Protein

Protein accession
A0A2U8UNJ8 [UniProt]
Representative
7o7fl
Source
UniProt (cluster: phalp2_20262)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MARFSGATWKPISVNHTKNGQESVRGVVIHIMDGTLAGTDSWFRNSKAQASSHFGTGKSGQLYQWVDTKDRAWAQANGNSDWLSVENEGRGGDSLTNAQLDRCADVLRWAHEVYDVPLKVASGPGDRGLGWHAMGGSSWGSHPSCPGSKIIAQLPEIVRRAGGKPSGGSSGGGGVARYKVTINGLEYGYGAEGSHVTAVGKALVSKGFGKHYQSGPGPNWSDADTKNYQDFQASLGYSGSDADGVPGESSLKKLLGKLPSASKPKPAPVSHEPFPGTAYFRKNPKSSLVTRMGKRLVAEGCSAYSSGPGPQWTEADRKSYRKWQLKLGYRGSDADGYPGKSSWDRLKVPKA
Physico‐chemical
properties
protein length:351 AA
molecular weight:37360,1 Da
isoelectric point:9,61
hydropathy:-0,67
Representative Protein Details
Accession
7o7fl
Protein name
7o7fl
Sequence length
376 AA
Molecular weight
38496,62190 Da
Isoelectric point
9,73790
Sequence
MARMTGATWRPIPVNYTGGGQTSVRGVVVHIMAGTLAGTDSWFRNSAAQASSHFGTGRSGALYQWVDTKDRAWAQADGNRSWLSVENEGKGGDALTDAQLDRNAQVLAWAHEVYGVPLQVANGPSGSGLGWHGMGGSAWGGHTSCPGSKVVAQLPEIVKRAKAIVGGSGTAPQGGSSSTPVTSSVSRATVTINGLTYGYGAKGDHVTAVGKALVKNGCSAYAEGPGPTWSDADTRSYQKWQTKCGYSGADADGVPGEVSLKKLLGKLPSKPAAKPSKPKVSLKNVIAAARRDPGLRQGGTTHAADVKVVEAALKAEKLLSAAYASDGSFGSITVAAYAKWQRSKAGGSYTGSAADGIPGSDSLKRLGARHGFTVVA
Other Proteins in cluster: phalp2_20262
Total (incl. this protein): 57 Avg length: 291,8 Avg pI: 9,62
Protein ID Length (AA) pI
7o7fl 376 9,73790
1jn4c 265 9,66621
4E5HT 354 9,66260
5inXv 349 9,62734
6CCQQ 270 9,78535
6D3JN 259 8,81007
6DC9l 254 9,85382
6DCuM 321 9,83467
6DWYa 321 9,87399
6De5i 269 9,67169
6Dg5V 259 8,96248
6DkH5 269 9,47152
6Dlio 269 9,47152
6DmeS 321 9,76582
6Dwuj 269 9,72082
6DxYt 269 9,53869
6ECiz 272 9,65093
6EKKR 269 9,67169
6ER1N 269 9,49324
6Eslh 320 9,90268
6EvGi 269 9,59742
6FoOV 269 9,72043
6Fpho 270 9,66911
6GGf0 269 9,67169
6H4G7 241 9,64977
6J6Wa 241 9,67240
6J7UK 288 9,88418
6J96j 256 9,78677
6KILU 241 9,61986
6KJAo 247 9,67266
6KPjE 269 9,47152
6Kqbr 321 9,78296
6OeOw 267 9,66873
6TWed 269 9,67169
6Tc3Q 358 9,53386
6Timy 307 9,77084
72ywv 278 5,88684
7AePG 361 9,25129
7AtRa 254 9,85581
7eRbl 306 9,70393
7o50r 376 9,86536
7o6Rf 306 10,01518
7oi0j 361 9,88553
7ouCj 256 9,88740
7ubTt 313 9,82667
7ucBO 256 9,99893
7ucCQ 353 9,68394
7uk7X 260 10,07752
7zaWU 270 9,72043
7zeNn 258 10,24643
7zf8f 256 9,70683
