Protein

Protein accession
A0A0K2FLH5 [UniProt]
Representative
7x2xX
Source
UniProt (cluster: phalp2_38227)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSFKMKYPIEKQYLTSGSKRRSGIKMPYVGFIVSHDTGNDGSTAKGNVGYYERSRNEMSASAHTFIDHCSIIECIPATTDKPEKAWHVLYNVTIDNQMFGGDANDIAIGVELCYSYKKGSINNKEAYKRYVWYHAYLCHKFNIDPRKKIVGHNELDPTRKTDPFKNALKIMGINKAQFIQDVVDELADCLATESLGVNITKESEDDAPMKLEQWQKDVIIEGVKKWSKVNDSDGSPIINSPDYWIKKVNDGTLTASELSTLTFTVVSRNQK
Physico‐chemical
properties
protein length:271 AA
molecular weight:30654,4 Da
isoelectric point:7,62
hydropathy:-0,56
Representative Protein Details
Accession
7x2xX
Protein name
7x2xX
Sequence length
339 AA
Molecular weight
37326,88160 Da
Isoelectric point
5,75219
Sequence
MAFKMNYTIEQKLLPYKSKRRSGAAMARVGFIVAHDTGNDGSTAAGNVNYYTSSADDISASAHTFIDHKQILECIPATIGSPEKAWHVLYDITLDNQIYGDDANDTAIGVELCYSYKKGSINNEEAYKRYVWYLAYLCYKFTLNPLKDIIGHNTLDPKRKTDPINALQHLGKSFNNLILDVAAQYKECSGKTLEYSPNPPSGTILCKGSKGEAVRKLQLKLNTIGFLLDADGDFGSGTEEAVRSFQQMAGLAIDGQAGNITMATIDGWIKEAADKMAEGENEKLNLLQWQVDMLAEALSKLQAAGMFESKDWITKTKNNTLTTSELSFLNTIILSKGVN
Other Proteins in cluster: phalp2_38227
Total (incl. this protein): 21 Avg length: 320,0 Avg pI: 7,67
Protein ID Length (AA) pI
7x2xX 339 5,75219
1mrSI 353 6,13284
2G93Z 313 9,80585
3Ndb3 341 9,43097
4C6qZ 336 9,13357
4lHMh 350 9,34580
72bKn 343 7,05164
72ted 303 5,34857
7crSP 258 8,54053
7gBRr 358 4,99998
7iHK2 336 8,99961
7rXYb 351 9,41350
7srfY 275 8,04619
7wNox 346 9,62824
7xkti 243 6,14364
7y7S9 352 6,18797
7y7Tw 326 9,28224
7yZN8 304 5,28980
81dMH 361 8,62208
A0A0K2CPP8 260 6,23481
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_8205
7kqpf
 6 26,8% 261 3.069E-29
2 phalp2_36672
45lpM
 229 26,9% 256 5.626E-29
3 phalp2_17898
EGF4
 130 23,2% 323 1.570E-27
4 phalp2_33823
1lgPb
 23 25,1% 266 4.814E-25
5 phalp2_7264
3WBGP
 11 28,7% 278 1.427E-22
6 phalp2_22201
6VaYI
 26 25,1% 282 2.550E-18

Domains

Domains [InterPro]
Ami2
PG_1
Unannotated
Representative sequence (used for alignment): 7x2xX (339 AA)
Member sequence: A0A0K2FLH5 (271 AA)
1 339 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Brevibacillus phage Sundance
[NCBI] 		1691958 No lineage information
Host Brevibacillus laterosporus
[NCBI] 		1465 Firmicutes > Bacilli > Bacillales > Paenibacillaceae > Brevibacillus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KT151959 [NCBI]
CDS location
range 63430 -> 64245
strand +
CDS
ATGTCTTTTAAAATGAAATATCCCATTGAGAAACAATATCTCACTTCTGGAAGCAAAAGACGCTCTGGGATTAAAATGCCTTATGTTGGTTTTATTGTATCTCATGATACAGGTAATGATGGGAGTACCGCAAAAGGGAACGTTGGCTACTATGAGCGTTCTCGAAATGAAATGAGTGCATCTGCACACACATTTATTGACCATTGCTCTATCATTGAATGTATACCTGCCACAACAGACAAACCAGAGAAAGCGTGGCATGTGCTTTATAACGTTACCATTGACAATCAGATGTTTGGCGGAGACGCCAACGATATTGCCATTGGGGTGGAACTTTGCTACAGCTACAAGAAGGGTTCTATTAACAATAAGGAAGCGTATAAGCGTTATGTATGGTATCATGCATATTTGTGTCACAAGTTCAATATCGACCCTCGTAAGAAGATTGTAGGTCACAATGAACTTGACCCTACTCGTAAGACAGACCCATTCAAGAATGCATTAAAGATTATGGGTATTAATAAAGCACAGTTCATTCAGGATGTCGTTGATGAACTGGCTGATTGTTTGGCTACTGAGTCACTTGGAGTAAATATTACAAAAGAAAGTGAGGACGATGCACCTATGAAATTAGAACAATGGCAAAAAGATGTGATTATTGAGGGAGTAAAGAAATGGAGCAAGGTGAATGACAGTGATGGAAGCCCAATTATCAATAGTCCTGACTATTGGATTAAGAAAGTTAATGATGGAACGCTTACTGCTAGCGAATTATCTACTCTGACTTTTACAGTTGTTAGCAGAAATCAGAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0006bc2f8a_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7x2xX) rather than this protein.
PDB ID
7x2xX
Method AlphaFoldv2
Resolution 85.86
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50