Protein

Protein accession
F8HGP2 [UniProt]
Representative
Omza
Source
UniProt (cluster: phalp2_568)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MATDNDIIQFAENLADAGVGTDADGAWGTQCVDLPNSISINFFGRALWGNAIDLLNSAAEAGYEVEYNQVGNLDSRPRRGAVFVMDTTYIAGHPYGHTGLVIEDSDGYTMRTIEQNIDGNADSLYVGGPARYNTRNFDGIVGWFYFPTDNQSQAPAPTLTPFDGIITINEETGTFTVEVSALNVRAGAGLGAEIVAVYGAGETINYDGWCDVDGYIWISYISWSGNRRYVAVGQSENGRRVTSFGSFA
Physico‐chemical
properties
protein length:248 AA
molecular weight:26737,9 Da
isoelectric point:4,13
hydropathy:-0,17
Representative Protein Details
Accession
Omza
Protein name
Omza
Sequence length
146 AA
Molecular weight
16002,64590 Da
Isoelectric point
4,56368
Sequence
MVRTIDLVNEAKRIANLGIGVDQDGAYGTQCVDLPNYLSSYFFGKTLWGNAIDLLNSAAALGYKVEYNVVGDLNSRPKAGAVFVMDTTYTAGHSYGHTGLVIEDSDGYSMKTIEQNVDGNWDSLYVGGPARYVSRDFEGIVGWFYF
Other Proteins in cluster: phalp2_568
Total (incl. this protein): 65 Avg length: 161,5 Avg pI: 6,13
Protein ID Length (AA) pI
Omza 146 4,56368
1lGDD 126 4,59494
5OneZ 137 5,09501
5PzQJ 159 4,36770
5ZKT3 149 4,70851
6YEHs 145 4,24595
6YQ2s 218 4,46228
6YVVH 150 4,44972
6Z0SV 218 7,78045
6ZBS2 103 4,67787
6ZpdC 123 4,37191
6acA2 149 4,09396
6eBap 137 5,42468
6sqe3 209 4,73244
70tIQ 110 4,55476
718Pq 142 6,99315
718u2 142 6,99071
71h3F 164 5,27872
75Y12 171 5,87524
75Y17 171 5,74463
75Y2E 171 5,65255
7MWlv 218 7,62986
7MzIO 145 4,24595
7P4Qq 152 6,24907
7P5mK 129 7,69892
7P9Tm 178 4,75705
7Ql1X 171 5,90639
7RlqS 186 10,33842
7RnS4 121 9,65815
7RpBC 115 9,38004
7gkp6 115 9,47745
7gs5F 94 9,37230
7gtKq 122 9,80443
7j1NW 137 6,24981
7qThE 121 9,80579
7qsXk 115 9,06762
7qtQA 115 9,30016
7qtup 115 9,30016
7qtyF 115 9,30016
7yXmA 117 9,75737
7zGgi 132 9,55107
8JuTO 171 5,65255
8MgDt 171 6,04945
CETw 102 5,99455
D5rG 151 4,39237
EyVn 157 4,47427
HNnU 141 4,11386
HtwU 136 4,42261
N4m1 160 7,85175
NcAU 154 4,22390
NcFc 156 4,19520
NfM8 163 4,05003
Oq8d 161 7,84646
p65e 170 6,98451
wUHF 188 4,80650
wko7 121 9,79373
A0A4D5ZZ23 248 4,29938
A0A4D6B540 250 4,18462
A0A4D6AY01 250 4,19241
A0A4D6ARP3 246 4,29313
A0A8S5QNY4 250 4,17121
A0A8S5TPK4 250 4,28170
A0A8S5TV67 250 4,17121
A0A8S5TV78 248 4,11272
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_25333
7ocj2
 56 52,4% 143 8.798E-52
2 phalp2_14804
73AW6
 35 30,2% 139 2.026E-33
3 phalp2_14686
6m7Es
 60 32,5% 163 2.524E-32
4 phalp2_32089
64JDG
 3 44,8% 98 1.557E-27
5 phalp2_27772
7kL3N
 52 27,9% 136 2.723E-17
6 phalp2_21041
fs6P
 6 29,9% 127 6.111E-13
7 phalp2_35756
4f4tw
 5 26,8% 134 8.979E-11
8 phalp2_13870
7vCZb
 24 33,0% 103 8.979E-11
9 phalp2_20203
84gv6
 6 28,1% 142 8.979E-11
10 phalp2_5557
3TDb1
 10 25,0% 128 6.959E-09

Domains

Domains [InterPro]
CHAP
Representative sequence (used for alignment): Omza (146 AA)
Member sequence: F8HGP2 (248 AA)
1 146 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Streptococcus phage YMC-2011
[NCBI] 		1051631 Aliceevansviridae >
Host Streptococcus salivarius 57.I
[NCBI] 		1046629 Firmicutes > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
CP002889 [NCBI]
CDS location
range 5855 -> 6601
strand +
CDS
ATGGCAACAGATAATGACATCATCCAATTTGCGGAAAACCTAGCCGACGCTGGAGTCGGTACCGATGCAGACGGAGCGTGGGGAACGCAATGCGTTGACCTGCCTAACTCTATCTCAATTAACTTCTTTGGCCGCGCTCTTTGGGGCAACGCTATTGACTTGCTCAACTCAGCGGCAGAAGCAGGCTATGAAGTCGAGTATAACCAAGTGGGCAACCTTGACAGTCGTCCACGTCGTGGGGCTGTATTCGTCATGGATACTACTTACATCGCAGGGCACCCATACGGGCACACTGGTCTGGTTATCGAAGATTCGGACGGCTATACCATGCGAACTATCGAGCAGAATATTGACGGTAACGCTGATAGCTTGTATGTTGGTGGCCCCGCTCGTTACAATACACGCAATTTTGACGGCATTGTAGGTTGGTTCTATTTCCCAACAGATAACCAATCCCAAGCCCCTGCACCAACTCTGACCCCGTTTGATGGTATAATTACTATTAACGAGGAAACCGGGACATTCACGGTTGAAGTCTCAGCTCTTAATGTTCGAGCTGGTGCTGGTCTAGGTGCTGAAATTGTGGCAGTCTATGGAGCCGGTGAAACTATCAACTATGATGGTTGGTGTGACGTTGACGGCTATATCTGGATTAGCTACATTAGCTGGTCTGGTAATCGTCGCTATGTTGCAGTCGGTCAATCAGAGAATGGTCGACGTGTCACGTCATTCGGTTCATTCGCTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0016787 hydrolase activity molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000217a919_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (Omza) rather than this protein.
PDB ID
Omza
Method AlphaFoldv2
Resolution 94.81
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50