7zfPq 261 9,91422
7zhbu 270 10,28240
8Mr6i 360 9,22312
A0A386KNX3 361 9,25129
A0A1J0MCQ0 360 9,22312
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_2602
6IbUl
 53 36,4% 269 3.783E-43
2 phalp2_14741
6KOub
 4 38,1% 254 4.595E-35
3 phalp2_16498
6Tg4q
 5 26,3% 364 1.338E-30
4 phalp2_21629
2MzZy
 22 27,0% 414 5.938E-27
5 phalp2_23479
6PqkT
 19 26,3% 398 1.079E-26
6 phalp2_39542
5FB2E
 5 27,5% 294 1.454E-26
7 phalp2_16495
6RQrs
 8 30,3% 339 5.566E-24
8 phalp2_29674
8k36v
 96 22,5% 275 7.382E-13
9 phalp2_7198
3ejcO
 5 23,2% 374 7.227E-12
10 phalp2_5505
3kRYJ
 67 23,4% 277 1.524E-08

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Representative sequence (used for alignment): 7o7fl (376 AA)
Member sequence: A0A2U8UNJ8 (351 AA)
1 376 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptomyces phage Ibantik
[NCBI] 		2182397 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MH155870 [NCBI]
CDS location
range 47789 -> 48844
strand +
CDS
ATGGCTAGATTCAGTGGAGCAACTTGGAAGCCTATATCTGTCAATCACACCAAGAATGGACAGGAATCTGTCCGCGGAGTCGTCATCCACATCATGGACGGCACTCTGGCCGGCACGGACTCTTGGTTCCGTAACTCGAAGGCTCAGGCTTCCAGCCACTTCGGTACTGGGAAGTCTGGGCAGCTCTATCAGTGGGTTGACACCAAGGACCGTGCTTGGGCTCAGGCCAACGGCAACTCCGACTGGCTGTCTGTCGAGAACGAGGGACGCGGTGGTGACTCACTGACTAACGCTCAGCTTGACCGGTGTGCAGATGTCCTTCGTTGGGCTCATGAGGTCTACGACGTGCCCCTCAAGGTTGCCTCGGGCCCTGGCGACAGAGGCCTTGGCTGGCACGCTATGGGTGGTTCGTCCTGGGGGAGTCACCCTTCCTGCCCGGGTTCAAAGATCATTGCTCAGCTCCCTGAGATCGTGCGCCGTGCCGGCGGAAAGCCGTCAGGCGGTTCCTCGGGCGGTGGTGGTGTTGCCCGTTACAAGGTGACGATCAACGGCCTGGAGTACGGCTACGGAGCCGAAGGCTCCCACGTCACTGCTGTTGGCAAGGCCCTGGTCAGCAAGGGCTTTGGCAAGCACTACCAGTCAGGTCCTGGTCCCAACTGGAGCGATGCGGACACGAAGAACTACCAGGACTTCCAGGCGTCTCTTGGTTACTCGGGCAGCGACGCTGATGGCGTCCCTGGTGAATCGTCCCTGAAGAAGCTCCTGGGCAAGCTGCCTTCGGCCTCGAAGCCGAAGCCTGCTCCTGTCTCTCACGAGCCCTTCCCGGGTACTGCGTACTTCAGGAAGAACCCCAAGTCCTCCCTGGTGACTCGTATGGGGAAGCGCCTGGTTGCCGAAGGGTGTTCCGCATACTCCTCGGGCCCGGGTCCTCAGTGGACCGAGGCTGACCGGAAGAGCTACCGGAAGTGGCAGCTCAAGCTTGGCTACCGCGGCAGTGATGCTGACGGCTACCCCGGTAAGTCGAGTTGGGACCGGTTGAAGGTGCCGAAGGCTTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7o7fl) rather than this protein.
PDB ID
7o7fl
Method AlphaFoldv2
Resolution 90.30
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50