Protein

Protein accession
A0A6M3SV45 [UniProt]
Representative
7Qd9Y
Source
UniProt (cluster: phalp2_12024)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNRNPGHRGDPLFLVEVLRAFGVDTREYSGWRNRGHGDFNIIWGIVIHHTGGNNASAASIANGSPGLAGPVSQIHLDRNAVATVVAAGIAWHAGVGSWPGIQTNNANAVTIGIEANSDGVTPWPPKMLDAYYRICAAICWYLGLPATRVIGHKDWAKVQGKWDPGLINVKDFQTQVQRYIDNPPFNQAPQTPAEPVTEAGEPMQFWDTKIASLVTPGKSFARKDFIQLIDYHATLADQQSKASVEASKQVLDEMRLLREDIAALARAIAGKEAGK
Physico‐chemical
properties
protein length:275 AA
molecular weight:29851,4 Da
isoelectric point:7,10
hydropathy:-0,25
Representative Protein Details
Accession
7Qd9Y
Protein name
7Qd9Y
Sequence length
281 AA
Molecular weight
31072,86700 Da
Isoelectric point
5,91583
Sequence
MAFQPNPGHRGDPLFLVDVLRAFGVKVKEFDAWRDRGQGDFFTIWGVIAHHTGSNNASAASIAYGHEGLKGLLSQIHLDRNGVATITGAGIAWHAGVGSWPGIQTNNANAVTIGVEANSDGVSPWPPEMLDAYHRICAAICWFLGHSSLRTIGHKEWAKVQGKWDPGGIDMAQFRAKVQYYIDHPPFMPPPQPVTEAGEPMAFWDDQVPTILSPEKSFPRHEYLQLIDMHAFLANTQSKQVLDQMKLVRDDNQRILNEMQLMRDDLSELATAVLSGVKKDG
Other Proteins in cluster: phalp2_12024
Total (incl. this protein): 81 Avg length: 339,0 Avg pI: 7,36
Protein ID Length (AA) pI
7Qd9Y 281 5,91583
1c3hj 278 8,92798
1dOUK 266 9,55887
1j14b 279 9,69264
1j1d3 289 9,51523
2aM0G 263 6,74852
4VdLQ 271 7,86374
5tkT 256 8,93063
6YX41 263 7,14332
6kp0P 259 6,23316
75QP9 263 7,14241
76Oju 314 9,31589
7BkpA 302 9,69065
7Qdd8 281 6,03877
7RbJQ 255 6,54384
7cxQm 259 8,38813
7es7n 296 6,16131
7nJyc 261 7,92073
7op4p 286 9,30003
7qV41 288 9,73223
7uWPG 256 6,44045
7ubo9 259 6,93574
7ui1m 302 9,43400
7uuKM 257 8,60642
7zBmK 256 8,38097
7zBuu 255 6,35195
8MOKF 275 7,09796
8bq98 259 6,05150
8pHyG 271 9,44560
BPte 259 6,23316
MZdF 259 6,23498
NfcR 239 6,03144
z4z2 259 6,05400
A0A649VB80 427 7,76622
A0A5J6D9U1 428 7,77561
A0A2U8UKP6 384 5,91878
A0A649V6I9 427 7,26694
A0A2P1JY73 427 7,26973
A0A410TBB4 428 8,27195
A0A5J6TQU5 368 7,26928
A0A514CXM0 428 7,33964
A0A515MH83 355 5,78589
A0A5P8D791 428 7,28121
A0A649VDE0 429 7,81694
A0A4Y6EFS0 384 6,05269
A0A5J6TC71 368 7,26928
A0A5J6TJN1 368 7,26928
A0A5J6TS12 368 7,27666
A0A5J6TXU3 368 7,26928
A0A649V9M9 428 7,80920
A0A649VG24 368 7,26854
A0A6G6XLI9 384 6,14375
A0A7D5FR85 427 7,76622
A0A7D5JSR0 392 6,43192
A0A7T0M233 275 8,83206
D4P7C1 265 5,84637
D4P7N0 265 5,97710
A0A9E7ITX9 384 6,20531
A0A9E8M6M0 384 5,87496
A0AA96KPW9 291 5,81459
A0A8F3E6J9 428 7,26797
