight
26652,06440 Da
Isoelectric point
9,28301
Sequence
MAYTIVNKFIPTSLYALKAPYAMDAQYITIHNTANDATAINEIAYMHRNPAATSYHVAVDDIHAVQAIPFSRNAWHAGDGASGQGNRKSIGIEICYSKSGGEKYKKAEANAIEYIAHVLHQYNWGIERVMWHRNWSGKNCPHRIFAEGRADEVKKRIADRLAELKKPSTEKPPVVVKPATPEKEVTPVSEPKNAPSSWAKRDVEAAVKLGISDGSRLQEPVTREEAIVLAMRAAGLAPRIK
Other Proteins in cluster: phalp2_16558
Total (incl. this protein): 20 Avg length: 248,4 Avg pI: 8,96
Protein ID Length (AA) pI
7rWDg 241 9,28301
11BwF 291 9,62224
1mvDa 249 9,12158
35mI1 224 9,23782
3MRZI 230 9,15014
76rku 230 9,21300
7epKf 310 9,24375
7hBFm 229 9,29906
7lJFd 230 9,39525
7nRsu 265 9,37559
7oizO 226 9,26741
7z7bH 231 9,33787
8tZgi 237 8,82277
D54K 242 8,59907
D5CT 238 8,37059
A0A2P1JUJ2 233 8,72955
A0A7D5JGS8 290 8,81304
A0AAE8YRS0 278 8,83521
A0AAE9ZMW6 250 7,66186
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_13879
7z7dc
 255 53,7% 216 6.095E-80
2 phalp2_24718
6w5Af
 15 43,4% 205 8.686E-71
3 phalp2_5182
3wjGA
 273 55,4% 164 2.016E-69
4 phalp2_37301
7Ya12
 3 52,0% 169 1.095E-60
5 phalp2_25038
13C0m
 57 48,6% 146 7.919E-52
6 phalp2_13869
7vyfo
 1 49,6% 149 9.726E-51
7 phalp2_39055
7ordh
 68 38,7% 217 2.197E-46
8 phalp2_9318
7vPuw
 15 33,2% 253 1.650E-38
9 phalp2_22227
73VY4
 57 41,1% 180 2.254E-38
10 phalp2_33479
7swgL
 97 44,2% 174 3.737E-37

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 7rWDg (241 AA)
Member sequence: A0A494RGB7 (244 AA)
1 241 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage vB_BthS_BMBphi
[NCBI] 		2498843 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MH458951 [NCBI]
CDS location
range 23113 -> 23847
strand -
CDS
ATGCAAATCAAAACAGATTATCTTGTAACGTCTCCCTCTCGGTGGGAGGCGCTTGCACCTTACAGCATGAACCCTACCGAGATTACCTTCCACAATACCTACAATGATGCTTCAGCCTTAAATGAGGTCAAGAATGTGTATAACAACTCTACAGGCACAAGCTTCCATACAGCTGTAGACGATAAAGAAGTCCAACAAGTCATCCCCTTTAACCGCAATGCATGGCACGCTGGAGATGGAGCTTCAGGGAGAGGCAATCGTCACAGTATCGGCGTAGAGATTTGCTACTCTAAGAGTGGTGGAGATCGTTATCGTTTAGCAGAGAAAAACGCTATTGACCATATCAGTGACTTGATGATTAAGTTCAATATCCCTATCGAGAAGGTCAAAACCCATCAAGAGCGCAGCGGTAAGTACTGCCCTCATAGAATGCTTGACGAGGGTCGAGTGAAATGGTTCAAGGATCAACTTGTAAGTGCTGTAAACTTTAAGAAAAACGGAGGAGGAAACGTAGTGGTAAAACCTAATACGAACATATTACAGTCTGGTGGATTCGGATATATAATGGCTACAGAGGTAATGGACGCAATGGTACAATCAGGAGTAACTGGAAAGTTAGTGGTCTTAAATGACGGGACATTCTATGTAGAAACTGATAGAATGCAAAATGAAACTCTCTGGAAGTTAGACAATATCTTCTTAGCTCGTGGATGGTGGTTCCAATATTTAGGGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7rWDg) rather than this protein.
PDB ID
7rWDg
Method AlphaFoldv2
Resolution 89.15
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50