Protein

Protein accession
O48471 [UniProt]
Representative
2AXSB
Source
UniProt (cluster: phalp2_14180)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSKLVWLDAGHGGKDSGAAANGIKEKDIVLKIVKKVKSILTSRYEVAVKLTRDSDVFYELIDRARKANAAKADLFVSVHINATPGGKGFETYRYVKTSASSSTGQQQKVLHDAIYKRIKKYGIKDRGEKAADLSVLRNTSMPAVLTENLFIDNKDEAALLKKDSFLNDVAEGHAEGIAEILNLKKKSGGSAPKKEDKPSSGKTKMVIVKDNPDGFLWVYNKADWNARYKKVKPDEAFTIDKTVTVNGSKMYKLKSGLYITAASKYVTVKEK
Physico‐chemical
properties
protein length:271 AA
molecular weight:29882,0 Da
isoelectric point:9,67
hydropathy:-0,49
Representative Protein Details
Accession
2AXSB
Protein name
2AXSB
Sequence length
465 AA
Molecular weight
49765,67200 Da
Isoelectric point
9,11056
Sequence
MKKVWIDAGHGGKDPGATGNGVTEKNVALRVAVEIGEQLERNYDGVQCLLSRSTDVYLTPKERTDKANAAGADVLISIHCNAGGGAGGFESYTYSGTKDAATTEFKNTIHTEVMGRLKQFGVNDRGQKKKDLHMCRESRMPAVLTENLFVDVTADAARLKRPEVIEAIVSGHVSGVAKFLKLQPKKAQQQPTGRGTNILDAASATVEQAKAWARSNKAPTEFVALADLYWEMAPARSGIDPAIAYVQFGHETGFLYRDGKSNAGIDASYCNPCGLKITQGGGDTQASAHKRFANWREGITAHLDHLALYAGAAGYPKKDTPDPRHFGYLYGTAKTLEQLGEKWAPSSSYGTNLVGKYAKLRGTAAVDKPTNEIKGDSATVELDGKKIAEGTFLNGLVTIPVRQLAIALGATVGYDRKKATVNGKVIVGSQMVGDTAYAPVREVVEAAGYRVTGWDGLNRKVSVSK
Other Proteins in cluster: phalp2_14180
Total (incl. this protein): 54 Avg length: 408,8 Avg pI: 7,85
Protein ID Length (AA) pI
2AXSB 465 9,11056
10oGR 319 8,49972
10qre 378 8,63852
13fjp 423 5,67625
1crdt 402 7,67175
1knph 402 8,26145
2CkXb 438 9,50427
2JrGL 524 9,20513
2Pa98 384 5,63436
38xM9 412 5,13025
3bTHN 423 5,98443
49zx 369 9,25058
4Tljo 422 6,37946
4jCI8 395 8,43931
4tIUh 481 5,81738
4ucVg 485 6,87476
4ukIV 480 5,82130
5NVA8 410 6,76489
5WmgE 469 6,75750
6YDH1 335 8,44022
702N9 410 8,25758
70te0 469 6,91375
73fwY 409 8,55059
7KBwS 465 9,09599
7NiQU 469 6,70265
7OdXC 410 8,81826
7aLQG 469 8,32101
7d2h8 419 5,82125
7e46i 418 8,84166
7or93 410 7,09370
7rHNd 469 9,16291
7rHTH 467 8,80034
7scey 475 8,61667
7tBTv 421 6,73306
Bv03 391 7,67778
DClo 469 6,70322
KQra 469 6,91392
MVur 410 8,44441
OLY6 410 8,43596
Ohpq 410 8,96080
aZi0 412 6,60636
gUan 465 8,87725
lDDv 423 6,56436
yZRg 414 7,61270
z1DK 471 6,37099
zgHS 404 8,66785
A0A5H2QFX3 238 9,63553
A0A5P8PHQ8 270 9,59517
A0A5P8PI51 271 9,68484
A0A5P8PJT4 271 9,68484
J9PV86 237 6,21906
Q5YA51 355 9,73842
A0AAE7RDU3 420 8,17274
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_5670
4yIAx
 6 33,4% 479 5.589E-107
2 phalp2_17838
8LXT6
 17 24,2% 561 7.402E-72
3 phalp2_2869
netU
 143 25,5% 411 1.258E-66
4 phalp2_39119
2teHi
 5 27,3% 435 1.258E-66
5 phalp2_35035
7KLgC
 2 24,5% 436 2.805E-61
6 phalp2_2700
7bONi
 21 21,4% 467 1.876E-30

Domains

Domains [InterPro]
Ami3
Unannotated
Unannotated
Representative sequence (used for alignment): 2AXSB (465 AA)
Member sequence: O48471 (271 AA)
1 465 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage SPP1 (Bacteriophage SPP1)
[NCBI] 		10724 No lineage information
Host Bacillus subtilis
[NCBI] 		1423 Firmicutes > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus subtilis group

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
X97918 [NCBI]
CDS location
range 20842 -> 21657
strand +
CDS
ATGAGTAAATTAGTTTGGTTGGATGCAGGTCATGGCGGAAAAGATTCTGGAGCGGCGGCGAACGGTATCAAAGAAAAGGATATCGTCCTTAAAATCGTCAAGAAAGTAAAGTCAATTCTCACGTCCCGTTATGAAGTGGCTGTTAAACTCACAAGGGATTCAGATGTATTTTACGAGTTGATTGACCGGGCTAGAAAAGCGAATGCGGCAAAGGCAGACTTGTTCGTTTCTGTTCATATCAATGCCACGCCGGGCGGAAAAGGATTCGAAACATACCGCTATGTGAAAACGAGTGCTTCAAGCTCAACGGGACAACAACAAAAAGTTTTACATGACGCCATTTATAAGCGCATCAAAAAGTACGGCATCAAAGACCGAGGCGAAAAAGCGGCTGACCTGTCAGTTCTTCGCAATACGAGTATGCCAGCGGTTCTGACTGAGAACTTGTTTATTGACAACAAAGATGAAGCGGCGTTGCTCAAAAAAGACTCTTTCCTTAATGATGTGGCTGAGGGTCATGCGGAAGGTATCGCGGAAATCCTTAATCTGAAAAAGAAAAGCGGCGGCTCTGCACCTAAGAAAGAGGACAAGCCATCTAGTGGGAAAACAAAGATGGTTATTGTAAAAGACAATCCAGACGGGTTCTTATGGGTATACAATAAAGCGGATTGGAACGCCCGATATAAAAAAGTTAAACCGGACGAAGCTTTCACCATTGATAAAACTGTCACCGTGAACGGTTCGAAAATGTACAAGCTTAAATCCGGCCTGTACATCACGGCGGCAAGTAAATATGTAACGGTAAAGGAGAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0031640 killing of cells of another organism biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0046729 viral procapsid cellular component None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.- None None N/A UniProt

Tertiary structure

PDB ID
O48471
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi000009b6c9_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (2AXSB) rather than this protein.
PDB ID
2AXSB
Method AlphaFoldv2
Resolution 89.99
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50