A0A9E7MDB5 428 7,79779
A0A9E7QAU5 429 8,53041
A0A9E7QJI7 428 7,79779
A0A9E7QPE2 368 6,95621
A0A9E7QQJ7 369 6,76420
A0A9E8S3B7 428 7,80475
A0AA49BNH6 368 7,27587
A0AA96GZL3 368 7,27666
A0AA96H231 427 6,95086
A0AA96H878 428 7,01816
A0AA96HH75 369 6,76420
A0AA96HV58 368 6,95621
A0AA96JZS3 368 6,95621
A0AA96KMF8 368 6,95621
A0AA96SFF7 428 7,26797
A0AAE7SMF9 428 7,26797
A0AAE7SQW0 428 7,79779
A0AAE7VGS9 366 7,25495
A0AAU8GTB4 427 7,76514
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_36860
7gDKs
 246 46,4% 183 1.046E-54
2 phalp2_30257
4gSoB
 83 38,9% 203 3.087E-35
3 phalp2_32562
1EaZA
 16 37,6% 194 1.450E-34
4 phalp2_22551
1qQEX
 38 29,3% 259 1.494E-32
5 phalp2_30398
4MRRT
 4 31,3% 220 6.047E-31
6 phalp2_37674
4urEs
 29 35,0% 214 1.524E-30
7 phalp2_30116
3dGzs
 45 34,2% 225 2.073E-30
8 phalp2_39896
1eFLE
 1 34,3% 189 1.318E-27
9 phalp2_7405
4FizF
 295 34,1% 208 6.109E-27
10 phalp2_33273
5GVax
 183 32,0% 209 5.212E-26

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Representative sequence (used for alignment): 7Qd9Y (281 AA)
Member sequence: A0A6M3SV45 (275 AA)
1 281 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Gordonia phage Secretariat
[NCBI] 		2725616 Secretariatvirus > Secretariatvirus secretariat
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MT310850 [NCBI]
CDS location
range 4290 -> 5117
strand +
CDS
GTGAATCGAAATCCTGGACACAGAGGCGATCCTCTGTTTCTAGTAGAAGTGCTTCGCGCTTTTGGCGTGGATACCAGAGAATACAGTGGCTGGCGAAACCGAGGACATGGTGATTTCAACATCATCTGGGGCATTGTCATTCATCACACTGGTGGAAACAATGCGTCTGCCGCATCGATTGCCAACGGCAGTCCTGGTCTAGCCGGCCCTGTGTCTCAGATCCACTTGGATCGAAATGCTGTGGCAACAGTTGTCGCTGCTGGAATCGCTTGGCACGCTGGTGTCGGTTCTTGGCCTGGGATCCAGACGAACAATGCCAATGCAGTCACGATCGGTATCGAAGCAAACTCGGATGGCGTCACTCCGTGGCCGCCGAAGATGCTTGATGCCTACTACCGTATCTGCGCGGCCATCTGCTGGTACCTCGGCCTTCCTGCGACTCGAGTGATCGGTCACAAGGACTGGGCCAAGGTTCAAGGCAAGTGGGATCCGGGTCTGATCAATGTTAAGGACTTCCAAACACAAGTCCAACGTTACATCGACAACCCGCCCTTCAACCAAGCGCCGCAGACACCGGCCGAACCAGTCACAGAAGCAGGAGAACCAATGCAGTTTTGGGATACAAAGATCGCGAGTCTCGTTACTCCGGGCAAGTCGTTCGCTCGCAAGGACTTCATTCAGCTGATCGACTACCACGCCACTTTGGCGGACCAGCAGTCGAAGGCAAGCGTGGAGGCCAGCAAGCAGGTGCTCGACGAGATGCGTCTCCTTCGGGAGGACATCGCCGCCCTAGCCCGAGCGATCGCCGGCAAGGAAGCGGGTAAGTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7Qd9Y) rather than this protein.
PDB ID
7Qd9Y
Method AlphaFoldv2
Resolution 93.67
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50