Protein

Protein accession
A0A3G8FR37 [UniProt]
Representative
7wVIM
Source
UniProt (cluster: phalp2_17757)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MPAYTQDGIAIGIIAEGRRSRSGEGQLDHPVISEKGIVIALAVALVETNLKMYANRSDPESLNFPHDAVGSDANSVGVFQQRAPWWGTVADRMDVARSAAMFYNSLYRQRVGGADYNTDRVSPGTWGQMVQQSAFPDRYDKRMAEARQIYDRLKDRVVGGAPTPPPITAPDPNWRGDPVWLKEVLEAAGLVCHVYDGAYNRGHGDFGEIWGVVAHHTGSFGETPKGIAQHPSLGLASQLYLGRNGEYTLCGVGIAWHAGQGSYPGLPTNDANRLTIGIEAANDGGGSPPGKRDAWSDVQYNAYVRGVAAILRKLGRDSSRVIGHKEWAGTAQGKWDPGGIDMNTFRADVARVMGELGTSTDPVLELLAMPTNQEKLDFIYNEESKKFASRSIYRTPGEGLIDTRAGFVLNVDAMAHQELVDRLAIQYHDSDAIGRIARVAAGQGADPNDTWAKEHALTVLQKIPEEVLKAWQEKNR
Physico‐chemical
properties
protein length:476 AA
molecular weight:51860,4 Da
isoelectric point:5,88
hydropathy:-0,43
Representative Protein Details
Accession
7wVIM
Protein name
7wVIM
Sequence length
464 AA
Molecular weight
50839,29520 Da
Isoelectric point
5,92037
Sequence
MVTKDQVAQLIVGEAKSRNHTRDECLAEMSALYQESQWDETVWDPTHTTYGVAQQDGSYPNRFQGAAAQIKAFFDKLDVKRASPGRGDIWLNICWLQQAPNWLSAQYWYDNGRRAYLAEIKSRVATVTPYLERYWPATGTPTIPVDTAPPVGDPVWLPDVLRAAGLRCDIYPGAMDRGHGDFGRIWGVVCHHTGSFGETPRGIAEHPELGLASQLYLSRDGVFTLCGVGIAWHAGAGSWPGIPTNNANQVTIGIEAANDGGGTPGKPHRTGWSDAQYNAYVTGVAAILNKLGQPADHAIGHKEWAGAAQGKWDPGAIDMNIFRADVAREQARLKGAASTDPIEELLMMELESTSPFATPGEPKIPAEVMLRNADAAGHRALVLDDAEDGDQDAIFRFVRAARGHDSRYTDAPSVAKWRRRLNRFIRRTAVDAASGDTTAVGILRHMQDKYPDVLQDFINTRKGN
Other Proteins in cluster: phalp2_17757
Total (incl. this protein): 110 Avg length: 435,2 Avg pI: 6,17
Protein ID Length (AA) pI
7wVIM 464 5,92037
3lLVF 407 5,20409
6Ugxv 424 4,52236
6Vwoo 424 4,48627
7AK6J 414 6,04019
7aY3y 406 5,95709
7iKFq 416 5,65562
7r406 392 5,71450
7ubx8 386 5,04221
7udcz 390 5,05955
8dQhs 408 5,38933
A0A4D6E276 354 8,76559
A0A455LM17 510 5,77498
A0A386KB78 528 5,68301
G8IAJ2 476 5,87729
G8I9G3 577 5,33737
A0A3Q9R8M1 366 6,76346
A0A1B3AZZ3 366 6,59755
A0A162E1U7 370 6,63813
A0A2Z4Q823 381 6,11942
A0A2P1A0T5 366 6,95672
A0A159B6C4 366 6,56680
A0A2K9VH40 366 6,97906
A0A5Q2WFK5 551 5,40189
A0A2Z4Q8W9 381 6,14489
A0A346FCL4 344 6,75704
A0A346N934 476 5,87735
A0A345MB69 381 6,01427
A0A0A0YSC3 476 5,87729
A0A0K2FMG8 476 5,87729
A0A1I9S8J3 476 5,87729
A0A1I9S980 476 5,87729
A0A1J0MA43 476 5,97636
A0A2D1G768 476 5,87729
A0A2D1G805 476 5,97636
A0A2R4ARN9 476 5,97636
A0A2U8UNQ7 366 6,97906
A0A346FDZ5 476 5,87729
A0A386KC63 476 5,87729
A0A3G3BZH6 476 5,87729
A0A3G8FL16 476 5,87729
A0A3G8FMS9 476 5,87729
A0A3G8FNF9 476 5,87729
A0A3G8FPG7 476 5,87729
A0A3G8FTQ8 476 5,87729
A0A3S9UAG8 391 8,64548
A0A411AZJ8 476 5,87729
A0A411BSJ2 476 5,87729
A0A411BU73 476 5,87729
A0A411BXV9 476 5,97636
A0A411C1Y0 476 5,87729
A0A411C322 476 5,97636
A0A411C3R6 476 5,87729
A0A411C646 476 5,87729
A0A411C6N5 476 5,97636
A0A411C7G3 476 5,87729
A0A411C898 476 5,87729
A0A411CC32 476 5,87729
A0A411CCV4 476 5,97636
A0A411CFG6 476 5,97636
A0A411CH49 476 5,97636
A0A411CII1 476 5,87729
A0A411CJS4 476 5,69103
A0A411CKM4 476 5,97636
A0A411CMX7 476 5,87729
A0A411CNX9 476 5,87729
A0A411CUP2 366 6,76358
A0A411CYB2 476 5,97636
A0A4Y6EGW4 366 6,76312
A0A514DIC5 366 6,76324
A0A514U119 354 8,27814
A0A516KPP4 366 7,83886
A0A5P8DBM9 366 6,76312
A0A5P8DEZ3 366 6,59704
A0A649V6J7 366 6,95592
A0A649VJ89 336 7,29110
A0A6B9LCN8 366 6,98002
A0A6N0A3K1 476 5,87729
A0A7D5FN11 366 6,73613
A0A7G8LDM1 368 6,73863
A0A7L7SIL8 366 6,76312
A0A7L7SQC1 381 6,01319
A0A7L7SRP0 476 5,87729
A0A7M1CRY1 476 5,87729
A0A7T0Q2Y4 476 5,97636
A0A7T3N175 366 6,95683
B5LLI8 476 5,87729
B5LME5 476 5,87729
C9DB30 476 5,87729
G1BKV8 476 5,87729
G1FIV9 476 5,87729
G1JVT3 476 5,97636
G1JXV8 476 5,87729
G3M575 476 5,87729
G8IDH4 476 6,08373
G8IE58 476 5,97636
R4TQV7 476 5,87729
A0A8A5LJA3 356 7,84795
A0A8A5LKQ6 344 6,75704
A0A8F2IF64 369 5,87956
A0A976UAA1 476 5,97818
A0A977KNF2 366 6,73732
A0A977PS15 366 6,95672
A0A9E7J4D0 405 6,27323
A0A9E7NKJ8 367 7,24944
A0A9X9K447 366 6,76420
A0AA51BPA3 476 5,97818
A0AA96KAI8 366 6,76312
A0AA96NKG5 476 5,87729
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_40628
7RbAk
 146 46,5% 329 1.367E-98

