Protein

Protein accession
A0A2K9VH44 [UniProt]
Representative
netU
Source
UniProt (cluster: phalp2_2869)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAKLTPDATRTEHGLVINEKIIPWGAVWPKDSGAYKKGTQYKADRLLSGGTGKVKGVTIHNTADLKNVEEDAEQYTRATWPNGNMNDARVHYYVDDINAWQNLREDEVGWHAGDGRKATGGNETTLSIEIIMDGTGSKEDRKAEENGVLLAALLLKKHGLSVNELYTHNHWMGHPDSIVQGARKNCPLYILPHWAQFKQKVAAKLAELNGGATTTETGKTEIMGKAKASAQQMALFARSKNAEPQLPACSLEQLAQFFLEEGEAEGVRGDVAFAQSLHETGFFKYGGIVLPTQNNYAGIGALNGNAKGQAATFPDPRTGVRAQIQHLKAYASKAALVNGCVDPRFSLVTRGSAQYVEWLGASDNPNGKGWAVPGKGYGSKIVALLGQIMAFEVPQPSTPSEPEEQEPEFPAYQLEGLETLVEADVINSPEFWRQKFGEQVTVGELFGILGKLFTKASE
Physico‐chemical
properties
protein length:458 AA
molecular weight:49794,4 Da
isoelectric point:5,85
hydropathy:-0,45
Representative Protein Details
Accession
netU
Protein name
netU
Sequence length
452 AA
Molecular weight
48735,52740 Da
Isoelectric point
6,66724
Sequence
MTWMANPFFTPTRKQVTAMSKVKICLDAGHIGSTYNQSPVVKTYYESTMVWKLHLKLKTALEQRGFEVVTTRAAIDTPMDVYARGAAAKGCDVFLSLHSNACDTESVDYPVIYRAYDNQNNTDTLARALALSIGQLMGTRQQGRTATRRNNAGGEYYGVLRGARAAGVPVYLLVEHSFHTNTAAAKWLSVDENLDRLAEAEADLLAGYFNVQTVPEIRTSIMGTAQATAQQMALFCRSRNPAPQITGCSLEALAQMYLIEGATEGVRGDVAFAQSLHETGFFRFGGIVQPGQNNFAGIGALNGNTSGQAATFPNALTGVRAQIQHLKAYASTEPLKNPCVDPRFGLVTRGSAPYVEWLGAEDNPGGAGWAYPGKGYGASVLTLLSQLIAQTVPQPKPPQSPAVPDWQRSGLQTLTEAGVINSPDYWEARLSQNVTIGELFGILGNLFTEVPG
Other Proteins in cluster: phalp2_2869
Total (incl. this protein): 143 Avg length: 432,6 Avg pI: 6,69
Protein ID Length (AA) pI
netU 452 6,66724
138UG 559 6,30256
13DtB 406 5,72087
13rAw 433 5,62941
1FXSU 433 5,89838
1FY6s 430 8,54910
1HY3t 383 6,09561
1HpUi 442 6,23878
1daLP 440 5,35801
1dbro 526 6,13022
1deCx 526 5,96670
1df3C 526 6,72453
1dnhf 515 8,34164
1drNT 526 6,47546
1eFzn 485 8,57721
1fZmi 496 6,18985
1m7IY 498 6,43698
1mYJK 428 6,21463
1mhTs 430 5,75912
1mocS 338 6,60699
1n9Uj 427 6,41624
1ngI0 433 5,76162
1o7fh 426 6,67696
1obDo 406 5,95953
1rFhI 417 6,30631
29btV 434 8,64497
2Yqqz 414 5,50033
2cdD4 383 6,46091
2lWIG 437 5,96084
2od6V 495 7,03703
3B2IA 350 8,04548
3NV0y 485 6,38850
3PJMY 401 5,72712
3oXZy 443 8,80704
3oZja 451 6,34348
3qLAE 495 8,18499
3r5sc 427 6,41624
3rieT 254 6,97542
3sONN 495 7,03663
