Protein

Protein accession
R9TQ04 [UniProt]
Representative
4lGtr
Source
UniProt (cluster: phalp2_1931)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGRGVTRMSYKIVQKGNKHTNSSSRDGHVPYVIVNHISAGSMGSMDNWFTSSGNKVSSAHFGVSKKGEIHQYVPIERMAWANGLSKWEQEKADAAVVRNNPSLNPNKYTVSIEHEGTDGQLTDAQFAASVWLHKYIQSEIKRLYGRDMKLDEYHVIGHFQVDPKRKPNCPGPKFPWDQLYKELKKVGAAKVEQQKVKVLVNGKKVADGYLDGGTTYTPSRAVAEALGASVRWDKENLTVLITKEEK
Physico‐chemical
properties
protein length:246 AA
molecular weight:27501,9 Da
isoelectric point:9,47
hydropathy:-0,66
Representative Protein Details
Accession
4lGtr
Protein name
4lGtr
Sequence length
277 AA
Molecular weight
31592,18540 Da
Isoelectric point
6,76045
Sequence
MTFQIVWRGNEHTNSSSRDGHVPIGICNHISAGSMSSMRSWFTSPNNTVSSAHYGISKTGEIDQYVDLRRMAWTQGITADQLQYVTAQIVKDYCSRAGRFVNPNKFLVGIEHEGTDGRLTDAQLEASIWLHVHIRDEITRIWGEKYRFPLNSYTVVGHFQIDPKRKPLCPGPYFPWEQLYTALWEADNMTKDNEQDKRIAALEGRVAGLEKSLQQLAAQRDMEAPAWAKPALDYYGQFIDTKTGSLDFWRTLTIMHRREVGRIVAPETKPDDRKGGD
Other Proteins in cluster: phalp2_1931
Total (incl. this protein): 11 Avg length: 250,5 Avg pI: 8,42
Protein ID Length (AA) pI
4lGtr 277 6,76045
2AXoQ 239 9,28604
7925L 239 7,13394
7cQnY 239 8,82361
7gdtR 320 6,31739
7nTJ8 235 9,00954
7yQb9 238 8,77384
7z04Q 238 8,82922
7zJT3 238 8,72691
8LTPP 246 9,47178
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_24509
4NPVU
 68 45,0% 202 4.154E-65
2 phalp2_28639
8jnfC
 92 32,0% 240 1.180E-43
3 phalp2_28952
5IRWG
 1 31,6% 183 7.549E-38
4 phalp2_30952
173qx
 118 37,8% 169 2.078E-33
5 phalp2_28537
40K7Z
 71 33,1% 187 1.874E-26
6 phalp2_27234
3Qd6L
 2 29,2% 202 3.909E-23
7 phalp2_12587
1lnEh
 1 33,9% 168 9.752E-23
8 phalp2_9423
jh6D
 5 32,0% 178 3.747E-21
9 phalp2_16178
4A1r9
 27 28,9% 252 3.517E-19
10 phalp2_3532
4weez
 8 26,7% 172 1.308E-17

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Representative sequence (used for alignment): 4lGtr (277 AA)
Member sequence: R9TQ04 (246 AA)
1 277 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Paenibacillus phage PG1
[NCBI] 		754053 No lineage information
Host Paenibacillus glucanolyticus
[NCBI] 		59843 Firmicutes > Bacilli > Bacillales > Paenibacillaceae > Paenibacillus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
HQ332138 [NCBI]
CDS location
range 13882 -> 14622
strand +
CDS
GTGGGGAGGGGAGTGACAAGGATGTCGTACAAGATAGTACAGAAGGGGAACAAACACACAAACAGCAGCAGTAGAGATGGACATGTGCCTTATGTGATCGTGAACCACATTTCAGCCGGTTCTATGGGAAGCATGGATAATTGGTTCACTTCTTCAGGTAACAAGGTAAGTAGCGCACACTTCGGCGTTTCTAAGAAAGGTGAGATCCATCAATACGTTCCGATTGAGCGGATGGCATGGGCCAACGGTCTGAGTAAGTGGGAACAGGAAAAAGCTGATGCCGCTGTTGTGCGAAACAATCCTAGCCTTAACCCGAATAAATACACTGTTAGTATCGAGCATGAAGGCACTGACGGGCAACTGACGGACGCACAGTTCGCCGCATCGGTTTGGTTGCACAAATACATCCAGTCCGAAATAAAACGGCTGTATGGGCGCGATATGAAGCTTGACGAGTATCATGTGATTGGACATTTCCAAGTGGATCCTAAACGAAAGCCGAATTGCCCTGGTCCTAAGTTCCCATGGGATCAGTTATACAAAGAATTAAAGAAAGTTGGTGCTGCTAAAGTGGAGCAACAGAAAGTGAAGGTCCTTGTAAACGGCAAGAAGGTTGCAGATGGATACTTAGACGGTGGAACAACGTACACGCCTTCTAGGGCGGTTGCAGAAGCTCTAGGAGCGTCCGTTAGATGGGATAAAGAGAACTTAACAGTGCTTATCACAAAGGAGGAAAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi00034282bf_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (4lGtr) rather than this protein.
PDB ID
4lGtr
Method AlphaFoldv2
Resolution 92.79
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50