Domains

Domains [InterPro]
Unannotated
Ami2
Unannotated
Representative sequence (used for alignment): 7wVIM (464 AA)
Member sequence: A0A3G8FR37 (476 AA)
1 464 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Mycobacterium phage Jessibeth14
[NCBI] 		2488969 Bixzunavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MK112541 [NCBI]
CDS location
range 143730 -> 145160
strand +
CDS
GTGCCCGCATACACACAGGACGGCATCGCCATCGGCATCATCGCCGAGGGGCGACGTTCGCGGTCTGGAGAAGGCCAGCTTGATCACCCGGTGATCAGCGAGAAGGGCATTGTCATCGCGCTCGCCGTCGCCCTGGTCGAGACCAACCTCAAGATGTACGCCAATCGGTCGGACCCGGAGAGCCTCAATTTCCCGCACGACGCGGTCGGCAGCGACGCCAACAGCGTCGGCGTCTTCCAGCAGCGCGCTCCCTGGTGGGGCACGGTCGCCGATCGCATGGACGTCGCGCGCAGCGCGGCGATGTTCTACAACAGCCTGTATCGCCAGCGCGTCGGCGGCGCCGACTACAACACCGATCGGGTCTCTCCCGGTACCTGGGGCCAGATGGTCCAGCAGTCCGCCTTTCCGGATCGCTACGACAAACGTATGGCCGAGGCCCGGCAGATCTATGACCGGCTCAAGGACCGAGTGGTCGGCGGTGCTCCTACTCCGCCGCCGATCACCGCCCCGGACCCGAACTGGCGCGGCGACCCCGTCTGGCTCAAGGAGGTCCTCGAAGCCGCAGGCCTGGTTTGCCACGTCTACGACGGCGCCTACAACCGGGGCCACGGCGACTTCGGCGAGATCTGGGGCGTGGTGGCTCACCACACCGGCAGCTTCGGCGAGACGCCCAAGGGCATCGCTCAGCACCCCAGTCTGGGCCTGGCCAGCCAGCTCTATCTGGGCCGTAACGGCGAGTACACCCTGTGTGGGGTCGGTATCGCCTGGCACGCCGGGCAGGGCAGCTATCCCGGCCTGCCGACCAACGACGCCAACCGGCTCACCATCGGCATCGAAGCGGCCAATGACGGGGGCGGCTCTCCTCCTGGCAAACGCGATGCCTGGAGCGATGTGCAGTACAACGCCTATGTCCGTGGCGTTGCCGCCATCTTGCGCAAACTCGGTCGGGATTCCAGCCGGGTGATCGGCCATAAGGAGTGGGCCGGTACCGCGCAGGGCAAGTGGGACCCCGGCGGCATCGACATGAACACCTTCCGCGCCGACGTGGCCCGTGTGATGGGCGAACTTGGCACCTCGACCGACCCAGTATTGGAGCTACTCGCCATGCCTACCAATCAGGAGAAGCTGGACTTCATCTACAACGAGGAGTCGAAGAAGTTCGCCTCGCGTTCGATCTACCGCACTCCGGGTGAGGGCCTGATCGACACTCGGGCCGGATTCGTCCTCAACGTCGACGCCATGGCTCATCAGGAGCTCGTCGACCGGCTGGCCATCCAGTACCACGATAGCGACGCCATCGGGCGCATCGCGCGGGTGGCCGCTGGCCAGGGCGCCGACCCCAACGACACCTGGGCAAAGGAGCACGCGCTCACGGTGCTGCAGAAGATCCCCGAAGAGGTCCTCAAGGCCTGGCAGGAGAAGAACCGATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0001b621f9_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7wVIM) rather than this protein.
PDB ID
7wVIM
Method AlphaFoldv2
Resolution 82.14
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50