Protein

Protein accession
A0A0U4JE28 [UniProt]
Representative
8LXT6
Source
UniProt (cluster: phalp2_17838)
Protein name
Putative N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTKVIVLDAGHGRNTPGKRTPDGEREWTFNDKMLRAAQKHLTDNYQGFTIVRTDDPTGNTDVSLAARATRANNAKGDVFVSFHNNAFQGKWGSHGGTETYVPTPAANNPRSSALARSVHPKMQRAFGLRDRGIKAGNLYVIMNTKMPAILVEGAFMDSTTDIVKLRDDNVLRRAGEAIAEGVAEFLALSKKPQPVAPKPVAPTVRPTTNPEAAKVDVYRVRVDGTQVGAYGNPKNALDEVEKALKAGKKKVELERV
Physico‐chemical
properties
protein length:256 AA
molecular weight:27844,3 Da
isoelectric point:9,82
hydropathy:-0,55
Representative Protein Details
Accession
8LXT6
Protein name
8LXT6
Sequence length
556 AA
Molecular weight
60975,38030 Da
Isoelectric point
9,26174
Sequence
MYKIAICAGHGFNTPGKRTPDGEREWSFNNKLVVAFVNTMNQYENVTVKRYDDPTGKTDVPLITRTNGANAWGANIYISFHHNANTAKWGTWTGVETFYYPGSTTGLNLAKAIHPAVVQSYGLKDRGVKSGNLHIVRETKMPAILIEGGFMDSTIDIKKLRSDAVLKAAGENIAHAVAKYAGLKKKSSTSTPKPPTPTTPNAATYTVVKGDTLYGISQKFGISVDTIKSLNGLKTNTISIGQVLKVKKVEDVTADSFYRVFVNGKQVGAFSVKENAYEDGWKQYNSGKTDAYLVEPNGSKYTFNAHQSENPNKKQDETNSTKNPILGKSVVTSSQMVTLVKSKNPNAKNIEEIAEAFLKVGEKYGLRGDIAFCQSIIETGWFRFDGGTAVTPDQHNYCGLGVTSTGIKGAVFATIKDGVTAQIQHLYAYATKSALPAGETLLDPRFQYVTRGIAPNWEDLSNRWAMNANYGTQILNMYNQLLQVPASNTSPEKPNTPKPKEEKKDVLFTHSEINNKQYASVVIDGKGYIAVSDVADLLDLKVVFDKVTKEIIFIKK
Other Proteins in cluster: phalp2_17838
Total (incl. this protein): 17 Avg length: 410,2 Avg pI: 8,15
Protein ID Length (AA) pI
8LXT6 556 9,26174
1GGtO 483 7,15781
1LJpS 472 5,46901
36Dn5 377 7,60048
3NcEn 690 9,14150
4NP0T 690 6,43567
4lxDw 379 6,41146
4mtAe 379 7,59809
5V7hd 422 8,73329
7vAas 466 8,70950
Zkdg 382 9,09683
G3MB93 248 9,77703
A0A5Q2F3V6 267 7,02737
A0A6G6XUR0 274 9,44470
A0A6G9L8D2 268 7,02737
A0AA52HLC6 365 9,79302
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_35035
7KLgC
 2 28,0% 488 6.598E-76
2 phalp2_5670
4yIAx
 6 26,3% 538 1.812E-66
3 phalp2_14180
2AXSB
 54 24,5% 557 2.936E-60
4 phalp2_39119
2teHi
 5 25,5% 509 3.320E-59
5 phalp2_2869
netU
 143 25,8% 557 1.318E-51
6 phalp2_29726
1lBT8
 1 25,3% 497 1.517E-36

Domains

Domains [InterPro]
Ami3
LysM
Unannotated
GLUCO
Disordered region
Representative sequence (used for alignment): 8LXT6 (556 AA)
Member sequence: A0A0U4JE28 (256 AA)
1 556 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01476, PF01520, PF01832

Taxonomy

  Name Taxonomy ID Lineage
Phage Exiguobacterium phage vB_EauS-123
[NCBI] 		1775141 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KU160495 [NCBI]
CDS location
range 26667 -> 27437
strand +
CDS
ATGACAAAGGTAATCGTTTTAGACGCCGGACACGGACGTAACACGCCGGGCAAACGGACGCCGGACGGCGAACGCGAATGGACGTTCAATGACAAGATGTTGCGCGCTGCTCAAAAACATCTGACTGACAACTATCAAGGGTTTACCATCGTCCGAACGGACGACCCGACGGGCAACACCGACGTATCATTAGCAGCGCGCGCCACACGCGCGAACAACGCGAAAGGCGACGTCTTCGTTTCGTTTCATAACAACGCGTTCCAAGGCAAGTGGGGTTCCCACGGGGGAACGGAAACCTATGTACCGACACCGGCCGCGAATAATCCAAGATCGAGCGCGCTCGCCCGTTCGGTCCACCCGAAAATGCAACGCGCTTTTGGATTGCGCGACCGTGGTATCAAAGCCGGGAATTTATACGTGATCATGAATACAAAAATGCCTGCAATCTTGGTCGAGGGCGCGTTTATGGACTCGACGACCGATATCGTCAAGTTACGAGATGACAACGTACTAAGACGGGCAGGGGAAGCAATCGCGGAAGGTGTGGCCGAATTCCTCGCATTGAGCAAAAAACCGCAACCCGTCGCACCGAAACCGGTCGCGCCGACGGTCCGACCGACGACCAACCCGGAAGCTGCGAAAGTGGACGTTTACCGCGTCCGCGTGGACGGGACGCAAGTCGGGGCATACGGCAACCCGAAAAACGCGCTTGATGAAGTCGAGAAAGCGTTAAAGGCAGGTAAGAAAAAAGTAGAATTGGAGCGTGTGTAA

Gene Ontology

Description Category Evidence (source)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. N/A UniProt

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8LXT6) rather than this protein.
PDB ID
8LXT6
Method AlphaFoldv2
Resolution 82.00
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50