3tCM0 448 6,73811
3tJLD 426 6,67696
3uOGK 357 6,29443
3uQJX 495 7,06818
3v4F 392 6,18104
3vwXT 354 7,50635
3y5GK 320 6,51911
4UXKY 422 5,99358
4ZP7E 438 5,61520
4b9d 323 8,55923
4j8HK 435 5,60980
4jckm 434 8,65174
4tKsd 496 6,64848
4uNlG 437 8,92437
4ufXA 496 6,64996
4xD7i 318 8,72981
4xDRN 455 6,39401
4xoRD 442 5,97499
4xpBK 442 6,41834
4xqkF 507 8,72697
5Nx3Z 426 6,27761
5R6Et 433 5,89838
5R8Bx 426 7,57120
5Rp1m 432 6,42232
5Rx7C 433 5,89838
5SKw9 452 7,63231
5TO06 442 7,59559
5VJ3A 427 6,21474
5WDx6 433 5,89838
5Z1Ec 435 7,07994
5ZGEx 426 6,67696
61EwD 433 5,76151
61baL 426 6,41845
63b8X 405 6,03769
66IvN 426 6,41845
67vcR 426 6,21463
69zHK 427 6,41720
6Xqlr 492 5,46793
6Z920 306 6,29176
6ZkQW 306 6,29176
6aGXc 433 5,44497
6aTm3 426 6,67696
6as2G 426 6,21593
6coJu 433 5,89838
6fxv1 426 6,41845
6g5pZ 433 5,89838
6iRqp 452 9,19127
6mNk4 427 6,41686
6nBWT 375 6,40691
6nDcY 442 7,03959
6neIP 439 5,85057
6pGKz 426 6,67696
6pSJK 433 5,89838
6pvri 433 6,04576
6qJFe 426 7,57103
6r6hW 433 5,89838
6ru3H 433 5,64840
6tV9J 442 6,67934
6uD0o 433 6,04815
6v0tU 442 7,55421
6v7fx 433 5,89838
6vO6a 426 7,04442
79Ldj 496 6,44920
79PHE 413 5,40547
7BWvn 496 5,94743
7BbVK 438 6,77006
7M6ph 451 6,18956
7bjIB 451 6,18797
7mr5V 433 5,89838
7og7W 418 6,05849
7og9e 418 5,49192
7oqKf 416 8,60306
7ormO 437 5,95396
7rRQG 463 8,13773
7rTlJ 415 9,25987
7szTj 298 5,90912
7vEVa 268 9,42085
7vEXk 361 8,68662
7vEYI 389 8,55039
84HHI 452 8,13032
8fv1Z 486 7,56239
8mOyN 433 6,04576
8nvvf 430 5,33749
8ub8O 437 6,19280
9by2 440 6,61068
BKSi 434 5,90127
BNuR 433 5,89838
NO6L 433 6,22122
OuyD 507 8,52835
fZh7 433 6,58130
khUu 413 6,04491
n67g 445 5,92600
nbJ6 445 6,18320
nvBQ 441 7,10763
nz9Z 434 6,19280
oc2X 439 6,67082
olUu 439 6,06565
omNK 437 8,35441
ooEx 440 7,07136
ovDt 524 9,25710
oviY 438 8,94668
popB 451 6,18956
z1M9 412 6,14148
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_14180
2AXSB
 54 27,7% 403 5.851E-71
2 phalp2_39119
2teHi
 5 28,6% 398 3.366E-67
3 phalp2_29726
1lBT8
 1 28,5% 546 7.382E-66
4 phalp2_35035
7KLgC
 2 26,5% 464 7.759E-60
5 phalp2_17838
8LXT6
 17 24,1% 546 1.678E-58
6 phalp2_5670
4yIAx
 6 20,6% 479 2.006E-46
7 phalp2_29859
41oUZ
 2 19,7% 682 4.989E-46

