Protein

Protein accession
A0A3G3BVI7 [UniProt]
Representative
29vRH
Source
UniProt (cluster: phalp2_6744)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAWKNQYITPNKFSRPQHKLIGVRKIVVHYTANHGAGADNHYRYFNSLKDRYASAHIFVDRKEALCIIPLDEVAYHANDGGKSLISQLVATTSYYKNGNANLTSIGVEMCMESNGTIHADTIKRTEDVVAELCKRFKLNPLNDVVRHYDVTRKNCPAPWVSNSSLFTGFKTRVNNKLNPPKTSQPSQPTTGFVSVGELGVATVKVHTLNVRSQSNINGRIVREVKNGNKLPVYEIKNGWYRIGVNEWISNSGNAYANYSAHKKGSRIGTAVVNASVLNVRTTSNANGSIVGQVNKGDKLEVFQVRDNWLRIGVNAWVSNVGNSYTTYTPNLAPKPTATKPKVDESIFYRVVVGSFKDKKQAEDRVKVLKSKKVDSFVLPFVDGKTTFYRVIAGSFNDRANAEQTITNLKKLGFESFIAVYKK
Physico‐chemical
properties
protein length:422 AA
molecular weight:47044,9 Da
isoelectric point:9,85
hydropathy:-0,44
Representative Protein Details
Accession
29vRH
Protein name
29vRH
Sequence length
523 AA
Molecular weight
57420,83740 Da
Isoelectric point
9,58002
Sequence
MAIWKDKFIKPNKYSRPQTKLTAVRKIVMHWTANFGATANGHYNYFNTLSGRYASAHLFVDKIEALCIVPLNEITYHANDGTYKGVPELKPNANFLSIGIELCVEKDGSFHPDTLKRAMQVAFELCEIYKLDPLTDIVRHYDVTHKSCPSPWVQNASLFTGFKNDVKKLATPKPVVAVDPHKNHSAIAGKSVVQYDKMASFVKSINPKAVDIDAIAKAFVTVGEKYGIRGDVAFCQAIIETSWFKFDGGTAVTPDQHNYCGMGVTQKGLKGSSFATVSEGVTGHIQHLFGYASKAVLPTGEKLLDPRFHLVTRGIAPHWEDLSNRWAMNADYGKHILALYKQLGEFKYVAPKPVVAVVAKPTPAPAPVAKPVVAPKPVAKPKSHTVKQGETFYSISKKYGMTSDELQKLNPRVKSGELQIGDVIHLISIPVSVAPKPKPKPVTKPTIKKLSFHRTLAKGSKGNDVKALQEALNKLNFKVGIVDGSFGSKTEDAVMRFQKVYLSGEVDGLAGKNTISKINNLLK
Other Proteins in cluster: phalp2_6744
Total (incl. this protein): 5 Avg length: 574,2 Avg pI: 7,34
Protein ID Length (AA) pI
29vRH 523 9,58002
4TxlI 664 5,34027
5yXXA 516 5,63578
7JoWO 746 6,26942
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3828
6v3ib
 489 37,6% 356 5.605E-67
2 phalp2_10091
5D9X
 97 31,9% 369 1.114E-49
3 phalp2_1125
1lyG
 9 29,2% 359 3.889E-28

Domains

Domains [InterPro]
Ami2
GLUCO
LysM
PG_1
Representative sequence (used for alignment): 29vRH (523 AA)
Member sequence: A0A3G3BVI7 (422 AA)
1 523 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01476, PF01510, PF01832

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage vB_BcoS-136
[NCBI] 		2419619 Kenyattavirus > Kenyattavirus kv136
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MH884508 [NCBI]
CDS location
range 94498 -> 95766
strand +
CDS
ATGGCATGGAAAAATCAATATATTACACCAAACAAATTCTCTAGACCACAGCATAAGTTAATAGGTGTTAGAAAAATTGTTGTTCACTATACGGCTAATCATGGTGCAGGCGCAGATAATCACTATAGATATTTTAACAGCTTAAAAGATAGATACGCTTCTGCTCATATTTTCGTAGACAGAAAAGAAGCTTTATGTATTATTCCTTTAGATGAAGTAGCTTATCATGCGAATGATGGTGGTAAAAGTTTAATTTCACAATTAGTAGCGACAACATCTTACTATAAGAATGGAAATGCAAACTTGACTTCTATTGGTGTTGAAATGTGTATGGAGTCAAACGGCACAATTCACGCTGATACAATTAAGCGTACAGAAGATGTTGTAGCTGAGTTATGTAAACGCTTTAAGCTTAATCCGCTAAACGACGTTGTTCGTCATTATGATGTTACTAGAAAAAATTGTCCTGCACCTTGGGTGTCAAACAGTTCATTATTTACTGGATTTAAAACTCGTGTAAATAATAAATTAAATCCACCAAAAACTTCACAGCCTTCACAACCGACAACAGGATTTGTTTCTGTTGGAGAATTGGGAGTTGCAACTGTTAAGGTTCATACACTAAATGTTCGAAGCCAATCAAATATAAATGGTAGAATTGTTAGAGAAGTTAAAAATGGTAATAAATTGCCTGTATATGAGATTAAAAATGGTTGGTATAGAATAGGTGTTAATGAGTGGATTTCAAATTCTGGAAACGCTTACGCAAATTACTCTGCTCATAAAAAAGGTAGCAGAATCGGAACGGCAGTTGTAAATGCTAGTGTTCTAAATGTAAGAACAACTTCTAACGCTAACGGAAGTATTGTAGGTCAAGTTAACAAAGGAGACAAACTAGAAGTCTTCCAAGTTAGAGATAATTGGTTAAGAATTGGTGTTAATGCTTGGGTTTCAAACGTTGGAAATAGCTATACTACATATACTCCAAATCTTGCACCAAAACCTACTGCTACAAAACCAAAAGTAGATGAAAGCATATTTTATCGTGTTGTAGTTGGTTCTTTCAAAGATAAAAAGCAAGCAGAAGATAGAGTGAAAGTATTAAAATCTAAAAAAGTTGACTCGTTTGTTCTTCCATTTGTAGATGGAAAAACAACATTTTATAGAGTTATTGCAGGCTCATTTAATGATAGAGCTAATGCAGAACAAACAATTACTAATCTTAAAAAATTAGGCTTTGAATCATTTATCGCTGTCTATAAAAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0042834 peptidoglycan binding molecular function None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (29vRH) rather than this protein.
PDB ID
29vRH
Method AlphaFoldv2
Resolution 84.75
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50