Protein

Protein accession
A0A0M4S688 [UniProt]
Representative
7sgWH
Source
UniProt (cluster: phalp2_12309)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNIVNMRGKLRSKGSYRDFGAASKTHIIVHHSLTKTGSAEAYARYHVDTLGWPGIGYHFVIEQDGTIKQNHSIGVMSYHVGNHNKYCVGICLTGDFRTQEPTTAQKNSLCQLVSYLQRNYPQLKTVKGHSDLRGYEWKECPCFDYAAVLREGNNNSNNKSKSQGVNIKVNTFNKVVTYEFGTALVPEMLGMMDALGYESRIISYGDKQGLVRFETAYRQGNELDRATAWLDARGLKYYYTKE
Physico‐chemical
properties
protein length:242 AA
molecular weight:27405,6 Da
isoelectric point:9,14
hydropathy:-0,57
Representative Protein Details
Accession
7sgWH
Protein name
7sgWH
Sequence length
389 AA
Molecular weight
43297,48150 Da
Isoelectric point
7,27047
Sequence
MAHFESLAEIKDIRGKTAKKGYYSRPPLGKKRSIAIHHSLTTSGSSAAFANYHVKTLGWPRVAYHFVIRKDGTIEWNHSLDIKSYHVGNSNEEAVGICLVGDFRTQHPTKAQLRSLKKLVTELKKDLPSYKVTKGHNEYPGYAWKACPEFDYKAALKAKVSKPSGTNNEPLLQAGDTFNAVKKLQQMLLATGEKLPKYGADGDFGTETEQAVKSFQRAQNITVDGVVGPETWEHLNKAISKDKDWKNKLAVVVADVMNVRHSPDMADDAIAGRVKKDEAFTIIDKVVASNGSTELYKLKSNLYISAHEDYTELKDVPEPEPRDELVIITHEGGLNVRDRLSFDDDAVVGQVQKGEAFTIADTHEVDGTPMFELKSGLFITAHEEYVKVR
Other Proteins in cluster: phalp2_12309
Total (incl. this protein): 3 Avg length: 333,7 Avg pI: 8,61
Protein ID Length (AA) pI
7sgWH 389 7,27047
A0A3G3BWP3 370 9,43090
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_8070
5Z13p
 106 26,7% 288 7.891E-12

Domains

Domains [InterPro]
Ami2
PG_1
Unannotated
Unannotated
Representative sequence (used for alignment): 7sgWH (389 AA)
Member sequence: A0A0M4S688 (242 AA)
1 389 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage phi4B1
[NCBI] 		1643324 No lineage information
Host Bacillus cereus
[NCBI] 		1396 Firmicutes > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group
Host Bacillus thuringiensis
[NCBI] 		1428 Firmicutes > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KT626446 [NCBI]
CDS location
range 20349 -> 21077
strand +
CDS
ATGAACATTGTAAATATGAGAGGGAAGTTACGTTCAAAAGGTTCGTATAGAGATTTTGGTGCGGCTAGCAAAACACATATCATTGTACACCATAGTTTAACGAAAACAGGGAGTGCAGAAGCTTATGCACGTTATCATGTTGATACATTAGGGTGGCCAGGCATTGGTTATCACTTTGTAATTGAACAAGATGGAACAATTAAGCAGAATCATAGTATTGGTGTTATGTCGTACCATGTGGGGAATCACAATAAGTATTGTGTAGGGATTTGTTTAACTGGTGATTTCCGTACACAAGAGCCGACAACAGCACAGAAAAACAGTCTATGTCAACTTGTTTCGTATTTACAACGTAATTACCCGCAATTAAAAACTGTGAAAGGGCATAGTGATCTAAGAGGTTATGAATGGAAAGAATGTCCTTGTTTTGATTATGCAGCTGTATTACGTGAAGGAAATAACAATTCAAATAATAAATCAAAATCTCAGGGAGTGAATATTAAAGTGAATACATTTAACAAAGTCGTTACGTATGAGTTTGGTACAGCATTAGTACCTGAAATGTTAGGAATGATGGACGCTCTTGGATATGAGTCCCGCATTATTTCTTATGGAGATAAGCAAGGTTTAGTTCGTTTCGAAACAGCATATCGCCAAGGAAATGAATTGGACAGAGCTACAGCATGGCTAGATGCAAGGGGACTTAAATATTATTACACAAAAGAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008270 zinc ion binding molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. N/A UniProt

Tertiary structure

PDB ID
upi000200f436_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7sgWH) rather than this protein.
PDB ID
7sgWH
Method AlphaFoldv2
Resolution 89.72
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50