Domains

Domains [InterPro]
Disordered region
Ami3
GLUCO
Unannotated
Representative sequence (used for alignment): netU (452 AA)
Member sequence: A0A2K9VH44 (458 AA)
1 452 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520, PF01832

Taxonomy

  Name Taxonomy ID Lineage
Phage Faecalibacterium phage FP_Mushu
[NCBI] 		2070185 Mushuvirus > Mushuvirus mushu
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MG711460 [NCBI]
CDS location
range 9870 -> 11246
strand +
CDS
ATGGCAAAATTGACACCAGACGCGACGAGGACGGAGCATGGCCTCGTCATCAACGAGAAGATCATCCCGTGGGGCGCGGTCTGGCCCAAGGACTCCGGCGCGTACAAGAAGGGCACACAGTACAAGGCCGACCGTCTGCTCTCGGGCGGCACGGGCAAGGTCAAGGGCGTGACCATCCACAACACTGCCGACCTGAAGAACGTGGAGGAGGACGCCGAGCAGTACACCCGTGCGACGTGGCCTAACGGCAACATGAACGACGCCCGCGTCCACTACTACGTCGACGACATCAACGCATGGCAGAACCTGAGAGAGGACGAGGTGGGCTGGCACGCGGGCGATGGCCGGAAGGCCACGGGCGGCAACGAGACGACGCTCTCCATCGAGATCATCATGGACGGCACGGGCAGCAAGGAAGACCGCAAGGCTGAGGAGAACGGCGTACTGCTGGCGGCGCTGCTGCTGAAGAAGCACGGCTTGAGCGTGAACGAGCTGTACACGCACAACCACTGGATGGGCCACCCGGACTCCATCGTGCAGGGCGCGAGGAAGAACTGCCCGCTGTACATCCTGCCTCATTGGGCGCAGTTCAAGCAGAAGGTCGCCGCGAAGCTCGCGGAGCTGAACGGCGGCGCGACCACCACGGAGACGGGCAAGACGGAGATCATGGGCAAGGCCAAGGCGAGCGCGCAGCAGATGGCGCTGTTCGCCCGATCCAAGAACGCGGAGCCGCAGCTCCCGGCGTGCTCGCTGGAGCAGCTGGCGCAGTTCTTCCTCGAAGAGGGCGAGGCCGAGGGCGTGCGCGGCGACGTCGCCTTTGCGCAGAGCCTCCACGAGACGGGCTTTTTCAAGTACGGCGGCATCGTGCTCCCCACGCAGAACAACTATGCGGGCATTGGGGCGCTGAACGGCAACGCCAAAGGACAGGCGGCGACCTTCCCCGACCCGCGCACGGGCGTCCGGGCGCAGATCCAGCACCTGAAGGCATACGCCTCCAAGGCGGCGCTCGTGAACGGGTGCGTCGACCCGCGCTTCTCCCTCGTGACACGGGGCTCGGCGCAGTATGTGGAATGGCTGGGCGCAAGCGACAACCCCAACGGCAAGGGCTGGGCCGTACCGGGCAAGGGCTACGGCAGCAAGATCGTCGCCCTGTTAGGTCAGATCATGGCGTTCGAGGTGCCGCAGCCGTCCACGCCGAGCGAGCCGGAGGAGCAGGAGCCGGAGTTCCCGGCGTATCAGTTAGAGGGGCTTGAAACGCTCGTGGAGGCTGACGTCATCAACTCCCCCGAGTTCTGGCGGCAGAAGTTCGGCGAGCAGGTGACGGTCGGCGAGTTGTTCGGCATCTTGGGGAAGCTCTTCACGAAAGCAAGCGAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0004040 amidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0001b2fc87_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (netU) rather than this protein.
PDB ID
netU
Method AlphaFoldv2
Resolution 90.33
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50