Protein

Protein accession
A0A3G3BWD6 [UniProt]
Representative
7loRH
Source
UniProt (cluster: phalp2_24820)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTYQIKKNKLKWRNSGWVKLNPNAVTGIALHHMAHPTADLETVHDWHLARWNNEPGAGYNYFVTLEGEIIEARGDHRGAHTGGHNSSLLGIGFQGNYHPIKGVAHRTSMPDKQYNAGVWLIKELQKKYPQARRVHGHKHWTATACPGDFFPLVEMLGGKYRGQAQPVQQPTAPAAAAGTYTVKDGDTLGRIAAANGTTTEELAKLNGLSNPDLIRVGQEIKLPVTTYTVKAGDTLGRIAQQHNTTVEALAQLNGISNPDRISVGQVLKVTGTTATAPKPTPQPAPKPSTNVPAATLRPGTRSAAVGQLQRALNTHSPRFNPGAIDNSYGPKTQDAVKRYQMYYGVRPFDGIYGPLTAAKLRETSK
Physico‐chemical
properties
protein length:365 AA
molecular weight:39618,3 Da
isoelectric point:9,83
hydropathy:-0,56
Representative Protein Details
Accession
7loRH
Protein name
7loRH
Sequence length
328 AA
Molecular weight
34939,24810 Da
Isoelectric point
10,04638
Sequence
VATAVDFYTRVANRLRSWGFNVEEVPGWQSRDAEPKTAFDPTQLFVEHHDASAATSVDGSHAYVVREKLSQFTINRSGLIRLCAAGITWHAGKGGPLHGVPLNNANPRSTAVEVANNGLGEPYTDACTASIVALEAAWCMEAGRGVERVVGHREWAPTRKIDPRIDMNWRRAAVAAFVASKSAAPAPASAAGSYTVRAGDTLGGIAKAHGTTWQVLAQLNGLGNPNVIRVGQVLRLPGAAAPTPAPAPAPAPAALSPDPKANPIAFQRWYNAYPFRPALLPVIRPLANNWGPQSDRALRKVQARYGLAVDGIPGPKTRALLHRLGYRG
Other Proteins in cluster: phalp2_24820
Total (incl. this protein): 2 Avg length: 346,5 Avg pI: 9,94
Protein ID Length (AA) pI
7loRH 328 10,04638
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_12291
7ggQn
 288 32,1% 352 7.286E-32
2 phalp2_3982
7wtdu
 333 32,4% 256 1.134E-30
3 phalp2_40432
4fxAl
 3 29,1% 326 5.569E-17
4 phalp2_10221
T1NY
 7 31,1% 247 1.499E-13
5 phalp2_36904
7xR8d
 63 25,0% 291 8.259E-08

Domains

Domains [InterPro]
Ami2
LysM
PG_1
Representative sequence (used for alignment): 7loRH (328 AA)
Member sequence: A0A3G3BWD6 (365 AA)
1 328 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01476, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage vB_BboS-125
[NCBI] 		2419618 Elmenteitavirus > Elmenteitavirus ev125
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MH884509 [NCBI]
CDS location
range 8087 -> 9184
strand +
CDS
ATGACTTACCAAATTAAGAAGAATAAATTAAAATGGCGTAACAGCGGCTGGGTAAAACTGAACCCGAACGCCGTAACAGGGATAGCCTTACACCATATGGCACACCCTACCGCGGACCTGGAAACCGTACACGACTGGCACCTAGCGCGCTGGAATAACGAACCAGGCGCAGGCTATAACTACTTCGTAACGCTAGAAGGGGAAATAATCGAAGCCCGCGGGGACCACAGGGGCGCCCATACTGGCGGCCATAACAGCAGCCTTTTAGGTATCGGCTTCCAGGGGAACTACCACCCGATTAAGGGCGTAGCGCACCGTACAAGTATGCCAGATAAGCAGTATAACGCTGGCGTATGGCTTATTAAGGAACTACAGAAGAAGTACCCGCAGGCCCGCCGCGTACATGGACATAAACACTGGACAGCCACAGCCTGCCCTGGTGACTTCTTCCCGCTGGTAGAAATGCTAGGCGGTAAGTATAGAGGACAGGCCCAGCCCGTCCAACAACCGACGGCGCCAGCTGCAGCTGCTGGAACTTATACCGTAAAAGATGGGGACACGTTAGGACGTATAGCAGCTGCTAACGGAACGACGACCGAAGAACTGGCTAAACTTAACGGCTTAAGTAATCCAGACCTAATAAGAGTAGGGCAGGAAATTAAACTACCCGTAACAACGTACACAGTAAAAGCGGGGGACACGTTAGGACGTATAGCGCAGCAGCATAATACAACAGTAGAAGCCCTGGCACAGCTTAACGGTATCAGTAACCCCGACCGTATTAGCGTAGGCCAGGTACTAAAGGTAACAGGAACGACAGCAACGGCACCAAAGCCGACGCCGCAGCCAGCGCCTAAACCTTCTACGAACGTACCAGCTGCAACCTTACGACCTGGGACGAGAAGCGCAGCCGTAGGCCAGCTGCAGCGCGCACTTAATACACATAGCCCGCGCTTTAACCCTGGGGCTATCGACAACAGCTACGGCCCTAAAACACAGGACGCCGTAAAAAGGTACCAGATGTACTACGGGGTAAGACCCTTCGACGGTATTTACGGACCTTTAACAGCTGCAAAACTACGGGAAACTTCGAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0008932 lytic endotransglycosylase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. N/A UniProt

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7loRH) rather than this protein.
PDB ID
7loRH
Method AlphaFoldv2
Resolution 83.37
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50